Novel method for conveniently and sensitively detecting phosphorus contents of leymus chinensis leaves
A technology of phosphorus content and determination method, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of high sensitivity, low accuracy, high price, etc., and achieve the effect of high sensitivity and promotion of sustainable development
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[0019] The present invention will be described below through specific examples, but the present invention is not limited thereto.
[0020] The experimental methods used in the following examples are conventional methods unless otherwise specified; the reagents and biological materials used in the following examples can be obtained from commercial sources unless otherwise specified.
[0021] 1. Materials
[0022] plant material:
[0023] Leymus chinensis (Trin.)] seeds were provided by the Grassland Research Institute of the Chinese Academy of Agricultural Sciences, and the seeds were collected from Xilinhot, Inner Mongolia.
[0024] 2. Method
[0025] 1 Material cultivation:
[0026] 1) Normal Hoagland medium: Ca(NO 3 ) 2 4H 2 O 236g / L, KNO 3 102g / L, MgSO 4 ·7H 2 O98g / L, kH 2 PO 4 27g / L, K 2 SO 4 88g / L, CaCl 2 111g / L, NaH 2 PO 4 24g / L, NaNO 3 170g / L, Na 2 SO 4 21g / L, Fe-EDTA:(Na 2 EDTA 7.45g / L; FeSO 4 ·7H 2 O 5.57g / L), trace elements: H 3 BO 3 2.86g / L, ...
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