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Seed breeding method for increasing content of slowly digestible starch in rice with soft glue viscosity

A technology of slowly digesting starch and soft gel consistency, applied in botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of unfavorable blood sugar for diabetics, fast digestion rate after meals, high blood sugar, etc.

Inactive Publication Date: 2018-11-06
RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, rice with soft gel consistency is often accompanied by fast digestion rate and high blood sugar after meals, which is not conducive to the stability of blood sugar in diabetics

Method used

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  • Seed breeding method for increasing content of slowly digestible starch in rice with soft glue viscosity
  • Seed breeding method for increasing content of slowly digestible starch in rice with soft glue viscosity
  • Seed breeding method for increasing content of slowly digestible starch in rice with soft glue viscosity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Breeding process of improved conventional indica rice Zaoxian 14 with high SDS content

[0018] figure 1 Is the use of RM190 mark-assisted selection Wx g3 Schematic diagram of the gene increasing the SDS content of Early Indica 14, the specific steps are as follows:

[0019] Early rice variety Zaoxian 14 with soft gel consistency and high amylose content was selected as the female parent to cross with rice line W04-10-02-07-10-03 with soft gel consistency and high amylose content to obtain F 1 Seeds; polymorphism detection of RM190 marker of 2 rice parents, Early Indica 14 and W04-10-02-07-10-03 (CT) n The number of repeats is 17 and 10 respectively, with a difference of 7 repeats, and the polymorphism is good; sowing F 1 and Zaoxian 14, using Zaoxian 14 as the recurrent parent, the hybrid F 1 Backcrossing with Early Indica 14, the backcross generation BC was obtained 1 f 1 seed; sow BC 1 f 1 and Early Indica 14, from BC 1 f 1 From generation to gen...

Embodiment 2

[0020] Example 2: Breeding process of improved conventional japonica rice W328 with high SDS content

[0021] figure 2 Is the use of RM190 mark-assisted selection Wx g3 Schematic diagram of the gene to increase the SDS content of W328, the specific steps are as follows:

[0022] The japonica rice variety W328 with soft gelatin consistency and high amylose content popularized in production was selected as the female parent to cross with the rice line W04-10-02-07-10-03 with soft gelatin consistency and high amylose content to obtain F 1 Seeds; polymorphism detection of RM190 markers of 2 rice parents, W328 and W04-10-02-07-10-03 (CT) n The repeat numbers are 17 and 10 respectively, and polymorphism exists; seeding F 1 and W328, with W328 as the recurrent parent, the hybrid F 1 Backcross with W328, get backcross generation BC 1 f 1 seed; sow BC 1 f 1 and W328, from BC 1 f 1 From generation to generation, select 10 individual plants, use RM190 markers to detect substit...

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Abstract

The invention discloses a seed breeding method for increasing content of slowly digestible starch in rice with soft glue viscosity. The method comprises the following steps of using a rice line with glue viscosity greater than 60mm, content of amylose greater than 24% and carrying of Wxg3 gene as a male parent, and using a rice line with glue viscosity greater than 60mm and content of amylose smaller than 16% as a female parent; hybridizing, backcrossing, and assisting to select by a molecular marker, so as to obtain the rice line of which the content of the slowly digestible starch is higherthan the content of slowly digestible starch in the female parent rice line. The method has the advantages that the rice Wxg3 allelic gene is selected in a molecular marker assisting way to improve the fast digestion characteristic of the rice with soft glue viscosity; compared with the original rice line, the characteristic of soft glue viscosity of the rice in the cultured new rice line is not changed, the content of slowly digestible starch is obviously increased, and the other properties (except content of amylose), such as growth period, agronomic character, yield, resistance and adaptability, are very similar; the original rice line and the cultured new rice line are one pair of near-isogenic lines, so that the cultured new rice line can be directly applied to the original rice popularization areas.

Description

technical field [0001] The invention relates to the technical field of rice molecular breeding, in particular to a breeding method for increasing the content of slow-digestible starch in rice with soft gel consistency. Background technique [0002] With the improvement of people's living standards, the quality of rice and its health care functions are increasingly valued by consumers. Among rice quality indicators, gel consistency is a direct indicator reflecting the softness and hardness of rice. Glue consistency reflects the extensibility and softness of rice glue after cooling, generally represented by the extension length of 4.4% rice glue after cooling, divided into three grades: hard glue consistency, the extension length of rice glue is less than 40mm; medium glue For consistency, the extension length of rice glue is 41-60mm; for soft glue consistency, the extension length of rice glue is higher than 60mm. Generally speaking, the consistency of rice with low amylose...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 滕斌倪大虎张瑛罗志祥
Owner RICE RES ISTITUTE ANHUI ACAD OF AGRI SCI
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