The present invention discloses an epoxy resin based on 2,5-furandicarboxylic acid, which is a compound has a structure represented by a formula I, is prepared by directly adopting biological origin2,5-furandicarboxylic acid as a raw material, is a biological-base, green and environmentally friendly product, and has dual effects of petroleum resource saving and environment protection. The present invention further discloses a preparation method for the epoxy resin based on 2,5-furandicarboxylic acid, wherein the preparation method has characteristics of simple preparation, good controllability and easy performing. The present invention further discloses an application of the epoxy resin based on 2,5-furandicarboxylic acid, wherein the epoxy resin based on 2,5-furandicarboxylic acid can be applicable for composite materials, epoxy adhesives, coatings and other fields, wherein the epoxy resin based on 2,5-furandicarboxylic acid can be adopted to prepare cured products containing the epoxy resin based on 2,5-furandicarboxylic acid, and can be adopted as a composite material to provide excellent mechanical property and excellent thermal property. Formula I.