A Quick Double Staining Solution for Freehand Slicing of Tobacco Main Stems
A double-dyeing and free-hand sectioning technique, applied in the field of plant free-hand sectioning, can solve the problems of cumbersome elution steps, non-staining, and long time-consuming
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Embodiment 1
[0022] A rapid double staining method for freehand sections of tobacco main stems, comprising the following steps:
[0023] 1) Prepare rapid double staining solution and eluent; wherein, the rapid double staining solution is prepared from 0.5 g of phloroglucinol, 0.0375 g of fast green, 50 mL of concentrated hydrochloric acid, and 50 mL of distilled water; the eluent is hydrochloric acid with a pH value of 0.25 solution;
[0024] 2) Hold the main stem of the tobacco plant to be sliced with the left hand, and the double-sided blade with the right hand, dip a small amount of distilled water, with the edge of the knife facing inward, and make cross-section slices with uniform movements; place the cut slices in a plate filled with distilled water ;
[0025] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to absorb excess distilled water;
[0026] 3) Drop 1-2 drops of rapid double staining solution on the s...
Embodiment 2
[0029] A rapid double staining method for freehand sections of tobacco main stems, comprising the following steps:
[0030] 1) Prepare rapid double staining solution and eluent; wherein, the rapid double staining solution is prepared from 0.37 g of phloroglucinol, 0.036 g of fast green, 50 mL of concentrated hydrochloric acid, and 50 mL of distilled water; the eluent is hydrochloric acid with a pH value of 0.25 solution;
[0031] 2) Hold the main stem of the tobacco plant to be sliced with the left hand, and the double-sided blade with the right hand, dip a small amount of distilled water, with the knife edge facing inward, and make cross-section slices with uniform movements; place the cut slices on a plate filled with distilled water Inside;
[0032] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to absorb excess distilled water;
[0033] 3) Drop 1-2 drops of fast double staining solution on the sur...
Embodiment 3
[0036] A rapid double staining method for freehand sections of tobacco main stems, comprising the following steps:
[0037] 1) Prepare rapid double staining solution and eluent; wherein, the rapid double staining solution is prepared from 0.375 g of phloroglucinol, 0.0375 g of fast green, 50 mL of concentrated hydrochloric acid, and 50 mL of distilled water; the eluent is hydrochloric acid with a pH value of 0.25 solution;
[0038] 2) Hold the main stem of the tobacco plant to be sliced with the left hand, and the double-sided blade with the right hand, dip a small amount of distilled water with the edge of the knife facing inward, and make longitudinal slices with uniform movements; place the cut slices on a plate filled with distilled water Inside;
[0039] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to absorb excess distilled water;
[0040] 3) Drop 1-2 drops of rapid double staining solution on...
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