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Culture medium for pine lactarius strains and artificial cultivation method for pine lactarius

A kind of mother seed culture medium and artificial cultivation technology, which is applied in the field of pine milk mushroom mother seed culture medium and pine milk mushroom artificial cultivation, can solve the problems such as the inability to industrialize the production of pine milk mushroom, and achieve the effect of consistent morphological characteristics

Inactive Publication Date: 2002-10-02
谭著明
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, people have not found a suitable culture medium for the mother species of pine milk mushroom, and a good artificial cultivation method, so it is impossible to industrialize the production of pine milk mushroom

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1--10

[0013] Table 1 lists the conditions of mycorrhizal synthesis under different medium ratios and different substrate ratios. Experimental results show that good mycorrhizal synthetic shoots can be obtained by using the present invention to provide the mother seed medium, and under the culture conditions of the present invention.

[0014] special

sign

sky

number

matrix

Granular Carbon 70% + Vermiculite 20%

Granular carbon 40% + vermiculite 50%

Culture medium number

1 2 3 4 5

1 2 3 4 5

Seedling

situation

7

14

28

35

44

52

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

+2 +2 +2 +2 +2

Bacteria

situation

7

14

28

35

...

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PUM

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Abstract

A culture medium for mother strains of pine lactarius is prepared from yeast (2-10g), nicotinic acid (1-10 mg), folic acid (2-8mg) and biotin (1-8 mg). An artificial method for cultivating said pine lactarius with said culture medium includes cultivating mycorrhizic seedlings with the seeds of masson pine and culture and planting the mycorrhizic seedlings in conventional condition for 2-3 years to grow said mushroom. Its advantage is the realization of industrial production.

Description

field of invention [0001] The invention relates to a culture medium for mother species of pine milk mushroom and an artificial cultivation method of pine milk mushroom. Background technique [0002] Pine milk mushroom (Lactarius deliciosus) is a very popular edible fungus, but there is no successful artificial cultivation method at present. Seen reports have Fang Pingyi's attempt to artificially cultivate Pine Milk Mushroom ("Edible Fungus" 1994.3). The method adopted is: 1. Adopt MS basic medium, remove auxin and cytokinin, add 3g / l peptone, 2g / l yeast extract, agar 2% as split hyphae, mother species culture and preservation culture 2. The original seed medium is a mixture of wheat or cow dung powder and rice; 3. The fruiting body formation medium is cottonseed shell, rotten thatch and sucrose; Part of the caps at the place were cultured on the slant medium. After the hyphae appeared, the hyphae were selected to transfer and cultured into the mother species, and then the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C12N1/14
Inventor 谭著明
Owner 谭著明
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