Oxidized mesoporous carbon nanosphere aptasensor, and preparation method and application thereof

Abstract

The invention relates to an oxidized mesoporous carbon nanosphere aptasensor and a preparation method thereof, and application of the aptasensor to cancer detection, belonging to the field of analytical chemistry. According to the invention, an oxidized mesoporous carbon nanosphere (OMCN) is used as a carrier, and the surface of the OMCN is non-covalently connected with a Cy3-fluorescently-labeled single-stranded DNA probe labeled through Phi-Phi interaction, so the oxidized mesoporous carbon nanosphere aptasensor is prepared. The aptasensor based on OMCN has low detection limit and can be applied to detection of MUC1 molecules, tumor cells, in-vitro tumor tissue and in-vivo tumor tissue at different levels so as to ensure true and credible diagnosis. The invention provides the economical, practical and simple preparation method for the aptasensor based on OMCN, and the prepared aptasensor is applicable to multiple in-vivo and in-vitro diagnosis of tumors.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and relates to an aptamer sensor, in particular to an oxidized mesoporous carbon nanosphere aptamer sensor, its preparation method and its application in cancer detection. Background technique [0002] It has been reported that the incidence and mortality of cancer are increasing day by day, and it has become a major disease that threatens human health. Therefore, it is urgent to develop effective tumor diagnostic technologies, including detection and imaging. At present, in the practice of tumor diagnosis, there are mainly different biochemical detection or imaging techniques, such as enzyme-linked immunosorbent assay (ELISA), hematoxylin-eosin staining (H&E staining), computer X-ray tomography (CT) , Magnetic Resonance Imaging (MRI), etc. are used for diagnosis at different tissue levels. Practice has shown that the detection and diagnosis techniques described are complex and difficult, and m...

AI Technical Summary

Problems solved by technology

At present, in the practice of tumor diagnosis, there are mainly different biochemical detection or imaging techniques, such as enzyme-linked immunosorbent assay (ELISA), hematoxylin-eosin staining (H&E staining), computer X-ray tomography (CT) , magnetic resonance imaging (MRI), etc., are used for diagnosis at different tissue levels. Practice shows that the detection and diagnosis techniques described are complex and difficult, and most of them lack specificity and are prone to false positive results

In recent years, biosensors based on fluorescence quenching-recovery have been considered as a highly sensitive and reliable tool for tumor detection. However, most fluorescent biosensors are easy to detect tumor markers such as nucleic acids, peptides or proteins in liquid tissues, but are rarely used Therefore, it is an important research direction in this technical field to develop new fluorescence quenching materials and prepare biosensors with high specificity for multi-level tumor detection.

[0003] It is known that carbon nanomaterials, including one-dimensional carbon nanotubes and two-dimensional graphene oxide, have special functions, such as excellent fluorescence quenching ability through non-covalent π-π interaction with fluorescently labeled single-stranded DNA and exposure to The quantitative fluorescence recovery ability after the target has been developed and applied to the preparation of fluorescent "Turn-on" biosensors; however, it is still mainly used for single detection of liquid tissues in vitro, mainly due to the toxicity of carbon nanotubes or graphene oxide Larger, easy to aggregate, and functional groups mainly exist at the edge of the interface, etc. More importantly, the one-dimensional and two-dimensional structures are not conducive to uniform interaction with solid tumor tissues; in contrast, three-dimensional carbon nanomaterials, such as Mesoporous carbon nanospheres (MCN) have the potential to overcome the defects of the one-dimensional and two-dimensional materials, such as, (1) the three-dimensional spherical structure has uniform mesopores, large specific surface area, large pore volume, and easy passage of surface functional groups Interact with biomacromolecules; (2) Three-dimensional nanospheres have low rotational energy, and after interacting with solid tumors in vivo, the sensing signal is stable and sensitive; (3) MCN has good biocompatibility and has been explored as a Drug carrier for tumor photothermal therapy

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