Breeding method of hybrid siniperca chuatsi
A technology for mandarin fish and mandarin fish is applied in the field of seedling cultivation, which can solve the problems of low breeding success rate, serious diseases, poor meat texture and the like
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Embodiment 1
[0016] The breeding method of the hybrid mandarin fish of the present embodiment may further comprise the steps:
[0017] A, the striped mandarin fish and the prickly mouth fish that the selection mature is caught from the wild, two kinds of fishes are put into respectively parent culture pool and cultivate, and culture density is 4 tails / cubic meter, dissolved oxygen 5mg / L; Cultivated at a water temperature of -17°C, the frequency of water circulation and filtration in the cultivation pool is 2-3 times / day, and the frequency of water circulation and filtration in the cultivation pond is 2-3 times / day when the fish is cultivated at a water temperature of 18-20°C ;
[0018] B. After 6 days of cultivation, the two fishes reach the critical point of gonad maturation and inject drugs. The injection volume is 0.1 mg / kg. In vitro fertilization with male fish sperm, during the process of extruding female fish eggs and male fish sperm, the ratio of male fish to female fish is 1:2.
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Embodiment 2
[0021] The breeding method of the hybrid mandarin fish of the present embodiment may further comprise the steps:
[0022] A, select sexually mature spotted mandarin fish and prickly-mouthed fish fished from the wild, and put the two kinds of fish into respective parental culture ponds to cultivate. The culture density is 5 tails / cubic meter, dissolved oxygen 6mg / L, and spotted mandarin fish at 16°C Cultivate at a certain water temperature, the frequency of water circulation and filtration in the cultivation pool is 2 times / day, and the frequency of water circulation and filtration in the cultivation pond is 3 times / day when the fish is cultivated at a water temperature of 19°C;
[0023] B. After cultivating for 6-8 days, the two fishes reach the critical point of gonad maturation and inject drugs, chorionic gonadotropin, the injection volume is 0.2 mg / kg, the number of drug injections is once, estrus after 7 hours, and the mothers are extruded respectively. In vitro fertilizat...
Embodiment 3
[0027] The breeding method of the hybrid mandarin fish of the present embodiment may further comprise the steps:
[0028] A, select sexually mature spotted mandarin fish and prickly-mouthed fish caught from the wild, and put two kinds of fish into respective parent culture ponds to cultivate, the culture density is 6 tails / cubic meter, dissolved oxygen 8mg / L, and spotted mandarin fish at 17°C Cultivate at a certain water temperature, the frequency of water circulation and filtration in the cultivation pool is 3 times / day, and the frequency of water circulation and filtration in the cultivation pond is 3 times / day when the fish is cultivated at a water temperature of 20°C.
[0029] B. After 8 days of cultivation, the two fishes reach the critical point of gonad maturation, and inject drugs, chorionic gonadotropin, the injection volume is 0.3 mg / kg, and the number of drug injections is one time. After 8 hours of estrus, the female fish eggs are respectively extruded In vitro fer...
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