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Method for detecting vitamin A, vitamin D and vitamin E simultaneously

A detection method and vitamin technology, which can be applied to measurement devices, instruments, scientific instruments, etc., can solve the problem of not being able to accurately measure fat-soluble vitamin A at the same time, and achieve the effects of saving detection time, simplifying detection steps, and optimizing detection parameters.

Inactive Publication Date: 2019-01-04
INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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  • Abstract
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Problems solved by technology

[0005] The purpose of the present invention is to solve the defect that fat-soluble vitamins A, D, and E cannot be accurately measured simultaneously in the prior art, and then provide a method for simultaneously measuring vitamins A, D, and E with high recovery rate

Method used

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  • Method for detecting vitamin A, vitamin D and vitamin E simultaneously
  • Method for detecting vitamin A, vitamin D and vitamin E simultaneously
  • Method for detecting vitamin A, vitamin D and vitamin E simultaneously

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Embodiment Construction

[0056] The technical solutions of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Apparently, the described embodiments are some of the embodiments of the present invention, but not all of them. In addition, the technical features involved in the different embodiments of the present invention described below may be combined with each other as long as there is no conflict with each other.

[0057] In the following examples and comparative examples, ultraviolet detectors are used to detect vitamins. Of course, other detectors such as diode array detectors and fluorescence detectors can also be used to detect vitamins. All the examples provided below are for vitamin D 3 Of course, the method provided by the invention can also carry out vitamin D simultaneously 3 with D 2 The mensuration of the standard solution preparation method used in the following examples is as follows:

[0058] Preparation of standard s...

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Abstract

The invention belongs to the technical field of chemical ingredient detection, and particularly relates to a method for detecting multiple kinds of vitamin simultaneously. According to the detecting method, multiple lipid-soluble vitamin samples contained in a sample can be fast and accurately measured simultaneously through a two-dimensional liquid chromatographic analysis method. According to the method, effluent in a specific period of a one-dimensional chromatographic column is injected to a two-dimensional chromatographic column, effluent of the two-dimensional chromatographic column is subjected to vitamin D measurement, the other effluent of the one-dimensional chromatographic column is subjected to measurement of non-vitamin D lipid-soluble vitamin, peakoverlap of vitamin D and other lipid-soluble vitamin can be avoided, and the influence of lipid matter in the sample on the detection accuracy of vitamin D is effectively reduced. According to the detecting method, the normal-phase column purifying process on vitamin D in a traditional method can be omitted, tedious detecting steps are simplified, detection time is saved, and high accuracy can be guaranteed.

Description

technical field [0001] The invention belongs to the technical field of chemical component detection, and in particular relates to a method for simultaneously detecting multiple vitamins. Background technique [0002] Vitamins A, D, and E are fat-soluble vitamins necessary for the body to maintain normal metabolism. Vitamin A (Retinol), also known as retinol, has the functions of promoting body growth, maintaining epidermal integrity and vision; vitamin D mainly includes vitamin D 2 (ergocalciferol) and vitamin D 3 (cholecalciferol), which can promote calcium and phosphorus metabolism and bone formation in mammals; vitamin E is also known as tocopherol, mainly including α-tocopherol, β-tocopherol, γ-tocopherol and δ-tocopherol Among them, α-tocopherol has the highest activity, which has the functions of promoting fertility and anti-aging. Therefore, in the food industry, vitamins A, D, and E are often added, but because these vitamins are relatively unstable, they are very...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/46
CPCG01N30/02G01N30/463
Inventor 牛世祯宋晓东郭波常建军徐向峰李照袁凤琴
Owner INNER MONGOLIA MENGNIU DAIRY IND (GRP) CO LTD
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