Serum-free culture system for inducing differentiation of multipotent stem cells into neural progenitor cells

Abstract

The invention belongs to the technical fields of stem cell biology and cell culture, and proposes a serum-free culture system for inducing differentiation of multipotent stem cells into neural progenitor cells. The serum-free culture system concretely comprises a serum-free differentiation base culture medium and an additive promoting differentiation of the neural progenitor cells, wherein the serum-free base culture medium comprises the following essential ingredients: a DMEM / F12 culture medium, human serum albumin, L-glutamine, D-glucose, vitamin C, an N2 / B27 cell culture additive and non-essential amino acid; and the additive promoting differentiation of the neural progenitor cells concretely comprises SB431542, dermorphin, fibroblast growth factor-2, retinoic acid and a brain-derived neurotrophic factor. The culture medium provided by the invention can help to increase differentiation of the neural progenitor cells in a cell mixed system and reduce differentiation of the neural progenitor cells into other types of cells, so high-purity neural progenitor cells are finally obtained.

Description

technical field [0001] The invention belongs to the technical fields of stem cell biology and cell culture, and in particular relates to a serum-free culture system for promoting the differentiation of induced pluripotent stem cells to neural progenitor cells. Background technique [0002] Neurodegenerative diseases are a kind of diseases with slow onset, progressive development and poor prognosis. So far, there is no effective cure method. Common clinical neurodegenerative diseases include Parkinson's disease, amyotrophic lateral sclerosis, spinal muscular atrophy, and Alzheimer's disease. Although these diseases have different etiologies, they all have different parts of the central nervous system and different degrees of neuron loss and dysfunction in pathology. Replacing lost neurons and restoring their function is a new approach to treating these diseases. Studies in recent years have found that stem cells cultured in vitro can not only proliferate and differentiate, ...

AI Technical Summary

Problems solved by technology

[0004] Most of the medium for pluripotent stem cell culture and differentiation into nerve cells contains fetal bovine serum components. The components of serum are unknown and complex. Due to the large differences in batches of serum products from different manufacturers, the repeatability of the experiment is extremely low.

In addition, serum may also carry pathogens and other pathogenic factors, which greatly reduces the application value and safety of differentiated nerve cells in drug screening testing or cell therapy.

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