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Detecting method of lead-cadmium mixed-exposure marker related to sex-hormone abnormality and application thereof

A detection method and a technology of sex hormones, which are applied in the fields of analytical chemistry and clinical medicine, can solve problems that have not received corresponding attention, and achieve the effects of delaying and preventing disease progression, preventing sample contamination, and rapid and accurate instrument analysis

Inactive Publication Date: 2019-02-22
NANJING MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of ICP-MS in the auxiliary diagnosis of sex hormone abnormalities has not received corresponding attention

Method used

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  • Detecting method of lead-cadmium mixed-exposure marker related to sex-hormone abnormality and application thereof
  • Detecting method of lead-cadmium mixed-exposure marker related to sex-hormone abnormality and application thereof
  • Detecting method of lead-cadmium mixed-exposure marker related to sex-hormone abnormality and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Embodiment 1 Research Object Selection and Grouping Basis

[0082] The inventors collected whole blood samples from cases of abnormal sex hormones and normal controls meeting the requirements from Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University. Those with normal sex hormone examination were regarded as controls, and those with abnormal sex hormone examination were regarded as cases. 100 cases were randomly included, and 100 people were matched according to age and BMI. A total of 200 people were used as experimental subjects for the evaluation of mixed exposure to lead and cadmium with abnormal sex hormones. The specific sample classification criteria are as follows:

[0083] The first stage of association exploration

[0084] 200 cases were included, and 200 matched controls were matched according to age and BMI, a total of 400 people.

[0085] Group A: healthy control group (200 people, normal sex hormone levels):

[0086] 1. B...

Embodiment 2I

[0095] Example 2 Analysis of ICP-MS detection of mixed exposure to lead and cadmium and risk of abnormal sex hormones

[0096] 1. Sample pretreatment

[0097] Take 100 μl of whole blood, add 4.9ml of reagent A (that is, dilute 50 times), and mix well.

[0098] 2. Instrument testing

[0099] Analytical instrument: ICAP RQ ICP-MS (Thermo)

[0100]a. After ICP-MS is ignited and started, it is stable for about half an hour; pump 1 ppb tuning solution (provided by the instrument manufacturer, Ba, Bi, Ce, Co, Ln, Li, U, each concentration is 1 μg / L, and the matrix is ​​2.5 %HNO 3 , 0.5% HCl), according to the sensitivity of Li, In and U, the oxide (CeO) and the double charge (Ba 2+ ) level, tune the entire mass range (4 ~ 290amu) and achieve the best state; tuning requirements meet the following standards: standard mode sensitivity 7Li≥4000cps / ppb, 115In≥15000cps / ppb; 238U≥20000cps / ppb; standard mode Double charged Ba 2+ / Ba<3.0%; standard mode oxide 156CeO / 140Ce<2.0%; optimiz...

Embodiment 3

[0114] Example 3 Production of a kit for detecting mixed exposure to lead and cadmium in whole blood to aid in the diagnosis of abnormal sex hormones

[0115] Firstly, the ICP-MS method was used to determine the high levels of lead and cadmium concentrations in the whole blood of the case group, and then the chi-square test was used to find that the composition ratios of individuals with different exposure levels in the healthy control group and the case group were significantly different. The number of people with high exposure was more than that of the control group, and high exposure to lead and cadmium significantly increased the incidence of abnormal sex hormones. Detection of mixed exposure to lead and cadmium can be used for auxiliary diagnosis of abnormal sex hormones. This kit includes a batch of reagents and consumables for the detection of lead and cadmium in whole blood, in which the diluent is used for sample dilution, which contains a stable and detectable intern...

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Abstract

The invention belongs to the fields of analytical chemistry and clinical medicine and discloses a detecting method of a lead-cadmium mixed-exposure marker related to sex-hormone abnormality and an application thereof. The lead-cadmium mixed-exposure marker related to sex-hormone abnormality is the lead-cadmium mixed-exposure marker. The detecting method adopts ICP-MS to detect the concentration oflead and cadmium in whole blood, can be used for auxiliary diagnosis on the sex-hormone abnormality, is less in blood use amount, is simple, fast and accurate in operation and has better clinical promotion value.

Description

field of invention [0001] The invention belongs to the field of analytical chemistry and clinical medicine, and relates to an exposure biomarker related to abnormal sex hormones and its detection method and application. The exposure biomarker is mixed exposure of lead and cadmium, and the detection method is based on ICP-MS method. Background technique [0002] Sex hormones are a general term for steroid hormones regulated by the hypothalamic-pituitary-gonadal axis. Sex hormones include androgens, estrogens, and progestins. The synthesis and metabolism of sex hormones refers to the process in which cholesterol is catalyzed by a series of enzymes to generate progesterone, androgen, and estrogen under the regulation of the HPT axis. [1] . . Causes of abnormal sex hormones include living area, disease, environmental pollution, etc. [2-4] . Accumulating evidence for the importance of gonadal hormone action for the function of the reproductive and other systems [5,6] . Am...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N27/62
Inventor 夏彦恺杨静孙娴杨旭董天宇陈敏健王心如
Owner NANJING MEDICAL UNIV
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