A kind of dye method digital quantitative PCR kit and its application and use method

Abstract

The invention discloses a dye method digital quantitative PCR kit and its application and usage method. Including the application of the circular RNA hsa_circ_0051778 copy number as a detection indicator in the differentiation kit between lung adenocarcinoma and tuberculous pleurisy, according to the copy number of circular RNA hsa_circ_0051778 in tuberculous pleurisy is higher than that in lung adenocarcinoma; Among them, the kit includes circular RNA hsa_circ_0051778-specific primers, positive control, negative control, PCR amplification premix for dye-based digital PCR and sterile deionized water; through sampling, PCR amplification system preparation, amplification reaction and identification Steps to use this kit. Therefore, it can accurately and quickly identify lung adenocarcinoma and tuberculous pleurisy. The detection results show that the sensitivity of this detection tool can reach more than 90%, which is higher than the single method currently used clinically.

Description

technical field [0001] The invention relates to a dye method digital quantitative PCR kit and its application and use method, in particular to a dye method digital quantitative PCR kit based on circular RNA hsa_circ_0051778 as a detection index and its application and use method, belonging to medical detection tools . Background technique [0002] Lung adenocarcinoma and tuberculous pleurisy are both clinically common respiratory diseases, and some clinical manifestations of the two diseases are similar, but their treatment options and prognosis are significantly different. Therefore, accurate diagnosis of these two diseases is of great importance to patients. Prognosis is extremely important. [0003] Pleural effusion refers to the abnormal increase of pleural fluid caused by certain diseases affecting the pleura. Lung adenocarcinoma and tuberculous pleurisy are common causes of pleural effusion. At present, the diagnosis can be made by detecting Mycobacterium tuberculosi...

AI Technical Summary

Problems solved by technology

Lung adenocarcinoma and tuberculous pleurisy are common causes of pleural effusion. At present, the diagnosis can be made by detecting Mycobacterium tuberculosis or tumor cells in pleural effusion, but the diagnosis will be confirmed clinically through medical history, blood test, pleural effusion biochemical test, and erythrocyte sedimentation rate test. , tuberculosis antibody detection, cell chromosome detection, tumor antigen detection, etc. to make differential diagnosis between the two, the identification process is cumbersome, time-consuming, and increases the economic burden of patients, so the positive detection rate in actual clinical work is low

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