Transporting fluid for human umbilical cord mesenchymal stem cells
A technology for transporting fluid and stem cells, applied in the field of stem cells, can solve the problems of reducing the number of living cells and being unsuitable for long-distance transportation, and achieve the effect of improving the survival rate
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[0014] 1. Experimental materials
[0015] Choose fresh umbilical cords from healthy puerperas, sign the informed consent form, and agree to be used in scientific research experiments.
[0016] Human umbilical cord mesenchymal stem cell culture medium was purchased from Saiye Company; fetal bovine serum was purchased from American Thermo Company; DMEM medium was purchased from American Gibco Company; The culture medium was purchased from Shanghai Raas Blood Products Co., Ltd.; the stem cell osteogenic differentiation medium was purchased from PromoCell; the neural differentiation medium: 20 μg / L epidermal growth factor and 20 μg / L were added to the DMEM / F12 medium containing 2% B27 Formulated with Basic Fibroblast Growth Factor of L.
[0017] 2. Experimental method
[0018] 1. Isolation and culture of human umbilical cord mesenchymal stem cells (hUCMSCs)
[0019] Cut the umbilical cord and wash it several times with PBS, take out Huatong jelly, cut it into pieces, digest it ...
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