Fructus lycii arabinogalactan as well as preparation method and application thereof

A technology of arabinose and galactose, applied in the fields of application, food science, nervous system diseases, etc.

Active Publication Date: 2019-02-22
SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has the effects of lowering blood fat, lowering blood sugar, protecting the liver, anti-tumor, and immune regulation, but there is no report on the effect of Lycium barbarum polysaccharide on the treatment of Alzheimer's disease

Method used

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  • Fructus lycii arabinogalactan as well as preparation method and application thereof
  • Fructus lycii arabinogalactan as well as preparation method and application thereof
  • Fructus lycii arabinogalactan as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of Lycium barbarum polysaccharide LBP1A1-1

[0035] a. Polysaccharide extraction:

[0036] 1000g of dried Lycium barbarum fruit was pulverized by a pulverizer, and 20L of deionized water was added. At 55°C, 30g of cellulase, 10g of amylase, and 5g of papain were added to extract for 1 hour, and then the temperature was raised to 100°C to inactivate the enzyme. live, centrifuged, the filtrate was concentrated, and dialyzed against flowing water for 3 days. Heat and concentrate the dialyzed internal solution to 3 L, centrifuge to discard the precipitate, add five times the volume of 15 L of 95% ethanol to the supernatant under stirring, let it stand overnight, and separate by centrifugation, and the obtained precipitate is alternately washed with absolute ethanol and acetone for 3 The second time, centrifuged, and the precipitate was vacuum-dried at 50°C to obtain 25.8 g of crude polysaccharide from Lycium barbarum with enzyme-hydration extraction....

Embodiment 2

[0055] Example 2. Arabinogalactan LBP1A1-1 inhibits Aβ 42 generation

[0056]1. CHO / APPBACE1 and HEK 293-APP sw Aβ in cells 42 ELISA detection

[0057] CHO / APPBACE1 cells (from Shanghai Institute of Materia Medica, Chinese Academy of Sciences) were cultured in Ham's F12 medium containing 10% fetal bovine serum (purchased from Gibco, USA), 100 U / ml penicillin and 100 μg / ml streptomycin American Hyclone Company). HEK293-APP sw (Donated by Zhang Haiyan's research group in our institute) Cells were cultured in DMEM high-glucose medium containing 10% fetal bovine serum, 100U / ml penicillin, 100μg / ml streptomycin and 200μg / ml, until the cells grew to 80% -90% confluence at 5×10 5 The density of cells / well was seeded in a 24-well plate in 5% CO 2 , after culturing in an incubator at 37°C for 24 hours, adding Lycium barbarum polysaccharide LBP1A1-1 (Example 1), after 24 h, the cell supernatant was collected.

[0058] Human Aβ 42 ELISA kit (purchased from Invitrogen, USA) to d...

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Abstract

The invention relates to a polysaccharide LBP1a1-1 extracted from fructus lycii, application of the polysaccharide in preparation of medicines or health care products for preventing and/or treating neurodegenerative diseases, and application of the polysaccharide in preparation of medicines or health care products for inhibiting Abeta42 and in preparation of medicines or health care products for preventing and treating Alzheimer's disease. Specifically, the invention relates to arabinogalactan extracted from the fructus lycii. The preparation method comprises the following steps: extracting crude polysaccharides in the fructus lycii by combining cellulase, amylase and papain with water of 55 DEG C, and performing alcohol precipitation; and identifying by combining multiple column chromatography purification methods with spectrum analysis, thereby obtaining the arabinogalactan. In vitro experiments prove that the polysaccharide is capable of inhibiting production of the Abeta42 in CHO/APPBACE1 cells for stable transfection of APP and BACE1 in a dosage dependent manner. Therefore, the polysaccharide has a potential effect of treating the Alzheimer's disease, and is expected to be developed into a carbohydrate drug for treating the Alzheimer's disease.

Description

technical field [0001] The present invention relates to polysaccharides, their extraction methods and their use in the preparation of medicines, more specifically, to a method of extracting arabinogalactan polysaccharide LBP1A1-1 from red wolfberry (fruits of Lycium barbarum L.) The method, the arabinogalactan polysaccharide extracted by the method and the use of the arabinogalactan polysaccharide in the preparation of drugs or health products for the prevention and / or treatment of neurodegenerative diseases, in the preparation of drugs for inhibiting Aβ 42 The use of the generated medicine or health care product and the use in the preparation of medicine or health care product for treating Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD), also known as Alzheimer's disease, is a progressive neurodegenerative disease. The main clinical manifestations of AD are memory loss, cognitive dysfunction, behavioral abnormalities and social barriers. Wait. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P25/28
CPCA23V2002/00A23L33/125C08B37/0003C08B37/006A23V2200/322A23V2250/503A23V2300/14
Inventor 丁侃周立爽廖文锋
Owner SHANGHAI INST OF MATERIA MEDICA CHINESE ACAD OF SCI
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