Rapid double staining solution for freehand sections of main stems of tobaccos

A technique of double staining and free-hand sectioning, which is applied in the field of dyeing of plant free-hand sectioning, can solve the problems of non-staining, time-consuming, and complicated elution steps.

Active Publication Date: 2018-11-30
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dyeing effect of safranin-aniline blue and safranin-fast green double dyeing method is better, but the two dyes of these two double dyeing methods need to be dyed step by step. Takes about 30 minutes or longer
Other staining solutions for freehand sections, such as phloroglucinol staining fast, can stain lignified cells, but other structural cells in the stem are not stained; fast green staining is fast, the cell structure in the stem is clear, but lignified vessels and non-lignified Catheters are indistinguishable
Therefore, there is no technology in the art to combine the two staining solutions with complementary staining effects for double staining

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for rapid double dyeing of tobacco main stems by freehand sectioning includes the following steps:

[0023] 1) Prepare fast double staining solution and eluent; among them, the fast double staining solution is prepared from 0.5g of phloroglucinol, 0.0375g of fast green, 50mL of concentrated hydrochloric acid, and 50mL of distilled water; the eluent is hydrochloric acid with pH 0.25 Solution

[0024] 2) Hold the main stem of the tobacco plant to be sliced ​​in the left hand, and hold the double-sided blade in the right hand. Dip a small amount of distilled water with the knife edge inward, and slice the cross section in a uniform motion; place the cut slices in a plate filled with distilled water ;

[0025] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to suck up the excess distilled water;

[0026] 3) Drop 1-2 drops of fast double staining solution on the surface of the sample, and wait for abou...

Embodiment 2

[0029] A method for rapid double dyeing of tobacco main stems by freehand sectioning includes the following steps:

[0030] 1) Prepare fast double staining solution and eluent; among them, fast double staining solution is prepared from 0.37g of phloroglucinol, 0.036g of fast green, 50mL of concentrated hydrochloric acid, and 50mL of distilled water; the eluent is hydrochloric acid with pH 0.25 Solution

[0031] 2) Hold the main stem of the tobacco plant to be sliced ​​with the left hand, and hold the double-sided blade with the right hand. Dip a small amount of distilled water with the knife edge inward, and slice the cross section in a uniform motion; Place the cut slices in a plate filled with distilled water Inside;

[0032] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to suck up the excess distilled water;

[0033] 3) Drop 1-2 drops of fast double staining solution on the surface of the sample, and wait fo...

Embodiment 3

[0036] A method for rapid double dyeing of tobacco main stems by freehand sectioning includes the following steps:

[0037] 1) Prepare fast double staining solution and eluent; among them, the fast double staining solution is prepared from 0.375g of phloroglucinol, 0.0375g of fast green, 50mL of concentrated hydrochloric acid, and 50mL of distilled water; the eluent is hydrochloric acid with pH 0.25 Solution

[0038] 2) Hold the main stem of the tobacco plant to be sliced ​​in the left hand, and hold the double-sided blade in the right hand. Dip a small amount of distilled water with the knife edge inward, and slice the longitudinal section in a uniform motion; Place the cut slices in a plate filled with distilled water Inside;

[0039] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to suck up the excess distilled water;

[0040] 3) Drop 1-2 drops of fast double staining solution on the surface of the sample, an...

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PUM

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Abstract

The invention relates to a staining technology of freehand sections of plants, and in particular relates to rapid double staining solution for freehand sections of main stems of tobaccos, and a staining method. The invention aims to provide the rapid staining method for increasing the staining efficiency of the freehand sections of the main stems of the tobaccos. The adopted technical scheme is asfollows: a rapid double staining method for the freehand sections of the main stems of the tobaccos comprises the steps of: (1), preparing rapid double staining solution and eluant; (2), putting a freehand section sample of the main stems of tobaccos on a glass slide, and removing excess distilled water by absorption through a straw; (3), dropping 1-2 drops of rapid double staining solution ontothe surface of the sample, and waiting for about one minute till the xylem of the sample has macroscopic red; and (4), removing the rapid double staining solution by absorption through the straw, dropping 1-2 drops of eluant onto the surface of the sample, decolouring till the sample becomes reseda and the xylem of the sample becomes purple, and then, performing microscopy shooting. According to the method in the invention, double staining of the freehand sections of the main stems of the tobaccos can be completed for about one minute.

Description

Technical field [0001] The invention relates to a dyeing technique for plant freehand sectioning, in particular to a rapid double staining solution and a dyeing method for the freehand section of tobacco main stems. Background technique [0002] The double staining method of freehand section is a common method for observing various anatomical structures of plants. Safranin-aniline blue and saffron-fast green double dyeing methods have better dyeing effects, but the two dyes of these two double dyeing methods need to be dyed step by step. The elution step is cumbersome and takes a long time to complete one dyeing and decolorization. It takes about 30 minutes or even longer. Other freehand section staining solutions, such as phloroglucinol staining fast, can stain lignified cells, but other structural cells in the stem are not stained; fast green staining, the cell structure in the stem is clear, but the lignified ducts are not lignified The difference between the catheters is no...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 许娜石屹张彦杜沙沙
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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