8 results about "Atpase activity" patented technology

This invention provides the application of epigallocatechin gallate (EGCG) in the preparation of Bagaza virus helicase inhibitors or anti-Bagaza virus drugs, belonging to the field of pharmaceutical technology. Utilizing the crystal structure of BAGV helicase, molecular docking analysis shows that EGCG has a dual inhibitory effect on both the RNA binding site and NTP binding site of BAGV helicase, thereby precisely inhibiting helicase activity. Furthermore, ATPase activity testing results demonstrate that EGCG has a significant inhibitory effect on BAGV helicase, thus achieving the goal of anti-BAGV. This invention provides a feasible strategy for innovative BAGV therapies.

The invention belongs to the technical field of traditional Chinese medicine extraction and discloses a traditional Chinese medicine extracting solution for inhibiting sodium-potassium ATPase activity and a preparation method and application thereof.The preparation method comprises the steps that 1, traditional Chinese medicine materials capable of inhibiting sodium-potassium ATPase activity are soaked in an organic solvent, and a soaking solution is obtained; (2) performing low-temperature ultrasonic extraction on the soaking solution to obtain a crude extracting solution; (3) carrying out centrifugal separation, microporous membrane filtration and solvent evaporation on the crude extracting solution to obtain a refined extracting solution; and (4) adding the refined extracting solution into an SW620 cell culture medium to carry out cell treatment, carrying out sodium and potassium ATPase activity detection after the treatment, and taking the refined extracting solution as a target required traditional Chinese medicine extracting solution for inhibiting the sodium and potassium ATPase activity when a detection result meets an activity threshold value. In conclusion, the traditional Chinese medicine extracting solution with an excellent colon cancer resisting effect is prepared on the basis of judgment of sodium-potassium ATP enzyme activity, the preparation steps are simple, and the obtained traditional Chinese medicine extracting solution has the advantages of being easy to absorb, small in toxic and side effects and the like.

The present disclosure generally relates to cancer and the treatment of cancer, including the treatment of Alternative Lengthening of Telomeres (ALT) cancers. The disclosure provides methods comprising inhibiting FANCM activity or expression, such as inhibiting FANCM's interaction with RMI and / or FANCM's ATPase activity, to inhibit the growth and / or proliferation of ALT tumor cells and / or to induce death of ATL tumor cells. The methods may be practiced on patients diagnosed with an ALT tumor.

This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a pharmaceutical composition for treating simple obesity of spleen deficiency and dampness retention type and its application. This pharmaceutical composition is prepared based on seven traditional Chinese medicines listed in the Food and Drug Catalogue: Codonopsis pilosula, Poria cocos, Coix lacryma-jobi, Dioscorea opposita, Nelumbo nucifera, Lilium brownii, and Ziziphus jujuba. The pharmaceutical composition provided by this invention improves the condition of swollen, pale, and white-coated tongue in rats with simple obesity of spleen deficiency and dampness retention type, and improves abnormal lipid metabolism; it can effectively regulate abnormalities in serum BUN, UA, MTL, VIP, and other indicators in rats with simple obesity of spleen deficiency and dampness retention type, improve gastrointestinal emptying, enhance gastrointestinal motility, and increase the calcium content of myocardial cell membranes. 2+ -ATPase, Na + -K + - It increases ATPase activity, improves cellular energy metabolism, and reduces the risk of cardiovascular disease caused by obesity; it inhibits the expression levels of BUN and UA, which can treat obesity-induced hyperuricemia and improve renal metabolism. It also exerts a therapeutic effect on simple obese rats with spleen deficiency and dampness retention.

The present application provides a probiotic preparation and its use. The probiotic preparation of the present application comprises active ingredients and adjuvants, wherein the active ingredients comprise oxygen scavengers, organic acid producers and place holders; the adjuvants comprise flavoring agents, oligosaccharides and other additives; wherein the oligosaccharides are not lactulose, stachyose, raffinose, oligogentiobiose and oligochitosan, and the other additives are not theaflavins, ethylenediaminetetraacetic acid disodium salt and sorbic acid. The present application effectively promotes the metabolic activity of probiotics through a specific combination of adjuvants, rather than just providing physical protection or nutritional support. Experimental data show that the adjuvant combination of the present application can significantly and continuously improve the oxidation-reduction potential and Na + K + ATPase activity of probiotics. Moreover, the probiotic preparation of the present application can effectively improve functional gastrointestinal diseases and has a bidirectional regulation function, which is suitable for different intestinal motility disorders, such as diarrhea and constipation.

The invention belongs to the technical field of plant genetic engineering, and discloses a heat shock protein gene HSP90-1 for improving cadmium stress resistance of cardamine violifolia and application of the heat shock protein gene HSP90-1. The gene is a selenium-cadmium co-regulation target which is identified for the first time from a leaf transcriptome of cardamine violifolia under cadmium stress, HSP90-1 protein coded by the gene can form a Se-S bond with nano-selenium to be competitively combined with cadmium ions so as to recover ATP enzyme activity and molecular chaperone functions of the gene, so that an anti-oxidation system and a photosynthetic protection pathway of cardamine violifolia are regulated and controlled, and the anti-oxidation effect of the cardamine violifolia is improved. The cadmium stress resistance is enhanced. Not only is basic cognition on a heavy metal detoxification mechanism deepened, but also a practical solution is provided for agricultural application such as cultivation of high-HSP90-1 expression low-cadmium varieties. The method does not need to depend on exogenous elements, the cadmium stress resistance of cardamine violifolia and the soil cadmium remediation efficiency can be stably improved, and new gene resources and technical means are provided for ecological remediation of cadmium pollution.

An inhibitor for Na+-translocating V-ATPase activity including a compound represented bywhere R1 represents a group selected from a hydroxy group, an alkoxy group, and a haloalkoxy group, each bonded to an adjacent phenyl group via oxygen, or a group selected from a dialkylamino group, a heterocyclic amine, and a carboxylic acid amide group, each bonded to an adjacent phenyl group via nitrogen, or represents bromine, iodine, or a straight-chain hydrocarbon group. R2 represents hydrogen or a haloalkoxy group. Z1 represents an aliphatic hydrocarbon group, an alicyclic hydrocarbon group, an aromatic hydrocarbon group, or a heterocyclic group, each optionally having an arbitrary substituent and having a structure containing a double bond selected frombetween the group and an adjacent phenyl group. The symbol * represents a bond to the adjacent phenyl group.

The invention belongs to the field of biological medicine, and particularly relates to a novel medical application of a dopamine receptor stimulant pramipexole (PPX) as an inhibitor of the activity of sapovirus 2C RNA helicase ATPase, and the inhibitor is pramipexole. In-vitro enzymology experiments prove that the pramipexole can remarkably inhibit the ATP enzyme activity of the SaV 2C RNA helicase, and the inhibition effect of the pramipexole is quantitatively verified through a malachite green-ammonium molybdate colorimetric method. The invention further discloses that pramipexole and SaV-2C RNA helicase form stable interaction (the binding energy is-126.53 kJ / mol) through molecular docking, and the key interaction comprises four hydrogen bonds mediated by Asp47 and Glu48 residues, hydrophobic interaction between the four hydrogen bonds and Leu30, Ala31 and Trp46, and electrostatic force generated by the four hydrogen bonds and Glu48. The invention has the potential of preventing and treating sapovirus infection diseases.