Device and method for in-vitro preservation and culturing of blood vessel

Abstract

The invention discloses a device and a method for in-vitro preservation and culturing of a blood vessel, and belongs to the field of biomedical engineering. The device for the preservation and culturing of the blood vessel consists of a blood vessel culture cavity, a circulating system pressure adjuster, a blood vessel deformation adjuster, a pump, a damping adjuster, a liquid storage chamber, anda computer control system, wherein the blood vessel culture cavity, the circulating system pressure adjuster, the pump, the damping adjuster and the liquid storage chamber are connected by pipelinesto form a fluid circulating system; the blood vessel deformation adjuster is used for controlling the deformation of the blood vessel in the blood vessel culture cavity; the circulating system pressure adjuster, the blood vessel deformation adjuster and the pump are controlled by the computer control system; expect the computer control system, the whole device can be placed into a CO2 (carbon dioxide) culture box to perform blood vessel preservation and culture. The device has the advantage that the function of the physical environment on the blood vessel in vivo can be simulated, and the functions of flow rate, pressure, deformation and temperature are applied to the blood vessel or the simulated blood vessel, so that the blood vessel can be dynamically preserved and stored in vitro for alonger time under the bionic simulating state.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, discloses an in vitro preservation and cultivation device for blood vessels and a method thereof, and provides an experimental device and technical scheme for bionic preservation and cultivation of blood vessels in an in vitro simulated human physiological or non-physiological environment. Background technique [0002] It has been possible to preserve and cultivate cells in vitro, but it is quite difficult to cultivate and preserve tissues in vitro, and it is even more difficult to preserve and cultivate tissues in vitro and maintain their physiological characteristics as much as possible. [0003] Cells in a static culture environment are different from those in a dynamic environment that simulates physiology in terms of morphology and gene expression. Only in a dynamic environment that is closer to a physiological environment can it be possible for tissues or organs preserved or cultured in...

AI Technical Summary

Problems solved by technology

[0002] It has been possible to preserve and cultivate cells in vitro, but it is quite difficult to cultivate and preserve tissues in vitro, and it is even more difficult to preserve and cultivate tissues in vitro and maintain their physiological characteristics as much as possible

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