In-vitro animal organ perfusion device

Abstract

The invention relates to an in-vitro animal organ perfusion device which comprises a liquid storage component, a temperature control component, an oxygenation component and a perfusion component, wherein the liquid storage component is used for storing a perfusion solution; the temperature control component is used for maintaining the temperature of the perfusion solution in transportation; the oxygenation component is used for introducing oxygen into the perfusion solution in transportation to generate an oxygenation effect; the perfusion component comprises an outer-layer heat insulation subsystem and an inner-layer perfusion subsystem, the inner-layer perfusion subsystem comprises a perfusion chamber with a cover, a perfusion tube and a bile drainage tube are arranged on the cover, theperfusion chamber is divided into an upper layer and a lower layer by a perforated partition for holding a to-be-perfused animal liver, and the lower layer is connected to the liquid storage componentthrough a recovery pipeline. The in-vitro animal organ perfusion device provided by the invention has the benefits that a controllable temperature environment and a perfusion solution oxygen concentration environment are provided on the basis of realizing an animal perfusion technique; more changeable and perfect experimental conditions can be provided for a related in-vitro animal organ tissue experiment; in the experiment process, a temperature probe, an oxygen concentration probe and a pH probe of the device can provide more experiment data.

Description

technical field [0001] The invention belongs to the technical field of animal experiment equipment, and in particular relates to a perfusion device for isolated animal organs. Background technique [0002] In the study of organ transplantation modeling and organ ex vivo observation / intervention in experimental animals, in vitro perfusion is a commonly used research method. The traditional in vitro perfusion technology requires each experimental center to independently design relevant in vitro perfusion and The preservation of related instruments increases the difficulty of experiment implementation, prolongs the experiment cycle, increases the cost of the experiment, and prolongs the learning cycle of researchers to a certain extent. [0003] For some animal organ transplantation experiments and organ observation experiments in vitro, there is currently a lack of a systematic in vitro perfusion and preservation device to provide a stable and controllable external environment...

AI Technical Summary

Problems solved by technology

[0004] In order to overcome the shortcomings of the above-mentioned prior art, the purpose of the present invention is to provide a perfusion device for isolated animal organs, which can realize the temperature-adjustable perfusion of animal livers such as rats / mouses in vitro, and provide more reliable and reliable The controlled external environment solves the problem of uncontrollable environmental conditions, thereby improving the stability of experimental results and reducing the difficulty of learning experimental techniques

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