Low-cost cell culture apparatus

A cell culture, low-cost technology, applied in the field of low-cost cell culture devices, can solve the problems that the number of cells cannot meet the clinical needs, the clinical application of stem cell therapy is difficult, and the influence of cell growth is large, so as to shorten the growth configuration cycle and improve Cell culture efficiency and effect, effect of optimizing culture parameters

Inactive Publication Date: 2018-12-21
曾小敏
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Both cell therapy and tissue engineering require a considerable number of stem cells. However, the source of stem cells is limited. The current planar culture operations such as ordinary multi-well plates and square flasks are complicated. The intermittent changes in the growth microenvironment have a great impact on cell growth. The number of harvested cells Far from meeting the clinical needs
At the same time, directly adding cytokine combinations to the culture system will greatly increase the cost of culture, making it difficult to promote stem cell therapy to clinical application

Method used

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  • Low-cost cell culture apparatus
  • Low-cost cell culture apparatus
  • Low-cost cell culture apparatus

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Experimental program
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Effect test

Embodiment 1

[0028] Such as Figure 1-7 As shown, the low-cost cell culture device includes a perfusion bottle 1, and the perfusion bottle 1 is connected to the reactor 12 through the input pipe 6, and the reactor 12 is connected to the peristaltic pump 8 through the first communication pipe 7, and the peristaltic pump 8 is connected through the second communication tube 7. The pipe 9 is connected with the perfusion tank 14, and the perfusion tank 14 is connected with the waste liquid bottle 11 through the output pipe 10. The reactor 12 and the perfusion tank 14 are all provided with a filter membrane 13, and the input pipe 6, the first connecting pipe 7, and the second connecting pipe Both the pipe 9 and the output pipe 10 are provided with shock absorbers 5 . In the present invention, the feeder layer cells and the cells to be cultured are respectively cultured in the reactor 12 and the perfusion tank 14, and the specific pore size of the filter membrane 13 provided can be controlled to ...

Embodiment 2

[0038] When the low-cost cell culture device of the present invention is actually used: the medium for stem cell culture is packed in the perfusion bottle 1, and the medium in the perfusion bottle 1 flows into the reactor 12 through the peristaltic pump 10, and is filtered in the reactor 12. The feeder cells under the membrane 13 provide nutrition, and the embryonic stem cells are inoculated in the perfusion tank 14. The feeder cells in the reactor 12 secrete cytokines and culture fluid to form a conditioned medium, which is input into the perfusion tank 14 for the cultivation of embryonic stem cells. After the growth, the leftover culture medium is input into the waste liquid bottle 11 for collection.

[0039] In the initial stage of cell culture, the magnetic field strength of the magnetic field generator 16 is controlled to be relatively small, so that the magnetic spheres 15 are suspended in the lower part of the reactor 12 or the perfusion tank 14, so that the higher densi...

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Abstract

The invention discloses a low-cost cell culture apparatus, which comprises a perfusion bottle, the perfusion bottle is connected to a reactor through an input tube, the reactor is connected to a peristaltic pump through a first communicating tube, the peristaltic pump is connected to a perfusion tank through a second communicating tube, the perfusion tank is connected to a waste liquid bottle through an output tube, filter membranes are arranged in both the reactor and the perfusion tank, and shock absorbers are respectively arranged on the input tube, the first communicating tube, the secondcommunicating tube and the output tube. The low-cost cell culture apparatus can independently culture stem cells, ensures the purity of stem cells, and effectively shortens stem cell culture time, andthe culture cost is low.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a low-cost cell culture device. Background technique [0002] Stem cells are considered ideal seed cells for cell therapy and tissue engineering because of their self-renewal and multi-directional differentiation potential. Both cell therapy and tissue engineering require a considerable number of stem cells. However, the source of stem cells is limited. The current planar culture operations such as ordinary multi-well plates and square flasks are complicated. The intermittent changes in the growth microenvironment have a great impact on cell growth. The number of harvested cells It is far from reaching the clinical needs. At the same time, directly adding a combination of cytokines to the culture system will greatly increase the cost of culture, making it difficult to promote stem cell therapy to clinical application. Therefore, there is an urgent need for a de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/42C12M1/38C12M1/00
CPCC12M23/02C12M41/12C12M41/26C12M41/48C12M45/07
Inventor 曾小敏
Owner 曾小敏
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