Alpha-glucan with oxygen pressure elimination effect, preparation method and application thereof
A technology of dextran and oxygen pressure, applied in the direction of microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of not finding α-glucan and achieve good dissolved oxygen exclusion effect, reducing dissolved oxygen levels, and relieving oxygen stress
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Embodiment 1
[0026] A preparation method of α-glucan with oxygen pressure exclusion effect, the method comprises the following steps:
[0027] (1) Leuconostoc enterococci CGMCC10064 was inoculated into TYC medium at an inoculum size of 3% (v / v), vibrated and fermented at 30°C for 48 hours at a speed of 180 rpm to obtain a fermentation broth;
[0028] Wherein, the preparation method of Leuconostoc mesenteroides CGMCC10064 seed solution is as follows: dissolve the freeze-dried powder of Leuconostoc mesenteroides CGMCC10064 with a small amount of sterile distilled water, and take a ring with an inoculation loop to mark a line in a 5% sucrose-containing MRS solid medium (purchased from OXOID, UK), 30 ° C aerobic culture for 48h, taken out with an inoculation loop to pick a single colony into 10mL MRS liquid medium containing 5% sucrose (purchased from OXOID, UK), using a vortex Rotate the shaker to evenly disperse the colony in the liquid medium, 30 ° C, 180rpm shaking culture for 24 hours, ta...
Embodiment 2
[0033] A preparation method of α-glucan with oxygen pressure exclusion effect, the method comprises the following steps:
[0034] (1) Inoculate Leuconostoc enterococcus CGMCC10064 into TYC medium at an inoculum size of 2% (v / v), vibrate and ferment for 72 hours at 28° C., and vibrate at a speed of 200 rpm to obtain a fermentation broth;
[0035]Wherein, the preparation method of Leuconostoc mesenteroides CGMCC10064 seed solution is as follows: dissolve the lyophilized powder of Leuconostoc mesenteroides CGMCC10064 with a small amount of sterile distilled water, and take a ring with an inoculation loop to draw a line on a seed containing 4% sucrose. MRS solid medium (purchased from OXOID, UK), 30 ° C aerobic culture for 48h, taken out with an inoculation loop to pick a single colony into 10mL MRS liquid medium containing 4% sucrose (purchased from OXOID, UK), using a vortex Rotate the shaker to evenly disperse the colony in the liquid medium, take it out after 24 hours of shaki...
Embodiment 3
[0039] A preparation method of α-glucan with oxygen pressure exclusion effect, the method comprises the following steps:
[0040] (1) Inoculate Leuconostoc enterococcus CGMCC10064 into TYC medium at an inoculum size of 2% (v / v), vibrate and ferment for 72 hours at 32° C., and vibrate at a speed of 150 rpm to obtain a fermentation broth;
[0041] Wherein, the preparation method of Leuconostoc mesenteroides CGMCC10064 seed solution is as follows: dissolve the freeze-dried powder of Leuconostoc mesenteroides CGMCC10064 with a small amount of sterile distilled water, and take a ring with an inoculation loop to draw a line on a 6% sucrose-containing MRS solid medium (purchased from OXOID, UK), 32 ° C aerobic culture for 48h, taken out with an inoculation loop to pick a single colony into 10mL MRS liquid medium containing 6% sucrose (purchased from OXOID, UK), using a vortex Rotate the shaker to evenly disperse the colony in the liquid medium, take it out after 24 hours of shaking c...
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