Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Primer composition and kit for detecting hypertension medicine related genes

A primer composition, primer set technology, applied in recombinant DNA technology, microbial assay/inspection, biochemical equipment and methods, etc., can solve problems such as limitation and high price

Inactive Publication Date: 2018-09-14
宁波美丽人生医学检验所有限公司
View PDF5 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, similar kits currently on the market are expensive and have limited applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Primer composition and kit for detecting hypertension medicine related genes
  • Primer composition and kit for detecting hypertension medicine related genes
  • Primer composition and kit for detecting hypertension medicine related genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0097] 1. Sample processing and nucleic acid extraction:

[0098] Use a commercial DNA extraction kit to process the sample. For specific operations, refer to the kit instruction manual. The concentration and purity of the extracted DNA need to be measured with a UV spectrophotometer. The DNA OD 260 / OD 280 The value of DNA should be between 1.8 and 2.0, and the concentration should be between 5 and 50 ng / μL. If the DNA quality of the sample is unqualified, it should not be used for detection. If it is lower than 5 ng / μL, the nucleic acid should be extracted again, and if it is higher than 50 ng / μL, it should be properly diluted. To the specified concentration range, the extracted DNA should be detected immediately, otherwise it should be stored below -20°C, and the storage time should not exceed 6 months. The quality control product of the kit was thawed at room temperature before use, vortexed for 10 seconds, and centrifuged at 2000rpm for 15 seconds before use.

[0099] 2...

Embodiment 2

[0117] A primer composition for detecting hypertension drug-related genes includes NPPA primer set, ADRB1 primer set, CYP2D6 primer set, AGTR1 primer set, CYP2C9 primer set, CYP3A5 primer set and GNB3 primer set;

[0118] The NPPA primer set includes the following primers:

[0119] NPPA-FW: 5'-CTGTGTTCCTCTTTGCAGTAGT-3';

[0120] NPPA-FS1: 5'-TGTGTTCCTCTTTGCAGTATC-3';

[0121] NPPA-R: 5'-GTGAGAAGTGTTGACAGGAA-3';

[0122] The ADRB1 primer set includes the following primers:

[0123] ADRB1-FW1: 5'-CTTCCGCAAGGCCTTCCAAC-3';

[0124] ADRB1-FS1: 5'-ACTTCCGCAAGGCCTTCCATG-3';

[0125] ADRB1-R: 5'-TGGCCCCGACGACATCGTCG-3';

[0126] The CYP2D6 primer set includes the following primers:

[0127] CYP2D6-FW1: 5'-ACGCTGGGCTGCACGCTAAC-3';

[0128] CYP2D6-FS1: 5'-AACGCTGGGCTGCACGCTAGT-3';

[0129] CYP2D6-R: 5'-ACCCACCACCCCATGTTTG-3';

[0130] The AGTR1 primer set includes the following primers:

[0131] AGTR1-FW1: 5'-CACTTCACTACCAAATGACCA-3';

[0132] AGTR1-FS1: 5'-CACTTCACTACCAAATGATC...

Embodiment 3

[0149] A primer composition for detecting hypertension drug-related genes includes NPPA primer set, ADRB1 primer set, CYP2D6 primer set, AGTR1 primer set, CYP2C9 primer set, CYP3A5 primer set and GNB3 primer set;

[0150] The NPPA primer set includes the following primers:

[0151] NPPA-FW: 5'-CTGTGTTCCTCTTTGCAGTAGT-3';

[0152] NPPA-FS1: 5'-TGTGTTCCTCTTTGCAGTATC-3';

[0153] NPPA-R: 5'-GTGAGAAGTGTTGACAGGAA-3';

[0154] The ADRB1 primer set includes the following primers:

[0155] ADRB1-FW1: 5'-CTTCCGCAAGGCCTTCCAAC-3';

[0156] ADRB1-FS1: 5'-ACTTCCGCAAGGCCTTCCATG-3';

[0157] ADRB1-R: 5'-TGGCCCCGACGACATCGTCG-3';

[0158] The CYP2D6 primer set includes the following primers:

[0159] CYP2D6-FW1: 5'-ACGCTGGGCTGCACGCTAAC-3';

[0160] CYP2D6-FS1: 5'-AACGCTGGGCTGCACGCTAGT-3';

[0161] CYP2D6-R: 5'-ACCCACCACCCCATGTTTG-3';

[0162] The AGTR1 primer set includes the following primers:

[0163] AGTR1-FW1: 5'-CACTTCACTACCAAATGACCA-3';

[0164] AGTR1-FS1: 5'-CACTTCACTACCAAATGA...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a primer composition and a kit for detecting hypertension medicine related genes. The primer composition comprises an NPPA primer group, an ADRB1 primer group, a CYP2D6 primergroup, an AGTR1 primer group, a CYP2C9 primer group, a CYP3A5 primer group and a GNB3 primer group, and the kit comprises the primer composition. Compared with the prior art, a kit is acquired and can rapidly, sensitively, simply and conveniently detect gene polymorphisms of hypertension medicine related genes (NPPA, ADRB1, CYP2D6, AGTR1, CYP2C9, CYP3A5 and GNB3) by combining an ARMS technique and an SYBR dye, the kit comprises a specific ARMS detection primer, an internal control primer and a PCR reaction solution, the ARMS detection primer is designed, an Scorpions probe replaces with the SYBR dye, so that detection cost is greatly reduced, the primer composition is more suitable for detection NPPA, ADRB1, CYP2D6, AGTR1, CYP2C9, CYP3A5 and GNB3 gene polymorphisms of Chinese patients, and the primer composition has the advantages of rapidness in detection, high sensitivity, high specificity, simple method and accurate result and suitable for popularization and application.

Description

technical field [0001] The invention belongs to the technical field of gene detection, and relates to a primer composition and a kit for detecting genes related to hypertension medication. Background technique [0002] The antihypertensive diuretics began in 1948. Since the advent of chlorothiazide in 1957 for more than 30 years, thiazide diuretics, mainly hydrochlorothiazide, have been one of the main forces of antihypertensive drugs. Whether it is used alone or in combination with other antihypertensive drugs, it has a clear effect. The results of large-scale international clinical trials over the past few decades have further confirmed its position in antihypertensive treatment. Several European and American hypertension treatment principles committees have recommended that diuretics be the drug of choice for uncomplicated hypertensive patients. In recent years, the listing of the new diuretic indapamide (Shoubishan, indapmide) has improved the status of diuretics in th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883C12N15/11
CPCC12Q1/6883C12Q2600/106C12Q2600/156C12Q2600/166
Inventor 田晓丽
Owner 宁波美丽人生医学检验所有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com