76 results about "Atpase activity" patented technology

A molecular nanomotor useful for translocating polynucleotides. The nanomotor is a multimolecular complex fueled by ATP hydrolysis. One of the motor components is an ATP-binding RNA molecule that participates in ATPase activity.

Ulcer is a serious oxidative stress induced disease with complex pathologic events including upregulation of H⁺K⁺ ATPase of parietal cell (PC) membrane, damage of mucin layer around PC, PC-DNA damage etc. The polysaccharide (GRPP) fraction and antioxidant extract (GRAOX) of ginger was examined for their ability to inhibit H⁺ K⁺ ATPase. Results indicated that the inhibition of H⁺ K⁺ ATPase activity which causes acidity in the lumen of the stomach, also exhibited better H⁺K⁺ ATPase inhibition (PPI) at the IC50 of 27.2 μg (GRPP) and 16.5 μg GAE (AOX) respectively than the known antiulcer proton pump blocker Lansoprazole (19.3 μg). Further the antioxidant activity in antioxidant extract (GRAOX) by various assay systems was examined such as Reducing power, Free radical scavenging (FRS), DNA and cytoprotection systems etc. GRAOX exhibited concentration dependent reducing power ability at 5-25 g equivalent of phenol. FRS activity with IC₅₀ of 6.8 μg equivalent of phenol etc. At 0.3 μg level GRAOX offered >80% protection to DNA and against FeSO₄-Asc'orbate induced oxidation. The major active phenolic components were identified as Gallic acid/Tannic acid (46%), and Cinnamic acid (51%).

The present invention provides a convenient assay to identify compounds with B-Raf inhibitory activity, referred to as the BRAMA (B-Raf Accelerated MEK ATPase) assay. The BRAMA assay is based on the discovery of ATPase activity of MEK, and utilizes changes in NADH concentration over time as an indicator of the production of ADP by activated MEK ATPase, where the MEK ATPase activity is activated by B-Raf. NADH concentration may conveniently be measured by Optical Density.

The invention belongs to gene engineering field. The invention establish a production method of human skeletal muscle troponin I for quick contract: recombing DNA to construct expression plasmid, refining expression products with inclusion bodies, cation exchange resin column chromatography, cation exchange resin column chromatography, renaturing inclusion bodies, at last purifying and obtaining recombinant human troponin I for suppressing blood vessel endothelial cell propagation and angiogenesis. The invention also establishes a method for determing the biological activity index of troponin I by suppressing Acto-S1 Mg-ATPase activity. The troponin I can prepare drugs of the tumor and angiogenesis related disease

A method for treating, preventing or ameliorating breast cancer is provided by administering a synergistic amount of digitoxin and either actein or an extract of black cohosh comprising triterpene glycosides, and optionally another chemopreventive agent which may be paclitaxel. Methods for treating or preventing a neoplasia using a synergistic combination, and compositions of a synergistic combination of a cardiac glycoside and either actein or an extract of black cohosh comprising triterpene glycosides, and optionally another chemopreventive agent which may be a taxane are also provided. The compositions may also be used in a method for modulating Na⁺K⁺ATPase activity. In addition, a method for inhibiting the progression or development of breast cancer in vivo by administering either actein or an extract of black cohosh comprising triterpene glycosides and optionally at least one other chemoprotective agent is provided.

The present invention relates to methods and compositions for inhibiting cell survival and/or promoting cell death following exposure to cytotoxic agents and stress such as radiation or chemotherapy exposure through inhibition of V-H⁺-ATPase. In particular, the formation and/or acidification of acidic vesicular organelles (AVOs) may be prevented or decreased by inhibiting the activity of vacuolar proton ATPase (“V-H⁺-ATPase”). The methods and compositions of the invention are based on the observation that (i) following irradiation surviving cancer cells accumulate AVOs and that their acidification is mediated by V-H⁺-ATPase; (ii) surviving colonies of cells contain higher levels of AVOs; and (iii) inhibition of V-H⁺-ATPase decreases the clonogenic survival of cells irradiated or exposed to chemotherapeutic agents. These observations led to the conclusion that V-H⁺-ATPase activity and AVO function serve to protect cells from radiation and chemotherapy damage. In addition, agents such as bFGF, TNF-α, PMA, rapamycin and tamoxifen were shown to be inducers of acidic organelle formation. Therefore signal transduction pathways mediated by these agents provide targets for drug screening assays designed to identify inhibitors of V-H⁺-ATPase activity and AVO formation/acidification. The present invention may be used to treat cancer subjects through sensitization of neoplastic cells to the toxic effects of radiation and chemotherapy.

The invention discloses a process for simultaneously preparing an antioxidative peptide and an antifreeze peptide through enzymolysis of collagens. The method comprises the following steps: extractingcollagens by taking salmon skin as a raw material, hydrolyzing the collagens into a short peptide solution by utilizing crude enzymes of extracellaluar protease obtained by fermentation of Vibrio sp.SQS2-3, performing centrifugal ultrafiltration by a 3000 Da ultrafiltration tube 4500*g for 45 minutes so as to obtain the antifreeze peptide with effects of protecting myosin and relieving decreaseof Ca<2+>-ATPase activity in multigelation, performing Sephadex LH-20 gel filtration chromatography to separate lower ultrafiltration components so as to obtain the antioxidative peptide which has effects of scavenging DPPH free radicals and hydroxyl radicals and a certain ability of inhibiting DNA oxidative damage and shows concentration-dependent antioxidant activity in oxygen radical absorptioncapacity detection. The LC-MS technology shows that the antioxidant component contains 19 polypeptides.

Micro-organisms which over-produce ATPase, especially mycoplasmas in a sample of a cell culture, are detected by making use of the ATPase activity to convert cellular or externally added ATP to ADP. The decrease in ATP externally added or the increase or absolute level of ADP produced from cellular ATP can then be detected or measured. For example, if a reagent such as phosphenol pyruvate/pyruvate kinase is added to convert that ADP back to ATP, the resulting high level of ATP can be detected bioluminescently using the reaction: Luciferin+luciferase enzyme+ATP→hv+products.

The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable substrate to produce said biomass or product. This is conveniently done by expressing in said cell the soluble part (F₁) of the membrane bound (F₀F₁ type) H⁺-ATPase or a portion of F₁ exhibiting ATPase activity. The organism from which the F₁ ATPase or portions thereof is derived, or in which the F₁ ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular, the DNA encoding F₁ or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeast, other fungi and cell lines of higher organisms and be selected from the group consisting of the gene encoding the F₁ subunit beta or a portion thereof and various combinations of said gene or portion with the genes encoding the other F₁ subunits or portions thereof. The method can be used i.a. for optimizing the formation of biomass or a desired product by a cell by expressing different levels of uncoupled ATPase activity in the cell, incubating the cell on a suitable substrate, measuring the conversion rate of substrate into biomass or the desired product at each level of ATPase expression, and choosing a level of ATPase expression at which the conversion rate is optimized.

The invention relates to a method for the microdetermination of ATPase activity of myoglobulin, and the method is characterized in that based on the reaction method of a classic molybdenum blue method, the molar ratio of ATP (adenosine-triphosphate) to myoglobulin is controlled at 2000:1 and the molar ratio of ammonium molybdate to stannous chloride is controlled at 5.5:1. The determination method provided by the invention is simple, convenient, fast, high in response value and wide in linear range, and can be applied to the screening of inhibitors. The invention discloses an application of Ruscogenin in inhibiting the ATPase activity of the myoglobulin.

A muscle protein purifying method adjusts the concentrations of KCl, ATP and ammonium sulfate (A.S.) through the influences of different solution mediums on separation and purification and analyzes the dissociation and precipitation law of all fibrillin components and determines the separation and purification conditions of actin, myoballs and paramyosin with the ATPase activity as the protein denaturation indicating parameter. The method has the advantages that compared with a traditional protein purifying method, the novel method is low in purified protein denaturation degree and simple in purifying process, the actin, myoballs and paramyosin in smooth muscles of aquatic invertebrates are efficiently obtained through purification, and the purifying cost is saved to the maximum extent.

The present invention relates to mda-5 nucleic acids, MDA-5 proteins, the mda-5 promoter, and related molecules, and the use of each of these elements in protecting against or limiting viral infection, controlling cell proliferation, and promoting apoptosis. It is based upon the discovery that, contrary to earlier hypotheses based on the amino acid sequence of MDA-5, the protein is not a defective ATPase but rather exhibits ATPase activity. This supports the role of MDA-5 as a RNA helicase protein and consequently its use in promoting RNA degradation, for example in the context of antiviral defense, limitation of cell proliferation, or induction of apoptosis.

The invention discloses application of an AtPrx64 gene in improving aluminum tolerance of plants. According to the application, the AtPrx64 gene is recombined into a plant expression vector, wild tobacco is transformed, and transgenic AtPrx64 tobacco can be screened; the experimental result shows that under the condition of aluminum stress, the relative growth rate and the soluble protein content of roots of the transgenic tobacco are gradually reduced along with increase of aluminum concentration, and the reduction rate of the transgenic tobacco is lower than that of wild tobacco; the contents of H2O2 and MDA of the transgenic tobacco are gradually increased along with increase of the aluminum concentration, and the increase rate of the transgenic tobacco is lower than that of the wild tobacco; no matter whether aluminum stress acts or not, the activity of plasmalemmas H<+>-ATPase and the secretion of citric acid of the transgenic tobacco are both higher than those of the wild tobacco; the test on the aluminum content in the roots under aluminum stress of different concentrations shows that the aluminum content in the roots of the transgenic tobacco is remarkably lower than that of the wild tobacco; the tolerance of the transgenic AtPrx64 tobacco to aluminum stress can be remarkably improved.

Atomic coordinates for human phosphotyrosyl phosphatase activator (PTPA) and ATPγS bound by PTPA, as well as methods for using these atomic coordinates to prepare ATPase inhibitors of PTPA and ATPase inhibitors prepared using such methods are provided herein. Comprehensive biochemical analyses of the interactions of PTPA with ATP and protein phosphatase 2A are also provided. Compositions including mimetics and small molecules of the invention and, optionally, secondary agents may be used to treat disorders in which PTPA ATPase activity plays a contributing role.

Changes that occur in Na⁺K⁺ ATPase regulation and therefore in the membrane potential in cells from bipolar individuals, as compared to cells from unaffected control individuals, are utilized to provide a diagnostic assay for bipolar I and bipolar II disorders. The diagnostic assay may also or instead exploit the similarity of cells from new patients to those of people already known to have bipolar I or bipolar II disorder. A similar diagnostic assay is provided for diagnosing attention-deficit/hyperactivity disorder (ADHD) and unipolar disorder. The diagnostic assays may further involve manipulation of membrane potential by incubation of cells in K⁺-free buffer and/or incubation with one or more compounds that alter Na⁺K⁺ ATPase activity. Although a variety of cells may be used, the diagnostic assays preferably employ lymphoblasts or whole blood cells.

The present invention discloses a high-affinity antibody that identifies CD39. The antibody is capable of neutralizing the ATPase activity of CD39 on cells expressing CD39. The antibody can be used for the treatment of cancer and other diseases mediated by CD39 activity.

The invention discloses application of sodium hydrosulfide in improvement of the salt resistance of corn seedlings and belongs to the field of plant stress resistance. According to application of thesodium hydrosulfide in improvement of the salt resistance of the corn seedlings, by improving the activity of PM H+-ATPase of the corn seedlings, conveying of related ions of salt stress is improved to improve the salt resistance of corn, and growth of corn plants can be promoted. The operation is easily and conveniently conducted, a certain basis is provided for research on the salt resistance ofplants, and the sodium hydrosulfide has great economic benefits and social value. According to the application, the sodium hydrosulfide is adopted as a donor of hydrogen sulfide, double distilled water is directly used for being prepared into a mother solution, and the operation is conducted easily and quickly.