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Construction of a ligand-gated diagnosis and treatment system and its application in real-time tumor response

A gated and systematic technology, applied in the fields of biotechnology and pharmacy, can solve the problems of increasing the difficulty of drug loading, increasing the complexity of synthesis, and reducing clinical application, and achieves reliable and reliable diagnosis, timely and effective treatment, and enhanced simplicity. Effect

Active Publication Date: 2022-07-08
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the optical transparency of commonly used mesoporous silica nanoparticles, the bound fluorescent probes can only be quenched by further surface or pore modification, which not only increases the synthesis complexity and reduces the potential of clinical application, It also increases the difficulty of drug loading (combination of diagnosis and treatment)

Method used

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  • Construction of a ligand-gated diagnosis and treatment system and its application in real-time tumor response
  • Construction of a ligand-gated diagnosis and treatment system and its application in real-time tumor response
  • Construction of a ligand-gated diagnosis and treatment system and its application in real-time tumor response

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Experimental program
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Effect test

Embodiment 1

[0093] The mesoporous carbon nanospheres were treated with a mixture of sulfuric acid and nitric acid (volume ratio of 2:1), sonicated for 2 hours, stirred at 50°C for 2 hours, centrifuged, washed with water, and dried to obtain oxidized mesoporous carbon nanospheres OMCN. OMCN (400 μg / mL) was mixed with Dox (20 μg / mL), stirred at 300 rpm for 6 h, and then added P 0 (2μmol / L) stirred for 1h to obtain Dox-OMCN-P 0 Ligand-gated diagnosis and treatment system, stored at 4°C for later use.

Embodiment 2

[0095] The mesoporous carbon nanospheres were treated with a mixture of sulfuric acid and nitric acid (volume ratio of 3:1), sonicated for 2 hours, stirred at 50°C for 2 hours, centrifuged, washed with water, and dried to obtain oxidized mesoporous carbon nanospheres OMCN. OMCN (400 μg / mL) was mixed with Dox (20 μg / mL), stirred at 300 rpm for 6 h, and then added P 0 (2μmol / L) stirred for 1h to obtain Dox-OMCN-P 0 Ligand-gated diagnosis and treatment system, stored at 4°C for later use.

Embodiment 3

[0097] The mesoporous carbon nanospheres were treated with a mixture of sulfuric acid and nitric acid (4:1 by volume), sonicated for 2 hours, stirred at 50°C for 2 hours, centrifuged, washed with water, and dried to obtain oxidized mesoporous carbon nanospheres OMCN. OMCN (400 μg / mL) was mixed with Dox (20 μg / mL), stirred at 300 rpm for 6 h, and then added P 0 (2μmol / L) stirred for 1h to obtain Dox-OMCN-P 0 Ligand-gated diagnosis and treatment system, stored at 4°C for later use.

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PUM

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Abstract

The invention belongs to the fields of biotechnology and pharmaceutical technology, and relates to a preparation method of an efficient and sensitive ligand-gated diagnosis and treatment system based on OMCN. The invention can realize multiple tumor multiplex in vivo and in vitro by specifically binding MUC1 which is highly expressed on the surface of malignant tumors. Diagnosis and treatment, the OMCN-based ligand-gated diagnosis and treatment system of the present invention has a low detection limit, and can be applied to the detection and treatment of MUC1 molecules, tumor cells and living tumor tissues at different levels, ensuring true and reliable diagnosis and efficient treatment targeting . The invention provides an economical, practical and simple preparation technology of a ligand-gated diagnosis and treatment system based on OMCN, which can realize multiple diagnosis and treatment of tumors in vivo and in vitro.

Description

technical field [0001] The invention belongs to the fields of biotechnology and pharmaceutical technology, relates to a drug-carrying system, and in particular relates to a construction method of a ligand-gated diagnosis and treatment system and its application in real-time tumor response. Background technique [0002] Data show that in recent years, although people have invested a lot of scientific research in the treatment of this major disease, the mortality rate of cancer patients has not been significantly improved. At present, the treatment of tumors in clinical practice is still limited by the mode of separation of diagnosis and treatment. Surveys show that defects such as false positive / negative diagnosis, late diagnosis, tumor metastasis, and off-target side effects still exist. In fact, targeted controllable / on-demand drug delivery based on real-time diagnosis is an ideal tumor treatment mode, which can achieve early and efficient diagnosis of tumors, deliver drugs...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/533G01N33/543G01N21/64
CPCG01N21/6428G01N33/533G01N33/54346G01N33/57484G01N2021/6439G01N2021/6432
Inventor 黄容琴王义李诚意
Owner FUDAN UNIV
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