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Transporting fluid for human umbilical cord mesenchymal stem cells

A technology for transporting fluid and stem cells, applied in the field of stem cells, can solve the problems of reducing the number of living cells and being unsuitable for long-distance transportation, and achieve the effect of improving the survival rate

Active Publication Date: 2018-12-18
江苏赛亿细胞技术研究院有限公司
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Problems solved by technology

However, the number of viable cells of the three types of cells (human amniotic fluid, umbilical cord, and placenta-derived mesenchymal stem cells) decreased significantly after being placed in the cell transport solution at room temperature for 72 hours, which is not suitable for long-distance transport

Method used

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Embodiment Construction

[0014] 1. Experimental materials

[0015] Choose fresh umbilical cords from healthy puerperas, sign the informed consent form, and agree to be used in scientific research experiments.

[0016] Human umbilical cord mesenchymal stem cell culture medium was purchased from Saiye Company; fetal bovine serum was purchased from American Thermo Company; DMEM medium was purchased from American Gibco Company; The culture medium was purchased from Shanghai Raas Blood Products Co., Ltd.; the stem cell osteogenic differentiation medium was purchased from PromoCell; the neural differentiation medium: 20 μg / L epidermal growth factor and 20 μg / L were added to the DMEM / F12 medium containing 2% B27 Formulated with Basic Fibroblast Growth Factor of L.

[0017] 2. Experimental method

[0018] 1. Isolation and culture of human umbilical cord mesenchymal stem cells (hUCMSCs)

[0019] Cut the umbilical cord and wash it several times with PBS, take out Huatong jelly, cut it into pieces, digest it ...

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Abstract

The invention relates to a transporting fluid for human umbilical cord mesenchymal stem cells. According to the invention, heterophyllin B with a concentration of 5 [mu]M is added on the basis of theprior art, so the shortcoming that conventional transporting fluids is obviously reduced in cell viability after suspension for 72 h can be effectively overcome. The transporting fluid of the invention is prepared by uniformly mixing, by mass, 30% of a trehalose solution (a PBS solution of trehalose) with a concentration of 0.2%, 25% of a red blood cell storage solution, 5% of a heparin calcium solution (a PBS solution of heparin calcium) with a concentration of 0.2% and 40% of human albumin injection at room temperature, and further comprises heterophyllin B with a concentration of 5 [mu]M. The transporting fluid of the invention can improve the survival rate of human umbilical cord mesenchymal stem cells having been transported for 72 hours, and does not affect the multi-directional differentiation ability of the human umbilical cord mesenchymal stem cells.

Description

technical field [0001] The invention relates to the field of stem cells, in particular to a transport liquid for human umbilical cord mesenchymal stem cells. Background technique [0002] Stem cells are a class of cells with self-renewal and multilineage differentiation capabilities. It is because of its regenerative potential and plasticity that it is widely used in regenerative medicine and stem cell research. Stem cells offer hope for many diseases that currently lack effective treatments, including stroke, Huntington's disease, Alzheimer's disease and Parkinson's disease. [0003] Mesenchymal stem cells are multipotent stromal cells isolated from adult and fetal tissues, defined as adherent fibroblast-like cells. Mesenchymal stem cells have intrinsic homing ability, can migrate to damaged tissues, and actively participate in tissue repair. Although mesenchymal stem cells are pluripotent, their self-renewal ability gradually decreases leading to stagnation of cell divi...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 卢珊珊郑桂纯
Owner 江苏赛亿细胞技术研究院有限公司
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