Positive charge nanogold coupling antibody wrapped by mesoporous silica as well as preparation method and application thereof

A technology of mesoporous silica and silica, applied in the field of immunology, can solve problems such as low accuracy and complicated operation, and achieve the effects of rapid detection, high catalytic activity, and improved stability

Pending Publication Date: 2020-01-03
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects and deficiencies of the immunoassay method for determining abamectin in the prior art that require secondary antibody treatment, complicated operation, and low accuracy, and provide a positively charged nanometer coated with mesoporous silica. gold conjugated antibody

Method used

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  • Positive charge nanogold coupling antibody wrapped by mesoporous silica as well as preparation method and application thereof
  • Positive charge nanogold coupling antibody wrapped by mesoporous silica as well as preparation method and application thereof
  • Positive charge nanogold coupling antibody wrapped by mesoporous silica as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～3

[0061] Examples 1-3 Positively charged gold nanoparticles wrapped by mesoporous silica

[0062] Embodiments 1-3 provide a series of positively charged gold nanoparticles wrapped by mesoporous silica, as follows.

Embodiment 1

[0064] This embodiment provides a preparation method of positively charged gold nanoparticles wrapped in mesoporous silica, and the preparation method is as follows.

[0065] (1) Synthesis of silica nanoparticles: Dissolve 0.5g of CTAB in 240mL of pure water, add NaOH (1.75mL, 2mol / L) into the CTAB solution, then adjust the temperature of the solution to 80°C and continue stirring while adding 2.5mL TEOS, the mixture was stirred for 3h, the white precipitate was obtained, the solid product was separated by centrifugation, washed several times with deionized water and ethanol, and dried in air.

[0066] (2) Preparation of mesoporous silica: The synthesized silica nanoparticles (0.5 g) were dispersed in ethanol (15 mL) for 30 min by sonication, and then 30 mL of a 1:1 mixture (v:v) was added for deionization water and 1,3,5-trimethylbenzene. The mixture was placed in an autoclave and kept at 140 °C for 24 h without stirring, and the white powder was washed several times with et...

Embodiment 2

[0069] This embodiment provides a preparation method of positively charged gold nanoparticles wrapped in mesoporous silica, and the preparation method is as follows.

[0070] (1) Synthesis of silica nanoparticles: Dissolve 0.5g of CTAB in 240mL of pure water, add NaOH (1.75mL, 2mol / L) into the CTAB solution, then adjust the temperature of the solution to 100°C and continue stirring while adding 2.5mL TEOS, the mixture was stirred for 3h, the white precipitate was obtained, the solid product was separated by centrifugation, washed several times with deionized water and ethanol, and dried in air.

[0071] (2) Preparation of mesoporous silica: The synthesized silica nanoparticles (0.5 g) were dispersed in ethanol (15 mL) for 30 min by sonication, and then 30 mL of a 1:1 mixture (v:v) was added for deionization water and 1,3,5-trimethylbenzene. The mixture was placed in an autoclave and kept at 160 °C for 24 h without stirring, and the white powder was washed several times with e...

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Abstract

The invention relates to a positive charge nanogold coupling antibody wrapped by mesoporous silica as well as a preparation method and application thereof. The positive charge nanogold coupling antibody is obtained by coupling between positive charge nanogold wrapped by the mesoporous silica and an avermectin antibody. According to the positive charge nanogold coupling antibody of the invention, the mesoporous silica is used for wrapping the positive charge nanogold, so that the obtained positive charge nanogold wrapped by the mesoporous silica is stable; in addition, the mesoporous silica wrapping further improves the horseradish peroxidase-like characteristics of the positive charge nanogold, the color of TMB can be developed, and high catalytic activity is achieved; through adoption ofthe positive charge nanogold coupling antibody wrapped by the mesoporous silica, a step of secondary antibody can be skipped to achieve color development of the TMB, and rapid detection of the abamectin is further realized.

Description

technical field [0001] The invention belongs to the technical field of immunology, and in particular relates to a positively charged nano-gold coupled antibody wrapped in mesoporous silicon dioxide, a preparation method and application thereof. Background technique [0002] Abamectin (AVM) is an insecticidal / acaricidal compound derived from the soil bacterium Streptomyces avermitilis. Five AVMs, avermectin (ABM), ivermectin (IVM), eprinomectin (EPR), doramectin (DOR) and emamectin (EMA), are widely used Used in agriculture and farm animals for the treatment of broad-spectrum parasitic diseases. AVM is characterized by the presence of a sugar molecule substituent at position 13 and a sec-butyl or isopropyl group at position 25. AVM acts on invertebrates and vertebrates by acting on the γ-aminobutyric acid (GABA) receptors in the central nervous system of animals to play an insecticidal role. Due to the lipophilic nature of AVM and the long-term persistence of its animal re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535G01N33/541G01N33/543
CPCG01N33/535G01N33/541G01N33/54346
Inventor 赵肃清张春国何绮怡崔锡平沈定钟颖颖
Owner GUANGDONG UNIV OF TECH
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