A Quick Double Staining Solution for Freehand Slicing of Tobacco Main Stems

A double-dyeing and free-hand sectioning technique, applied in the field of plant free-hand sectioning, can solve the problems of cumbersome elution steps, non-staining, and long time-consuming

Active Publication Date: 2020-12-01
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dyeing effect of safranin-aniline blue and safranin-fast green double dyeing method is better, but the two dyes of these two double dyeing methods need to be dyed step by step. Takes about 30 minutes or longer
Other staining solutions for freehand sections, such as phloroglucinol staining fast, can stain lignified cells, but other structural cells in the stem are not stained; fast green staining is fast, the cell structure in the stem is clear, but lignified vessels and non-lignified Catheters are indistinguishable
Therefore, there is no technology in the art to combine the two staining solutions with complementary staining effects for double staining

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A rapid double staining method for freehand sections of tobacco main stems, comprising the following steps:

[0023] 1) Prepare rapid double staining solution and eluent; wherein, the rapid double staining solution is prepared from 0.5 g of phloroglucinol, 0.0375 g of fast green, 50 mL of concentrated hydrochloric acid, and 50 mL of distilled water; the eluent is hydrochloric acid with a pH value of 0.25 solution;

[0024] 2) Hold the main stem of the tobacco plant to be sliced ​​with the left hand, and the double-sided blade with the right hand, dip a small amount of distilled water, with the edge of the knife facing inward, and make cross-section slices with uniform movements; place the cut slices in a plate filled with distilled water ;

[0025] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to absorb excess distilled water;

[0026] 3) Drop 1-2 drops of rapid double staining solution on the s...

Embodiment 2

[0029] A rapid double staining method for freehand sections of tobacco main stems, comprising the following steps:

[0030] 1) Prepare rapid double staining solution and eluent; wherein, the rapid double staining solution is prepared from 0.37 g of phloroglucinol, 0.036 g of fast green, 50 mL of concentrated hydrochloric acid, and 50 mL of distilled water; the eluent is hydrochloric acid with a pH value of 0.25 solution;

[0031] 2) Hold the main stem of the tobacco plant to be sliced ​​with the left hand, and the double-sided blade with the right hand, dip a small amount of distilled water, with the knife edge facing inward, and make cross-section slices with uniform movements; place the cut slices on a plate filled with distilled water Inside;

[0032] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to absorb excess distilled water;

[0033] 3) Drop 1-2 drops of fast double staining solution on the sur...

Embodiment 3

[0036] A rapid double staining method for freehand sections of tobacco main stems, comprising the following steps:

[0037] 1) Prepare rapid double staining solution and eluent; wherein, the rapid double staining solution is prepared from 0.375 g of phloroglucinol, 0.0375 g of fast green, 50 mL of concentrated hydrochloric acid, and 50 mL of distilled water; the eluent is hydrochloric acid with a pH value of 0.25 solution;

[0038] 2) Hold the main stem of the tobacco plant to be sliced ​​with the left hand, and the double-sided blade with the right hand, dip a small amount of distilled water with the edge of the knife facing inward, and make longitudinal slices with uniform movements; place the cut slices on a plate filled with distilled water Inside;

[0039] 3) Use tweezers or a brush to remove the sample from the distilled water and place it on a glass slide, and use a straw to absorb excess distilled water;

[0040] 3) Drop 1-2 drops of rapid double staining solution on...

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PUM

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Abstract

The invention relates to a staining technology of freehand sections of plants, and in particular relates to rapid double staining solution for freehand sections of main stems of tobaccos, and a staining method. The invention aims to provide the rapid staining method for increasing the staining efficiency of the freehand sections of the main stems of the tobaccos. The adopted technical scheme is asfollows: a rapid double staining method for the freehand sections of the main stems of the tobaccos comprises the steps of: (1), preparing rapid double staining solution and eluant; (2), putting a freehand section sample of the main stems of tobaccos on a glass slide, and removing excess distilled water by absorption through a straw; (3), dropping 1-2 drops of rapid double staining solution ontothe surface of the sample, and waiting for about one minute till the xylem of the sample has macroscopic red; and (4), removing the rapid double staining solution by absorption through the straw, dropping 1-2 drops of eluant onto the surface of the sample, decolouring till the sample becomes reseda and the xylem of the sample becomes purple, and then, performing microscopy shooting. According to the method in the invention, double staining of the freehand sections of the main stems of the tobaccos can be completed for about one minute.

Description

technical field [0001] The invention relates to a dyeing technology for freehand slices of plants, in particular to a rapid double dyeing liquid and a dyeing method for freehand slices of tobacco main stems. Background technique [0002] The double-staining method of freehand sections is a common method for observing the anatomical structures of plants. The dyeing effect of safranin-aniline blue and safranin-fast green double dyeing method is better, but the two dyes of these two double dyeing methods need to be dyed step by step. It takes about 30 minutes, or even longer. Other staining solutions for freehand sections, such as phloroglucinol staining fast, can stain lignified cells, but other structural cells in the stem are not stained; fast green staining is fast, the cell structure in the stem is clear, but lignified vessels and non-lignified Catheters are not distinct. Therefore, there is no technology in the art to combine the two staining solutions with complementa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/30
CPCG01N1/30G01N2001/302
Inventor 许娜石屹张彦杜沙沙
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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