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Fluorescent copper nanoparticles, preparation method thereof and application in detecting content of riboflavin and sulfur ions

A technology of nanoparticles and fluorescent copper, which is applied in the direction of measuring devices, nanotechnology, fluorescence/phosphorescence, etc., and can solve the problems of complex sample processing, high cost of raw materials, and expensive instruments

Active Publication Date: 2020-06-23
QINGHAI UNIV FOR NATITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently reported methods for the detection of sulfide ions are: fluorescence, ion chromatography, electrochemiluminescence, thin-layer chromatography, colorimetry, etc. These methods also have certain disadvantages in application, such as expensive instruments and complicated sample processing , difficulty in operation, etc. Among them, the fluorescence method is relatively simple to operate and has high sensitivity. There are many fluorescent probes such as carbon dots combined with noble metals to detect sulfur ions, but the cost of raw materials is high. Therefore, inorganic fluorescent nanoprobes are simple to prepare, Low cost and more and more attention

Method used

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  • Fluorescent copper nanoparticles, preparation method thereof and application in detecting content of riboflavin and sulfur ions
  • Fluorescent copper nanoparticles, preparation method thereof and application in detecting content of riboflavin and sulfur ions
  • Fluorescent copper nanoparticles, preparation method thereof and application in detecting content of riboflavin and sulfur ions

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Experimental program
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preparation example Construction

[0045] 2.1 Preparation of copper nano-solution CuNPs

[0046] Weigh 32 mg of anhydrous copper sulfate, add 2 mL of 0.5 mol / L NaOH solution, add 20 mL of ultrapure water and stir for 2 min, transfer to a rotary evaporator (the rotary evaporator is wrapped in a black bag), turn off the condensation switch, and close the exhaust plug. Add 20mL of 0.1 mol / L ascorbic acid solution, the color of the solution turns egg yellow, rotate at 50°C for 10 minutes, adjust the pH to pH=8-9 with 0.5mol / L NaOH solution, continue to rotate at 50°C for 15 hours, the color of the solution finally changes to Greenish brown, CuNPs formed, stored in a 4°C refrigerator in the dark.

[0047] 2.2 Formation of fluorescence resonance energy

[0048] VB2 was detected fluorescently in PBS buffer solution at pH=7.5 (0.1M) at room temperature. The specific process is as follows: first pipette 25 μl (36.025 mM) CuNPs solution into a quartz cuvette and add 3 mL (0.1M, pH=7.5) of PBS buffer solution, then add ...

Embodiment 1

[0139] 1 Preparation of copper nano-solution CuNPs

[0140] Weigh 32 mg of anhydrous copper sulfate, add 2 mL of 0.5 mol / L NaOH solution, add 20 mL of ultrapure water and stir for 2 min, transfer to a rotary evaporator (the rotary evaporator is wrapped in a black bag), turn off the condensation switch, close the exhaust plug, add 20mL 0.1mol / L ascorbic acid solution, the color of the solution turns egg yellow, rotate at 50°C for 10min, adjust the pH to pH=8 with 0.5mol / L NaOH solution, continue to rotate at 50°C for 15 hours, the color of the solution finally turns greenish brown, CuNPs Formed, protected from light and stored in a 4°C refrigerator.

[0141] 2VB 2 concentration detection

[0142] The oranges are pre-treated by crushing them first, centrifuging them, taking the supernatant, preparing the concentration required for the test, and refrigerating them in the refrigerator for later use.

[0143] Take 25μL (36.025uM) CuNPs solution, add 3mL PBS (pH=7.5) and 300μL of...

Embodiment 2

[0145] 1 Preparation of copper nano-solution CuNPs

[0146] Weigh 30 mg of anhydrous copper sulfate, add 4 mL of 0.1mol / L NaOH solution, add 20 mL of ultrapure water and stir for 2 min, transfer to a rotary evaporator (the rotary evaporator is wrapped in a black bag), turn off the condensation switch, close the exhaust plug, add 20mL 0.06mol / L ascorbic acid solution, the color of the solution turns egg yellow, rotate at 90°C for 10min, adjust the pH to pH=5 with 0.5mol / L NaOH solution, continue to rotate at 90°C for 17 hours, the color of the solution finally turns greenish brown, CuNPs Formed, protected from light and stored in a 4°C refrigerator.

[0147] 2VB 2 concentration detection

[0148] The oranges are pre-treated by crushing them first, centrifuging them, taking the supernatant, preparing the concentration required for the test, and refrigerating them in the refrigerator for later use.

[0149] Take 25μL (36.025uM) CuNPs solution, add 3mL PBS (pH=7.5) and 300μL of...

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Abstract

The invention provides a preparation method of fluorescent copper nanoparticles. Anhydrous copper sulfate serves as a copper source, ascorbic acid is added, and a low-temperature rotary evaporation closed system liquid-phase chemical reduction method is utilized to prepare the fluorescent copper nanoparticles. The invention also provides the fluorescent copper nanoparticles prepared through the method and application of the fluorescent copper nanoparticles in detecting the content of VB2 and sulfur ions. A copper nano-solution with blue fluorescence is synthesized first, copper nanoparticles have strong fluorescence at about 440 nm, riboflavin has strong ultraviolet absorption at about 450 nm, and the copper nanoparticles and riboflavin are almost completely overlapped, so that it is supposed that the resonance energy transfer (FERT) process occurs between the copper nanoparticles and riboflavin, and therefore a fluorescence resonance energy transfer system between the copper nanoparticles and riboflavin is established and can be used for detecting riboflavin. In the copper nanoparticle and riboflavin system, S2- reacts with VB2, so that fluorescence quenching of the copper nanoparticle and riboflavin system happens, and therefore the sulfur ions can be detected.

Description

technical field [0001] The invention relates to a fluorescent copper nano particle, a preparation method thereof and an application in detecting the content of riboflavin and sulfide ions. Background technique [0002] In recent years, fluorescence resonance energy transfer technology has been widely used in the field of biosensing. Fluorescence resonance energy transfer refers to the photophysical process of non-radiative transfer of energy between fluorescent donor groups and acceptors. The interaction of poles occurs between the donor and the acceptor. The condition of fluorescence resonance energy transfer is relatively harsh, that is, when the emission spectrum of the donor overlaps with the absorption spectrum of the acceptor by more than 30%, and the distance between the two is less than 10nm. At a certain excitation wavelength, the emission of the fluorescent donor can be absorbed by the nearby fluorescent acceptor, which leads to shortening of the fluorescence inte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B22F1/00B82Y40/00C09K11/58G01N21/64
CPCB22F9/24B82Y40/00C09K11/58G01N21/6428G01N21/643B22F1/054
Inventor 王欢
Owner QINGHAI UNIV FOR NATITIES
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