Detecting method of lead-cadmium mixed-exposure marker related to sex-hormone abnormality and application thereof
A detection method and a technology of sex hormones, which are applied in the fields of analytical chemistry and clinical medicine, can solve problems that have not received corresponding attention, and achieve the effects of delaying and preventing disease progression, preventing sample contamination, and rapid and accurate instrument analysis
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0081] Embodiment 1 Research Object Selection and Grouping Basis
[0082] The inventors collected whole blood samples from cases of abnormal sex hormones and normal controls meeting the requirements from Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University. Those with normal sex hormone examination were regarded as controls, and those with abnormal sex hormone examination were regarded as cases. 100 cases were randomly included, and 100 people were matched according to age and BMI. A total of 200 people were used as experimental subjects for the evaluation of mixed exposure to lead and cadmium with abnormal sex hormones. The specific sample classification criteria are as follows:
[0083] The first stage of association exploration
[0084] 200 cases were included, and 200 matched controls were matched according to age and BMI, a total of 400 people.
[0085] Group A: healthy control group (200 people, normal sex hormone levels):
[0086] 1. B...
Embodiment 2I
[0095] Example 2 Analysis of ICP-MS detection of mixed exposure to lead and cadmium and risk of abnormal sex hormones
[0096] 1. Sample pretreatment
[0097] Take 100 μl of whole blood, add 4.9ml of reagent A (that is, dilute 50 times), and mix well.
[0098] 2. Instrument testing
[0099] Analytical instrument: ICAP RQ ICP-MS (Thermo)
[0100]a. After ICP-MS is ignited and started, it is stable for about half an hour; pump 1 ppb tuning solution (provided by the instrument manufacturer, Ba, Bi, Ce, Co, Ln, Li, U, each concentration is 1 μg / L, and the matrix is 2.5 %HNO 3 , 0.5% HCl), according to the sensitivity of Li, In and U, the oxide (CeO) and the double charge (Ba 2+ ) level, tune the entire mass range (4 ~ 290amu) and achieve the best state; tuning requirements meet the following standards: standard mode sensitivity 7Li≥4000cps / ppb, 115In≥15000cps / ppb; 238U≥20000cps / ppb; standard mode Double charged Ba 2+ / Ba<3.0%; standard mode oxide 156CeO / 140Ce<2.0%; optimiz...
Embodiment 3
[0114] Example 3 Production of a kit for detecting mixed exposure to lead and cadmium in whole blood to aid in the diagnosis of abnormal sex hormones
[0115] Firstly, the ICP-MS method was used to determine the high levels of lead and cadmium concentrations in the whole blood of the case group, and then the chi-square test was used to find that the composition ratios of individuals with different exposure levels in the healthy control group and the case group were significantly different. The number of people with high exposure was more than that of the control group, and high exposure to lead and cadmium significantly increased the incidence of abnormal sex hormones. Detection of mixed exposure to lead and cadmium can be used for auxiliary diagnosis of abnormal sex hormones. This kit includes a batch of reagents and consumables for the detection of lead and cadmium in whole blood, in which the diluent is used for sample dilution, which contains a stable and detectable intern...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com