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Molecular marker used for detecting zhongmai 895 fusarium head blight resistance QTL and use method

A kind of scab, DNA molecule technology

Active Publication Date: 2018-12-21
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, except for Fhb1, the breeding applications of other genes are less reported
In addition, there are still problems in the genetic research of wheat head blight resistance, such as little research on production and application varieties, and lack of available molecular markers for breeding.

Method used

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  • Molecular marker used for detecting zhongmai 895 fusarium head blight resistance QTL and use method
  • Molecular marker used for detecting zhongmai 895 fusarium head blight resistance QTL and use method
  • Molecular marker used for detecting zhongmai 895 fusarium head blight resistance QTL and use method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 3

[0063] The 157 wheat materials shown in Example 3 are recorded in the literature: Zhu Zhanwang, Xu Dengan, Cheng Shunhe, et al. Identification and traceability of the scab resistance gene Fhb1 in Chinese wheat varieties [J]. Acta Crops, 2018, 44(4 ):473–482. Publicly available from Institute of Crop Science, Chinese Academy of Agricultural Sciences.

Embodiment 1

[0064] Example 1. Acquisition of Scab Resistance QTL QFhb.caas-5AL and Linkage Markers

[0065] Test materials: Zhongmai 895, Yangmai 16, and DH populations (including 174 families) constructed by using Yangmai 16 as the female parent and Zhongmai 895 as the male parent.

[0066] Field scab resistance identification: The test was carried out in Wuhan during 2016-2017 and 2017-2018. The experiment adopts completely randomized block design, 2-row block, row length 1m, row spacing 0.25m, 2 repetitions. Mark 10 ears that are blooming and have the same shape, and spray the conidia suspension (5 × 10 5 per mL), the pathogenic strain was Huanggang No. 1; from the heading stage to the end of the investigation, a microcomputer timing mist device was used to humidify to promote the onset of disease. 20 days after inoculation, the number of diseased panicles, the number of spikelets per panicle and the number of diseased spikelets were investigated, and the FHB index was calculated. A...

Embodiment 2

[0070] Embodiment 2, establishment of Fhb-5AL-InDel labeling method

[0071] 1. Genome-specific primer design

[0072] The genetic position of AX-89588684 on the linkage map 5A is 120.62cM, and the physical position on the Chinese spring reference genome (IWGSCRefSeq v1.0) is 570.0Mb, genetically with the markers AX-111258351 and AX-111001072 on both sides of QFhb.caas-5AL Both location and physical proximity are available for marker development. Using the flanking sequence (sequence 5) of SNP AX-89588684, compare it in the EnsemblPlants database (http: / / plants.ensembl.org), obtain its homologous sequence, and design its 5A chromosome-specific primer P8684 (sequence 1 and sequence 2) , synthesized by Beijing Qingke Xinye Biotechnology Co., Ltd.

[0073] P8684F: 5'-TTCCCCTCCCCTACATATGAGATG-3' (SEQ ID NO: 1);

[0074] P8684R: 5'-CGATAAGGCAAAAACGAGGTGAC-3' (SEQ ID NO: 2).

[0075] 2. Establishment of detection method

[0076] Test materials: Zhongmai 895, Yangmai 16, and DH ...

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Abstract

The invention discloses a molecular marker used for detecting a zhongmai 895 fusarium head blight resistance QTL and a use method. According to the molecular marker used for detecting the zhongmai 895fusarium head blight resistance QTL, by conducting fusarium head blight resistance QTL (quantitative trait locus) positioning on a DH (double haploid) group built through production application varieties yangmai 16 and zhongmai 895, the fusarium head blight resistance QTL QFhb.caas-5AL is found on a 5A chromosome long arm, an LOD value is 2.8-9.4, explained phenotypic variation is 2.4%-9.0%, markers on the two sides are AX-111258351 and AX-111001072, and a disease resistance allele comes from the zhongmai 895. On the basis, an InDel (insertion deletion) marker Fhb-5AL-InDel in close linkage with the QFhb.caas-5AL is developed, and a good tool is provided for fusarium head blight resistance breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker for detecting the QTL of scab resistance in Zhongmai 895 and a method for using it. Background technique [0002] Fusarium head blight (FHB) caused by Fusarium graminearum is a widespread fungal disease that seriously affects wheat yield and quality. More importantly, the susceptible grains contain mycotoxins, such as deoxynivalenol (DON), which endanger the health of humans and animals and affect food and feeding. [0003] The middle and lower reaches of the Yangtze River and the Northeast wheat region in my country are areas where wheat scab frequently occurs and reoccurs. In recent years, affected by global climate change and the popularity of straw returning under the wheat-corn rotation system, the disease has shown a trend of expansion and aggravation, and has become a common disease in the Huanghuai wheat region. At present, the average annual occurrence a...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 郝元峰朱展望陈延平徐小婷徐登安夏先春何中虎
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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