An offline supercritical extraction-supercritical chromatography-mass spectrometry combined detection method for polyphenols

A supercritical chromatography and supercritical technology, which is applied in the field of offline-SFE-SFC-MS/MS detection of polyphenols, can solve the problem that the rapid detection method of polyphenols needs to be studied, garlic polyphenols are unstable, and The processing process is cumbersome and other problems, to achieve the effect of fast detection speed, high degree of automation, and small sample volume

Active Publication Date: 2018-12-07
CHINESE ACAD OF INSPECTION & QUARANTINE
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Problems solved by technology

Garlic polyphenolic compounds are unstable, and the pretreatment process of garlic samples in some literatures is very cumbersome and time-consuming, so it is easy to decompose when using traditional solvent extraction methods
In recent years, the analysis method for the content of polyphenolic compounds in food is mainly high performance liquid chromatography, which has low sensitivity and poor accuracy.
[0003] Therefore, rapid detection methods for polyphenols in food and pharmaceuticals need to be studied

Method used

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  • An offline supercritical extraction-supercritical chromatography-mass spectrometry combined detection method for polyphenols
  • An offline supercritical extraction-supercritical chromatography-mass spectrometry combined detection method for polyphenols
  • An offline supercritical extraction-supercritical chromatography-mass spectrometry combined detection method for polyphenols

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Embodiment 1

[0059] Utilize the offline-SFE-SFC-MS / MS detection method of the embodiment of the present invention to detect polyphenols in garlic, the specific method is as follows:

[0060] (1) Materials:

[0061] Shandong Cangshan garlic was purchased from Beijing Hualian Shopping Center; nine kinds of polyphenol standard products were purchased from Sigma Corporation of the United States.

[0062] (2) Method:

[0063] 1. Offline supercritical extraction (offline-SFE):

[0064] Take 0.5g of the garlic raw material to be tested and pulverize it, and mix it evenly with 0.5g of the dehydrating agent "MIYAZAKIHydro-Protect" for Shimadzu's SFE, fill it into the SFE extraction tank and add it to the extraction tank. Cross-optimized polyphenol extraction process, wherein the response surface design is shown in Table 1, the proportion of methanol in the extraction solvent (by volume fraction) is 30%-50%, the extraction time is 1-9min, and the extraction temperature is 30-50°C. Garlic polyphen...

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Abstract

An offline supercritical extraction-supercritical chromatography-mass spectrometry combined detection method for polyphenols is disclosed. The method includes after a food to be detected is mixed witha dewatering agent, bringing the mixture into contact with an extracting liquid to perform supercritical extraction to obtain a liquid to be detected; and detecting the liquid to be detected throughsupercritical chromatography-mass spectrometry so as to qualitatively and quantitatively analyze polyphenols in the food, medicines, and the like. The polyphenols in the food are extracted through supercritical extraction, the method is simple, high in degree of automation, and low in sample amount in need, and oxidation and decomposition of polyphenols can be effectively avoided. In addition, thesupercritical chromatography-mass spectrometry is adopted for detection so that the detection speed is high and sensitivity and accuracy are high.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to an offline-SFE-SFC-MS / MS detection method for polyphenols. Background technique [0002] Polyphenols widely exist in a variety of foods, for example, there are many polyphenols in garlic. Garlic polyphenolic compounds are unstable, and the pretreatment process of garlic samples in some literatures is very cumbersome and time-consuming, so the traditional solvent extraction method is prone to decomposition. In recent years, the analysis method of polyphenolic compounds in food is mainly high performance liquid chromatography, which has low sensitivity and poor accuracy. [0003] Therefore, a rapid detection method for polyphenols in food and medicine needs to be studied. Contents of the invention [0004] The present invention aims to solve at least one of the technical problems existing in the prior art. For this reason, an object of the present invention is to propose a ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 张峰刘建刘通国伟杨敏莉
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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