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The lipid-soluble vitamins are often associated with toxicity when taken in large amounts. Vitamin C and the B vitamins are water soluble. When water-soluble vitamins are taken in excess, they are readily excreted in the urine and not usually associated with toxicity.
Method for simultaneously detecting retinoids and liposoluble vitamin metabolic marker groups in biological samples and matching reagents
The application discloses a method for simultaneously detecting retinoids and lipid-soluble vitamin metabolic marker groups in biological samples and matching reagents. The application is based on magnetic bead solid-phase extraction technology, significantly improves sample purification effect, and reduces matrix interference; in combination with a high-aqueous-phase reversed-phase chromatography system and a positive-negative ion switching multi-reaction monitoring mode, simultaneous separation and detection of seven retinoid molecules and a full spectrum of lipid-soluble vitamins are realized. The method has high sensitivity, strong specificity and wide detection range, and for the first time realizes one-stop analysis across species and sample types, thereby providing reliable platform support for clinical and scientific research applications.
Owner:BEIJING TSINGHUA CHANGGUNG HOSPITAL