A pretreatment device for enriching fecal exfoliated cells with immunomagnetic beads in the department of oncology

Abstract

The invention relates to a pretreatment device for enriching fecal exfoliated cells by immunomagnetic beads in oncology. The device comprises a treatment cylinder, treatment accessories, an automaticwashing device and a filtering system; a first external thread and a second external thread are arranged at the two ends of the treatment cylinder respectively; the treatment accessories include a conical bearing funnel, an upper sealing cover, a lower sealing bottom and a primary filtering cylinder; the automatic washing device comprises a flushing pipe, a rotating cylinder, a rotating cylinder base, a driving gear and a speed reducing motor; and the filtering system comprises a first liquid discharging pipe, a peristaltic pump, a filter and a second liquid discharging pipe. A pretreatment bottle performs sampling for a whole sample; through primary filtering, liquid in feces is filtered out; then the whole sample of the solid feces is treated; and the treated liquid is subjected to uniform mixing and multistage filtering to obtain qualified cell suspension liquid containing the exfoliated cells, so that the problem of pollution is not caused, the psychology of an operator also is notinfluenced, and the compliance problem of an inspector is avoided.

Description

technical field [0001] The invention relates to the technical field of oncology, in particular to a pretreatment device for enriching feces exfoliated cells with immune magnetic beads in the oncology department. Background technique [0002] Colorectal cancer refers to cancers in the rectum, sigmoid colon, cecum, and colon, among which rectal cancer and colon cancer are the most common and one of the most common malignant tumors of the digestive tract. Colorectal cancer has the characteristics of high malignancy and early metastasis. Therefore, the key to the treatment of colorectal cancer lies in early diagnosis, because the intestinal epithelial tissue has been undergoing cell renewal very rapidly, at a rate of about 1% per hour. The vigorously growing tumor epithelial cells have a faster renewal rate, and exfoliated cells can provide Detecting intestinal tumors, polyps, ulcers and other abnormalities provides a variety of detection methods, such as gene mutation detection...

AI Technical Summary

Problems solved by technology

But it has the following disadvantages: (1) The operator needs to take a sample with a small spoon and put it in the sample collector. During the sampling process, it is not clean, and it is not easy to ensure the cleanliness of the laboratory. The redundant samples are disposed of separately, and it is easy to pollute the environment. It is easy to cause psychological pressure on the operator, and the operator in this step is a nurse or the family member of the patient, so sampling is prone to deviation; (2) sampling is random, which affects the overall evaluation; (3) the bottom of the sample collector is sealed with tin foil, It is easy to be punctured during the transfer process, which affects the sample delivery. The tin foil is punctured during the sample processing process. Large pieces of tin foil are in the sample. During the processing process, it is easy to block the metal filter and affect the discharge of the washing liquid; (4) Other The filtration precision is small, and two layers of filters with different pore sizes are stacked together, which will easily cause the filter to be blocked, which is not conducive to the outflow of the washed cell suspension

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