A kind of α-glucan with oxygen pressure exclusion effect and its preparation method and application

A glucan, oxygen pressure technology, applied in microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problem of not finding alpha-glucan, and achieve good dissolved oxygen removal. effect, the effect of relieving oxygen stress, reducing the level of dissolved oxygen

Active Publication Date: 2022-04-01
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, no α-glucan with oxygen stress exclusion effect has been found

Method used

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  • A kind of α-glucan with oxygen pressure exclusion effect and its preparation method and application
  • A kind of α-glucan with oxygen pressure exclusion effect and its preparation method and application
  • A kind of α-glucan with oxygen pressure exclusion effect and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method of α-glucan with oxygen pressure exclusion effect, the method comprises the following steps:

[0027] (1) Leuconostoc enterococci CGMCC10064 was inoculated into TYC medium at an inoculum size of 3% (v / v), vibrated and fermented at 30°C for 48 hours at a speed of 180 rpm to obtain a fermentation broth;

[0028] Wherein, the preparation method of Leuconostoc mesenteroides CGMCC10064 seed solution is as follows: dissolve the freeze-dried powder of Leuconostoc mesenteroides CGMCC10064 with a small amount of sterile distilled water, and take a ring with an inoculation loop to mark a line in a 5% sucrose-containing MRS solid medium (purchased from OXOID, UK), 30 ° C aerobic culture for 48h, taken out with an inoculation loop to pick a single colony into 10mL MRS liquid medium containing 5% sucrose (purchased from OXOID, UK), using a vortex Rotate the shaker to evenly disperse the colony in the liquid medium, 30 ° C, 180rpm shaking culture for 24 hours, ta...

Embodiment 2

[0033] A preparation method of α-glucan with oxygen pressure exclusion effect, the method comprises the following steps:

[0034] (1) Inoculate Leuconostoc enterococcus CGMCC10064 into TYC medium at an inoculum size of 2% (v / v), vibrate and ferment for 72 hours at 28° C., and vibrate at a speed of 200 rpm to obtain a fermentation broth;

[0035]Wherein, the preparation method of Leuconostoc mesenteroides CGMCC10064 seed solution is as follows: dissolve the lyophilized powder of Leuconostoc mesenteroides CGMCC10064 with a small amount of sterile distilled water, and take a ring with an inoculation loop to draw a line on a seed containing 4% sucrose. MRS solid medium (purchased from OXOID, UK), 30 ° C aerobic culture for 48h, taken out with an inoculation loop to pick a single colony into 10mL MRS liquid medium containing 4% sucrose (purchased from OXOID, UK), using a vortex Rotate the shaker to evenly disperse the colony in the liquid medium, take it out after 24 hours of shaki...

Embodiment 3

[0039] A preparation method of α-glucan with oxygen pressure exclusion effect, the method comprises the following steps:

[0040] (1) Inoculate Leuconostoc enterococcus CGMCC10064 into TYC medium at an inoculum size of 2% (v / v), vibrate and ferment for 72 hours at 32° C., and vibrate at a speed of 150 rpm to obtain a fermentation broth;

[0041] Wherein, the preparation method of Leuconostoc mesenteroides CGMCC10064 seed solution is as follows: dissolve the freeze-dried powder of Leuconostoc mesenteroides CGMCC10064 with a small amount of sterile distilled water, and take a ring with an inoculation loop to draw a line on a 6% sucrose-containing MRS solid medium (purchased from OXOID, UK), 32 ° C aerobic culture for 48h, taken out with an inoculation loop to pick a single colony into 10mL MRS liquid medium containing 6% sucrose (purchased from OXOID, UK), using a vortex Rotate the shaker to evenly disperse the colony in the liquid medium, take it out after 24 hours of shaking c...

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Abstract

The present invention provides an α-glucan with an oxygen pressure exclusion effect and its preparation method and application. The α-glucan has a main chain connected by α-(1,6) glycosidic bonds, and α ‑(1,4), α‑(1,3) glycosidic bonds connected branched chains, glycosidic bonds α‑(1,6), α‑(1,4), α‑(1,3) The molar ratio is 1:0.45~0.5:0.4~0.45, and its relative molecular mass is 1.0x10 5 ~5.0x10 6 Da. The α-glucan is produced by fermentation of Leuconostoc enterococcus CGMCC10064. The α-glucan of the present invention exhibits a good effect of removing dissolved oxygen in aqueous solution, and can significantly reduce the level of dissolved oxygen in a liquid environment, thereby alleviating the oxygen pressure of probiotics. The glucan is derived from Leuconostoc enterococci subspecies, which is recognized as safe, and is expected to be widely used in probiotics and related food industries.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an alpha-glucan with an oxygen pressure exclusion effect and a preparation method and application thereof. Background technique [0002] Dextran refers to a homopolysaccharide composed of glucose as a monosaccharide, and the glucose units are connected by glycosidic bonds. Dextran has a relatively high molecular weight, mainly connected by D-glucopyranose with α-(1,6) bonds, and the branch points are α-(1,2), α-(1,3), α- (1,4) connected. According to the nature of dissolved water, it can be divided into soluble and insoluble. According to the type of glycosidic bond, it can be divided into α-glucan and β-glucan. α-glucan is a polysaccharide produced by the fermentation of sucrose by Leuconostoc enterica. The main chain is composed of α-D glucopyranose via α-(1,6) glycosidic bonds. With the change of microbial species and culture conditions, the structure size and comp...

Claims

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Application Information

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Patent Type & AuthorityPatents(China)
IPC IPC(8): C12P19/08C12N1/20C12N1/38C12R1/01C12R1/25
CPCC12N1/20C12N1/38C12P19/08
Inventor鄢明辉刘振民游春苹扶晓菲
OwnerBRIGHT DAIRY & FOOD CO LTD