Capric acid and prostaglandin E2 combination as giant baby auxiliary diagnosis marker and application thereof
An auxiliary diagnosis and prostaglandin technology, applied in the fields of analytical chemistry and clinical medicine, can solve problems such as low sensitivity and complicated experimental steps, and achieve the effect of improving sensitivity and specificity, easy detection, delaying and preventing disease progression
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Embodiment 1
[0092] Embodiment 1 Research Object Selection and Grouping Basis
[0093] The present inventor collected blood samples from mothers of macrosomia patients and normal children who met the requirements from the Nanjing Maternal and Child Health Hospital Affiliated to Nanjing Medical University (see figure 1 , figure 2 ). Weigh the newborn after birth, and if the weight is equal to or greater than 4kg within 1 hour after birth, it is diagnosed as "macrosomia". In the first stage, 97 samples of pregnant women who met the requirements were randomly included, 15 of which were macrosomia; in the second stage, 30 samples of pregnant women were included, 15 of which were compared with 15 cases of macrosomia, as the blood metabolism of small molecular organisms during pregnancy of macrosomia. Screening subjects for markers. Specific sample classification criteria are as follows:
[0094] first stage screening
[0095] A total of 97 pregnant women were randomly included.
[0096] ...
Embodiment 2
[0109] Example 2: UPLC-MS Metabolomics Biomarker Screening for Macrosomia
[0110] 1. Sample pretreatment
[0111] 1.1. Centrifuge fresh pregnant blood at 3000rpm for 5min, take 100μL supernatant and put it into a clean 1.5ml EP tube.
[0112] 1.2. Add 50 μL of water to 50 μL of supernatant and add 300 μL of methanol (reagent A) to precipitate protein.
[0113] 1.3. Aspirate the supernatant, blow dry with nitrogen and then vacuum dry.
[0114] 1.4. Reconstitute with 20 μL of the dried product (reagent D).
[0115] 2. Instrument testing
[0116] 2.1. Analytical instruments: UPLC Ultimate 3000 system (Dionex) high-performance liquid chromatography; Q-Exactive high-resolution mass spectrometer.
[0117] 2.2. Liquid phase conditions:
[0118] 2.2.1. The liquid chromatographic column is a Hypersil GOLD C18 chromatographic column (100mm×2.1mm, particle size 1.9μm, Thermo Scientific, Germany), and the column temperature is 40°C.
[0119] 2.2.2 Mobile phase A is water containing...
Embodiment 3
[0131] Example 3 Stability Analysis of Blood Metabolism Small Molecules
[0132] The method of Example 2 was used to evaluate the stability of the combination level of capric acid and prostaglandin E2 in the mother's blood during pregnancy (the interval was 2 weeks). The results showed that combined capric acid and prostaglandin E2 levels in blood were stable ( Figure 4 ), with properties as diagnostic / monitoring markers.
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