Rapid double staining solution for freehand sections of main stems of tobaccos
A technique of double staining and free-hand sectioning, which is applied in the field of dyeing of plant free-hand sectioning, can solve the problems of non-staining, time-consuming, and complicated elution steps.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0022] A method for rapid double dyeing of tobacco main stems by freehand sectioning includes the following steps:
[0023] 1) Prepare fast double staining solution and eluent; among them, the fast double staining solution is prepared from 0.5g of phloroglucinol, 0.0375g of fast green, 50mL of concentrated hydrochloric acid, and 50mL of distilled water; the eluent is hydrochloric acid with pH 0.25 Solution
[0024] 2) Hold the main stem of the tobacco plant to be sliced in the left hand, and hold the double-sided blade in the right hand. Dip a small amount of distilled water with the knife edge inward, and slice the cross section in a uniform motion; place the cut slices in a plate filled with distilled water ;
[0025] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to suck up the excess distilled water;
[0026] 3) Drop 1-2 drops of fast double staining solution on the surface of the sample, and wait for abou...
Embodiment 2
[0029] A method for rapid double dyeing of tobacco main stems by freehand sectioning includes the following steps:
[0030] 1) Prepare fast double staining solution and eluent; among them, fast double staining solution is prepared from 0.37g of phloroglucinol, 0.036g of fast green, 50mL of concentrated hydrochloric acid, and 50mL of distilled water; the eluent is hydrochloric acid with pH 0.25 Solution
[0031] 2) Hold the main stem of the tobacco plant to be sliced with the left hand, and hold the double-sided blade with the right hand. Dip a small amount of distilled water with the knife edge inward, and slice the cross section in a uniform motion; Place the cut slices in a plate filled with distilled water Inside;
[0032] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to suck up the excess distilled water;
[0033] 3) Drop 1-2 drops of fast double staining solution on the surface of the sample, and wait fo...
Embodiment 3
[0036] A method for rapid double dyeing of tobacco main stems by freehand sectioning includes the following steps:
[0037] 1) Prepare fast double staining solution and eluent; among them, the fast double staining solution is prepared from 0.375g of phloroglucinol, 0.0375g of fast green, 50mL of concentrated hydrochloric acid, and 50mL of distilled water; the eluent is hydrochloric acid with pH 0.25 Solution
[0038] 2) Hold the main stem of the tobacco plant to be sliced in the left hand, and hold the double-sided blade in the right hand. Dip a small amount of distilled water with the knife edge inward, and slice the longitudinal section in a uniform motion; Place the cut slices in a plate filled with distilled water Inside;
[0039] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to suck up the excess distilled water;
[0040] 3) Drop 1-2 drops of fast double staining solution on the surface of the sample, an...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com