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Pig sperm culture medium and preparation method thereof

A technology of culture medium and pig sperm, applied in the field of culture medium, can solve the problems of high cost and complicated operation, and achieve the effect of low cost

Inactive Publication Date: 2018-09-28
广州伯瑞丁生物新技术开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing porcine sperm culture medium and its preparation method are complex in operation and high in cost

Method used

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Embodiment Construction

[0009] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below.

[0010] The pig sperm culture medium of the embodiment of the present invention comprises the following ingredients: anhydrous glucose: 18-20g, trisodium citrate 6-7g, tris6-7g, EDTA 7-8g, sodium bicarbonate 1-2g, CMC 0.1-1g, anti Binder 0.1-1g, potassium chloride 0.1-1g, citric acid 3-4g, cysteine ​​0.1-1g, GHS 0.01-0.1g, chymotrypsin 0.01-0.05g, melatonin 0.01-0.05g, lysozyme 0.01-0.1g, povidone-iodine 0.01-0.05g.

[0011] Including the following ingredients: anhydrous glucose: 18.5g, trisodium citrate 6.9g, tris6.5g, EDTA 7.5g, sodium bicarbonate 1.3g, CMC 0.4g, anticaking agent 0.6g, potassium chloride 0.5g, citric acid 3.5g, cysteine ​​0.15g, GHS0.03g, chymotrypsin 0.02g, melatonin 0.03g, lysozyme 0.05g, povidone-iodine 0.02g.

[0012] A method for preparing a porcine sperm culture medium, comprising th...

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PUM

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Abstract

The invention discloses a pig sperm culture medium and a preparation method thereof. The pig sperm culture medium comprises the following components of 18-20g of anhydrous dextrose, 6-7g of trisodiumcitrate, 6-7g of tris, 7-8g of EDTA, 1-2g of sodium bicarbonate, 0.1-1g of CMC, 0.1-1g of an anti-caking agent, 0.1-1g of potassium chloride, 3-4g of citric acid, 0.1-1g of cysteine, 0.01-0.1g of GHS,0.01-0.05g of chymotrypsin, 0.01-0.05g of melatonin, 0.01-0.1g of lysozyme, and 0.01-0.05g of povidone-iodine. According to the pig sperm culture medium and the preparation method thereof, operationand manufacturing are easy; first, 1000ml of distilled water for three times of distillation is prepared, 46g of the pig sperm culture medium is placed in the distilled water, a magnetic stirrer is used for enabling the culture medium to be dissolved fully, and standing is performed for 1-2 hours to obtain the pig sperm culture medium; and the costs of the materials are low.

Description

technical field [0001] The invention relates to the culture medium field, in particular to a pig sperm culture medium and a preparation method thereof. Background technique [0002] The existing porcine sperm culture medium and its preparation method are complex in operation and high in cost. Contents of the invention [0003] The technical problem to be solved by the embodiments of the present invention is to provide a porcine sperm culture medium with easy operation and a preparation method thereof. [0004] The pig sperm medium comprises the following components: anhydrous glucose: 18-20g, trisodium citrate 6-7g, tris6-7g, EDTA 7-8g, sodium bicarbonate 1-2g, CMC 0.1-1g, anticoagulant 0.1-1g potassium chloride, 0.1-1g potassium chloride, 3-4g citric acid, 0.1-1g cysteine, 0.01-0.1g GHS, 0.01-0.05g chymotrypsin, 0.01-0.05g melatonin, 0.01 lysozyme -0.1g, povidone-iodine 0.01-0.05g. [0005] Including the following ingredients: anhydrous glucose: 18.5g, trisodium citr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/076
CPCC12N5/061C12N2500/12C12N2500/30C12N2500/32C12N2500/34C12N2500/60C12N2501/30C12N2501/73C12N2501/734
Inventor 王前
Owner 广州伯瑞丁生物新技术开发有限公司
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