Use of Atg5 transient silent vector in relieving organelle localization protein degradation

Abstract

The invention relates to the field of plant genetic engineering, in particular to use of an Atg5 transient silent vector. According to the use of the Atg5 transient silent vector, the Atg5 transient silent vector is infiltrated with endoplasmic reticulum, mitochondria, peroxisome or a plastid GFP expression vector, and use of the Atg5 transient silent vector in relieving protein degradation of different organelle localization is revealed. The Atg5 transient silent vector can act on the organelle region and promote accumulation of an organelle foreign protein. It is shown that an siatg5 silentautophagy pathway involves endoplasmic reticulum, plasmid, mitochondria, peroxidosome and other most cell sites, and an siatg5 silent vector can regulate the autophagy pathway of various sites and organelles in plant cells. According to the use of the Atg5 transient silent vector, great significance for elucidating a molecular mechanism of the Atg5 gene regulated autophagy pathway in influencing foreign protein expression is achieved, and guiding significance for improving expression of plants as foreign proteins of a bioreactor is achieved.

Description

technical field

[0001] The invention relates to the field of plant genetic engineering, in particular to a transient silencing vector of Atg5, a key gene of autophagy pathway. The silencing vector can lead to Atg5 gene silencing, inhibit the autophagy pathway, and relieve the degradation of proteins located in different organelles, thereby promoting the accumulation of foreign proteins. Background technique

[0002] Using gene recombination technology to express and produce foreign proteins by using organisms as bioreactors is a hot issue in current biotechnology research. Commonly used exogenous protein expression systems mainly include prokaryotic expression system, yeast expression system, insect expression system, mammalian expression system and plant expression system, etc. Different expression systems have different expression characteristics. Compared with other expression systems, the plant expression system has the following advantages: (1) As a eukaryote, plants h...

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