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Method for analyzing and judging grafting success rate of carya cathayensis sarg through proteome succinylation

A technology of succinylation and proteomics, applied in the field of plant cultivation, can solve the problems of grafting effect prediction and intervention, grafting process cannot be monitored, and the cycle is long

Pending Publication Date: 2018-11-30
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the grafting cycle of this anvil and general grafting technology is long, and the success rate of grafting is low, especially the grafting process cannot be monitored, making it difficult to predict and intervene in the grafting effect

Method used

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  • Method for analyzing and judging grafting success rate of carya cathayensis sarg through proteome succinylation
  • Method for analyzing and judging grafting success rate of carya cathayensis sarg through proteome succinylation
  • Method for analyzing and judging grafting success rate of carya cathayensis sarg through proteome succinylation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Types and sites of succinylated proteins in the survival process of pecan grafting

[0039] The hickory grafted joint was used as the experimental material of this study. The experimental material was derived from the grafted seedlings of the 2-year-old rootstock and the 1-year-old scion. The sampling site was the joint between the rootstock and the scion. For 0 days and 7 days after grafting, the cells on the joint surface of the rootstock and the scion were scraped with a razor blade during sampling, the proteins were extracted, and sequenced.

[0040] A total of 259 succinylation sites were identified in 202 proteins by HPLC combined with tandem mass spectrometry. These proteins are involved in a variety of biological processes and have a wide range of representation. At the same time, the differential expression analysis of pecan succinylated proteins was completed, and 1.2 times was set as the threshold for up-regulated proteins, and 0.83 was set as the ...

Embodiment 2

[0051] Example 2 Annotation results of pecan succinylated proteins

[0052] We categorized all pecan succinylated proteins with functionally enriched properties according to quantitative expression levels. All quantified pecan succinylated proteins were divided into 4 categories: up-regulated by more than 1.5 times (Q1), up-regulated by 1.3-1.5 times (Q2), down-regulated by more than 1.5 times (Q4) and down-regulated by 1.3-1.5 times (Q3 ). After statistical analysis, the results showed that a total of 7 pecan succinylated proteins belonged to the Q1 category, 12 pecan succinylated proteins belonged to the Q2 category, 17 pecan succinylated proteins belonged to the Q3 category, and 14 pecan succinylated proteins belonged to the Q3 category. Succinylated proteins fall into the Q4 category.

[0053] In order to further study the biological function of pecan succinylated protein, protein function annotations were performed on the obtained pecan succinylated protein, which inclu...

Embodiment 3

[0059] Example 3 Identification of Pecan Succinylation Site-Specific Amino Acids

[0060] As identified by the Motif-X software, a total of 259 sites of 202 proteins are succinylated, and the motifs of succinylated specific binding sites include 4 types, namely:······KP···· ·········K·E································K····················································································· ····, their matching coefficients (Motif Score) are 16.00, 16.00, 17.50 and 9.98 respectively. The present invention first discovers multiple motifs in pecan succinylated proteins, which have not been reported in other species before. Different species have different succinylation motifs, and studying the differences in motifs can help to distinguish the differences in succinylation between species. The results of our examples show that the motifs of the amino acid sites of succinylation in pecan are unique to this species, and they are related to the biological characteristic...

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Abstract

The invention provides a method for analyzing and judging the grafting success rate of carya cathayensis sarg through proteome succinylation. A carya cathayensis sarg grafting joint part contains 202succinylation proteins. Go classification of the succinylation proteins belongs to the metabolic process, the cell process, the single-species process, stimulation response, protein localization, protein binding, catalytic activity and oxidation-reduction activity correspondingly. The proteins are located in chloroplast, cytoplasm, cell nucleuses, mitochondria, cytoplasmic membranes, vacuole membranes, intercellular substances or endoplasmic reticula. It is discovered that after grafting of the carya cathayensis sarg, the 202 proteins and 259 loci can be subjected to succinylation for the first time, and the succinylation proteins involved in various biological processes can be used as an identification standard for successful grafting of the carya cathayensis sarg.

Description

technical field [0001] The invention belongs to the field of plant cultivation, and in particular relates to a method for judging the success rate of pecan grafting by using proteome succinylation analysis. Background technique [0002] Hickory (Carya cathayensis Sarg.), also known as small walnut, mountain crab (Zhejiang), walnut, wild sumac (Anhui), is a deciduous tree belonging to the genus Hickory of the family Juglandaceae, suitable for growing in the sparse forests of foothills or in humus Rich valley, China is one of the origin. The adult hickory tree is 10-20 meters high and 30-60 cm in diameter at breast height; the bark is smooth and off-white; the branchlets are thin and thin, and the new branches are densely covered with orange-yellow glands that are shield-shaped, and later the glands gradually become sparse; the 1-year-old branches are purple-gray , the upper end is often covered with sparse pubescence; the lenticels are round and sparse. The fruit of pecan h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/00G01N30/02
CPCG01N33/0098G01N30/02G01N2030/027
Inventor 郑炳松袁虎威闫道良何漪沈晨佳陈娟娟杨影徐栋斌
Owner ZHEJIANG FORESTRY UNIVERSITY
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