Tissue culture seedling hardening-seedling treatment and tissue culture seedling rooting method of malus ornamental crabapple

Abstract

The invention discloses a tissue culture seedling hardening-seedling treatment and tissue culture seedling rooting method of malus ornamental crabapple. The method comprises the following steps: underan aseptic condition, after pruning subcultured tissue culture seedlings of the malus ornamental crabapple on an aseptic operation table, applying rooting glue, inserting into nutrient matrix soil which is sterilized in a moisturizing culture hole, putting into a tissue culture bottle together with the moisturizing culture hole, adding a moisturizing agent to the moisturizing culture hole, performing aseptic and moisturizing culture, after culturing for a predetermined period of time in a given environment, directly removing the moisturizing culture hole, and moving the tissue culture seedlings together with a soil ball into a flower pot to be cultured in a normal indoor light environment. Through the method of transplanting with the soil ball, zero damage to roots during a transplantingprocess is fully ensured, at the same time, a rooting process is directly carried out in the nutrient matrix soil, thereby simplifying a hardening-seedling process in transplanting, improving the adaptability of the tissue culture seedlings to a soil environment in the late transplanting, and greatly improving the survival rate of the transplanting from the original 50% to 90% or over 90%.

Description

technical field

[0001] The invention relates to the field of ornamental crabapple cultivation, in particular to a method for hardening the ornamental crabapple seedlings of the genus Malus and rooting the tissue-cultured seedlings. Background technique

[0002] Ornamental crabapple is a plant of the genus Malus Mill. in the family Rosaceae. Its leaves, flowers and fruits have various color types, bright colors, persistent winter fruits (some varieties), compact tree shape, and strong stress resistance. It is an important resource in fruit trees with both ornamental value and economical value.

[0003] For a long time, the propagation of Malus plants mainly used the method of grafting. Due to the limitation of rootstock cultivation quantity, time, quality and grafting technology, the industrial production of apple seedlings has been restricted. With the rise of tissue culture technology, the industrial production of apple seedlings has become possible. However, the tissue ...