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Retinol binding protein detecting kit

A detection kit and protein-binding technology, applied in biological testing, measurement devices, material testing products, etc., can solve the problems of reducing antibody stability and detection accuracy, inaccurate reagent detection results, and poor repeatability, etc. Improve the effect and detection accuracy, increase the stability and storage time, improve the effect of accurate effect

Inactive Publication Date: 2019-01-15
浙江强盛生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Retinol-binding protein (Retinol-Binding Protein, RBP) is a transporter of vitamins in the blood, synthesized by the liver, and widely distributed in blood, cerebrospinal fluid, urine and other body fluids; the determination of RBP can detect renal tubules early It can sensitively reflect the degree of renal proximal tubule damage, and can also be used as an indicator of early renal damage and monitoring treatment; while the retinol-binding protein detection kit is used for the quantitative determination of retinol-binding protein in serum in vitro However, the antibody is prone to flocculent or flaky precipitation during storage, resulting in poor antibody performance, poor repeatability, and large coefficient of variation (CV), directly causing inaccurate test results and reducing the The stability and detection accuracy of the antibody are compromised, and it cannot effectively meet the precise detection requirements of users.

Method used

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Embodiment Construction

[0012] In order to make the technical means, creative features, goals and effects achieved by the present invention easy to understand, the present invention will be further elaborated below in conjunction with specific embodiments, but the following embodiments are only preferred embodiments of the present invention, not all. Based on the examples in the implementation manners, other examples obtained by those skilled in the art without making creative efforts all belong to the protection scope of this patent.

[0013] A retinol-binding protein detection kit includes reagent R1 and reagent R2, and the R1 reagent includes phosphate buffer saline, xanthine oxidase, polyethylene glycol-6000, paraben, calcium acetate, poly Ethylene glycol p-isooctyl phenyl ether, sodium chloride, the reagent R2 includes retinol binding protein antibody latex particles, potassium dihydrogen phosphate, inosine nucleotide, bovine serum albumin, ethylenediaminetetra Sodium acetate, mannitol, sodium a...

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PUM

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Abstract

The invention discloses a retinol binding protein detecting kit, comprising a reagent R1 and a reagent R2, wherein the R1 reagent comprises a phosphate buffer solution, xanthine oxidase, polyethyleneglycol-6000, paraben, calcium acetate, Polyethylene glycol p-isooctyl phenyl ether, and sodium chloride; and the reagent R2 comprises retinol-binding protein antibody latex particles, potassium dihydrogen phosphate, hypoxanthine nucleotide, bovine serum albumin, sodium edetate, mannitol, and sodium azide. The retinol binding protein detection kit can effectively inhibit the non-specific reaction of autoantibodies, and has a function of promoting coagulation, which effectively improves the detection effect and detection accuracy, has the characteristics of good reaction effect and high stability, and improves the user's use effect.

Description

technical field [0001] The invention relates to a retinol-binding protein detection kit. Background technique [0002] Retinol-binding protein (Retinol-Binding Protein, RBP) is a transporter of vitamins in the blood, synthesized by the liver, and widely distributed in blood, cerebrospinal fluid, urine and other body fluids; the determination of RBP can detect renal tubules early It can sensitively reflect the degree of renal proximal tubule damage, and can also be used as an indicator of early renal damage and monitoring treatment; while the retinol-binding protein detection kit is used for the quantitative determination of retinol-binding protein in serum in vitro However, the antibody is prone to flocculent or flaky precipitation during storage, resulting in poor antibody performance, poor repeatability, and large coefficient of variation (CV), directly causing inaccurate test results and reducing the The stability and detection accuracy of the antibody are compromised, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/68
CPCG01N33/54306G01N33/54393G01N33/6803G01N2333/47
Inventor 吕崇翔周永昊曹春梅
Owner 浙江强盛生物科技有限公司
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