Water environment health risk assessment method based on zebrafish protein biological responses

Abstract

The invention discloses a water environment health risk assessment method based on zebrafish protein biological responses. According to the method, a zebrafish exposure test is carried out, various protein biomarkers in zebrafish are quantitatively analyzed at the same time through utilization of a high performance liquid chromatography-mass spectrometry, and uniform quantization is carried out onbiomarker responses according to zebrafish protein response indexes, so a health risk level and degree of exposed water are determined. According to the method, differential expressions of various protein biomarkers in the zebrafish under water environment medium exposure are measured and the biomarkers are quantized and integrated into the zebrafish protein response indexes, so a health risk ofa water environment is represented quantitatively.

Description

technical field [0001] The invention belongs to the technical field of environmental monitoring and risk assessment, and relates to a water environment health risk assessment method using protein biological effects as indicators, in particular to a method for comprehensively quantifying multiple protein organisms in zebrafish (Danio rerio) exposed to water environment media. Differential expression of markers, and a method for quantitatively assessing water environment health risks. Background technique [0002] The water environment is an important part of human survival and development. However, since 1993, about 53% of the more than 1,000 serious and serious environmental emergencies that have occurred in my country are water pollution accidents. Compared with the increasingly serious water environment pollution risk, my country's water environment risk assessment ability is relatively weak. Health risk assessment originated in the 1980s. It uses risk as an evaluation in...

AI Technical Summary

Problems solved by technology

However, in practical applications, this type of health risk assessment method based on pollutant concentration indicators has certain shortcomings: first, it is often impossible to identify all pollutants in the water environment in practical applications; second, not all pollutants have Corresponding toxicity test data; 3. Unable to evaluate compound effects

Due to these limitations, such water environment health risk assessment models often cannot comprehensively reflect the real water environment health risks under actual conditions.

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