A high-throughput tumor-targeted drug concentration screening microfluidic device

Abstract

The invention provides a high-throughput tumor-targeted drug concentration screening microfluidic device, which is composed of a cell sampling layer, a pneumatic film layer and a cell control layer. The cell sampling layer includes a multi-cell sampling channel and a valve control channel, which can realize simultaneous sampling of various cells. The cell control layer has a "two-stage parallel" structure, the first-stage parallel structure is a concentration gradient generator module, and the second-stage parallel structure is a cell culture module. Among them, the concentration gradient generator module can realize the uniform mixing of granular tumor-targeted drugs and generate drug concentrations in various ratios; the multi-cell culture module can provide a dynamic, uniform and stable fluid flow microenvironment for cell culture, and achieve Environment-independent culture of multiple cell types for targeted drug action. The microfluidic device provides a new technical platform for high-throughput cell culture, tumor-targeted drug screening and cell-drug experiments.

Description

Technical field [0001] The present invention belongs to the application of microfluidic chips and its biomedicine, drug screening, etc., and more particularly to a high-throughput tumor target concentration screening microfluidic device. [0002] technical background [0003] Cells are based on biological structures, functions and life activities, and cell research is of great significance for understanding organisms, revealing life activities, drug screening, diagnosis of diseases. Cell culture is the premise of cell research, and cell culture has a key effect on cell value, differentiation and expression. The fluid environment in most tissue cells in the organism is a gap flow, which has the characteristics of stable flow stable and small shear force. Therefore, it is of great significance to construct cellular microenvironment during cell extraction, on the one hand, the cell culture provides dynamic, stable, uniform, low-shear fluid flow micro-environment; on the other hand, c...

AI Technical Summary

Problems solved by technology

Among them, the most typical one is the "Christmas tree" configuration concentration gradient generator, which can form a wide linear range of gradients, but its concentration gradient ratio is fixed to be linear and cannot be adjusted according to demand.

In the existing cell culture unit based on microfluidics, in the cell culture area, there is a large amount of fluid in direct contact with the cells, forming a large shear force, thereby affecting or even destroying the cells.

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