A kind of preparation method of drug-loaded hemostatic sponge and prepared drug-loaded hemostatic sponge

Abstract

The invention discloses a preparation method of drug-loading hemostatic sponge. The preparation method comprises the following steps: (1) preparing lignocelluloses sponge; and (2) loading the lignocelluloses sponge with chitosan and a hemostatic drug in a spraying or immersion manner, and freeze drying to obtain the drug-loading hemostatic sponge. A preparation method of the lignocelluloses spongecomprises the following steps: (1) freezing an alkali urea solution, forming icy slurry, adding lignocelluloses into the icy slurry, and obtaining a lignocelluloses solution; (2) adding a surfactantinto the lignocelluloses solution, stirring, and forming a foam body; (3) freezing the foam body into foam icy slurry, adding a pore forming agent into the foam icy slurry, injection molding, freezing, aging, and obtaining gel; and (4) soaking to remove the surfactant and the pore forming agent in the gel, freeze shaping, freeze drying, and obtaining the lignocelluloses sponge. By adopting the drug-loading hemostatic sponge prepared by the invention, by virtue of the synergistic effect of the chitosan and hemostatic drug, the blood on the surface of a wound can be rapidly absorbed, the blood coagulation of the wound can be effectively promoted, and the bleeding can be rapidly stopped.

Description

technical field

[0001] The invention belongs to the field of medical polymer materials, and in particular relates to a preparation method of a drug-loaded hemostatic sponge, and also relates to a drug-loaded hemostatic sponge product prepared by the method. Background technique

[0002] Blood is an important carrier that transports oxygen to the body. When the human body loses too much blood due to injury, the organs and cells will malfunction and fail, which will affect life safety. Uncontrolled bleeding has always been the leading cause of casualties. Although protected by personal protective equipment such as bulletproof vests and helmets, in modern warfare, bleeding is still the main cause of death in battlefield trauma, especially when the trauma occurs in the joints of limbs such as armpits, groin, neck, etc., these parts cannot be used with tourniquets Or manual compression for hemostasis can easily lead to hemorrhagic shock or even death. Therefore, timely and eff...

Examples

Embodiment 1

[0047] Embodiment 1: Discussion experiment of determining the amount of lignocellulose

[0048] Preparation of Lignocellulose Sponge

[0049] 1), take sodium hydroxide and urea, and prepare an alkali urea solution with an alkali concentration of 7wt% and a urea concentration of 12wt%; place the alkali urea solution in a cold trap for quick freezing for 10 minutes to form an ice slurry containing suspended ice crystal particles Add lignocellulose to the alkali urea solution in ice slurry state while stirring to obtain lignocellulose solution;

[0050] 2), put the lignocellulose solution prepared in step 1) into a cold trap for 10 minutes to quickly freeze to form an ice layer, then add a porogen while stirring, place it into a mold and place it in a freezer at -18°C for 48 hours to obtain gel;

[0051] 3) Put the gel prepared in step 2) into water to soak and wash for 2 days, change the water 5-6 times during soaking to remove the porogen, then freeze at -18°C for 24 hours to...

Embodiment 2

[0058] Embodiment two: preparation lignocellulose sponge

[0059] The preparation method of lignocellulose sponge, comprises the following steps:

[0060] 1), take sodium hydroxide and urea, and prepare an alkali urea solution with an alkali concentration of 7wt% and a urea concentration of 12wt%; place the alkali urea solution in a cold trap for quick freezing for 10 minutes to form an ice slurry containing suspended ice crystal particles Add lignocellulose to the alkali urea solution in ice slurry state while stirring to obtain lignocellulose solution;

[0061] 2), adding a surfactant to the lignocellulose solution prepared in step 1), stirring quickly to make the system form a stable foam;

[0062] 3) Put the foam prepared in step 2) into a cold trap to freeze for 10 minutes to form a foam ice slurry, add porogen to the foam ice slurry while stirring, and freeze and age at -18°C for 48 hours after injection molding , to get a gel;

[0063] 4) Soak and wash the gel prepar...

Embodiment 3

[0084] Embodiment three: loading chitosan and hemostatic drug on lignocellulose sponge

[0085] Load chitosan and hemostatic drugs on lignocellulose sponge by impregnation method, the operation steps are as follows:

[0086] a, take the acid-soluble chitosan whose degree of deacetylation is greater than 90%, dissolve the acid-soluble chitosan in 0.1mol / / L dilute acetic acid, and prepare a chitosan solution; add hemostatic drugs to the chitosan solution , stir and disperse evenly to obtain a chitosan-hemostatic drug composite solution; wherein, the mass fraction of chitosan in the chitosan solution is 0.1wt% to 2wt%, and the hemostatic drug in the chitosan-hemostatic drug composite solution The mass fraction of the drug is 0.1wt% to 1wt%;

[0087] b. Soak the lignocellulose sponge prepared in Examples 2-4 in the chitosan-hemostatic drug composite solution for 2 hours, take it out, freeze it at -18° C., and then freeze-dry it to obtain a drug-loaded hemostatic sponge.

[0088]...

technical field

[0001] The invention belongs to the field of medical polymer materials, and in particular relates to a preparation method of a drug-loaded hemostatic sponge, and also relates to a drug-loaded hemostatic sponge product prepared by the method. Background technique

[0002] Blood is an important carrier that transports oxygen to the body. When the human body loses too much blood due to injury, the organs and cells will malfunction and fail, which will affect life safety. Uncontrolled bleeding has always been the leading cause of casualties. Although protected by personal protective equipment such as bulletproof vests and helmets, in modern warfare, bleeding is still the main cause of death in battlefield trauma, especially when the trauma occurs in the joints of limbs such as armpits, groin, neck, etc., these parts cannot be used with tourniquets Or manual compression for hemostasis can easily lead to hemorrhagic shock or even death. Therefore, timely and eff...

Examples

Embodiment 1

[0047] Embodiment 1: Discussion experiment of determining the amount of lignocellulose

[0048] Preparation of Lignocellulose Sponge

[0049] 1), take sodium hydroxide and urea, and prepare an alkali urea solution with an alkali concentration of 7wt% and a urea concentration of 12wt%; place the alkali urea solution in a cold trap for quick freezing for 10 minutes to form an ice slurry containing suspended ice crystal particles Add lignocellulose to the alkali urea solution in ice slurry state while stirring to obtain lignocellulose solution;

[0050] 2), put the lignocellulose solution prepared in step 1) into a cold trap for 10 minutes to quickly freeze to form an ice layer, then add a porogen while stirring, place it into a mold and place it in a freezer at -18°C for 48 hours to obtain gel;

[0051] 3) Put the gel prepared in step 2) into water to soak and wash for 2 days, change the water 5-6 times during soaking to remove the porogen, then freeze at -18°C for 24 hours to...

Embodiment 2

[0058] Embodiment two: preparation lignocellulose sponge

[0059] The preparation method of lignocellulose sponge, comprises the following steps:

[0060] 1), take sodium hydroxide and urea, and prepare an alkali urea solution with an alkali concentration of 7wt% and a urea concentration of 12wt%; place the alkali urea solution in a cold trap for quick freezing for 10 minutes to form an ice slurry containing suspended ice crystal particles Add lignocellulose to the alkali urea solution in ice slurry state while stirring to obtain lignocellulose solution;

[0061] 2), adding a surfactant to the lignocellulose solution prepared in step 1), stirring quickly to make the system form a stable foam;

[0062] 3) Put the foam prepared in step 2) into a cold trap to freeze for 10 minutes to form a foam ice slurry, add porogen to the foam ice slurry while stirring, and freeze and age at -18°C for 48 hours after injection molding , to get a gel;

[0063] 4) Soak and wash the gel prepar...

Embodiment 3

[0084] Embodiment three: loading chitosan and hemostatic drug on lignocellulose sponge

[0085] Load chitosan and hemostatic drugs on lignocellulose sponge by impregnation method, the operation steps are as follows:

[0086] a, take the acid-soluble chitosan whose degree of deacetylation is greater than 90%, dissolve the acid-soluble chitosan in 0.1mol / / L dilute acetic acid, and prepare a chitosan solution; add hemostatic drugs to the chitosan solution , stir and disperse evenly to obtain a chitosan-hemostatic drug composite solution; wherein, the mass fraction of chitosan in the chitosan solution is 0.1wt% to 2wt%, and the hemostatic drug in the chitosan-hemostatic drug composite solution The mass fraction of the drug is 0.1wt% to 1wt%;

[0087] b. Soak the lignocellulose sponge prepared in Examples 2-4 in the chitosan-hemostatic drug composite solution for 2 hours, take it out, freeze it at -18° C., and then freeze-dry it to obtain a drug-loaded hemostatic sponge.

[0088]...

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