Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor

A technology for pluripotent stem cells and pancreatic progenitor cells, which is applied in the field of purification method and expansion method of pancreatic progenitor cells derived from pluripotent stem cells, and can solve the problem of pancreatic progenitor cells and pancreatic progenitor cells that have not disclosed the use of pluripotent stem cells. Progenitor cell population culture is not disclosed, etc.

Inactive Publication Date: 2019-03-01
TAKEDA PHARMA CO LTD
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, Patent Document 1 does not disclose the use of pancreatic progenitor cells derived from pluripotent stem cells, and the cell population used for culture is not a uniform cell population, but a variety of cells derived from pancreatic tissue were cultured. population, there is no disclosure regarding the culture of a homogeneous population of pancreatic progenitor cells

Method used

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  • Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor
  • Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor
  • Purification method for pancreatic precursor cells derived from pluripotent stem cells and amplification method therefor

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preparation example Construction

[0153] (iPS cell production process (C process))

[0154] The culture method of the present invention may further include the step of preparing iPS cells.

[0155] Examples of methods for producing iPS cells include the methods described in the section of "pluripotent stem cells" above. The iPS cells prepared here can be induced to differentiate into pancreatic progenitor cells, and the pancreatic progenitor cells can be used in the culture of step A.

[0156] In addition, in the present invention, when pluripotent stem cells other than iPS cells are used, other pluripotent stem cells may be prepared instead of iPS cells in this step.

[0157] (Step of inducing differentiation into pancreatic progenitor cells (step D))

[0158] The culture method of the present invention may further include a step of inducing differentiation of pluripotent stem cells into pancreatic progenitor cells.

[0159] The pancreatic progenitor cells induced to differentiate here can be used in the c...

Embodiment

[0180] Hereinafter, examples are given in order to describe the present invention in more detail. However, the present invention is not limited by these Examples and the like.

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Abstract

Disclosed are: a culture method for pancreatic precursor cells derived from pluripotent stem cells, said culture method comprising step (A) for three-dimensionally culturing pancreatic precursor cellsderived from pluripotent stem cells in a medium that contains a factor belonging to the epidermal growth factor (EGF) family and / or a factor belonging to the fibroblast growth factor (FGF) family, and (2) a Wnt agonist; a production method for islet cells from pancreatic precursor cells derived from pluripotent stem cells, said production method comprising step (E) for inducing the differentiation of the pancreatic precursor cells cultured by the aforesaid method into islet cells; and a cryopreservation method for pancreatic precursor cells derived from pluripotent stem cells, said cryopreservation method comprising step (F) for freezing the pancreatic precursor cells cultured by the aforesaid method.

Description

technical field [0001] The present invention relates to a method for culturing pancreatic progenitor cells derived from pluripotent stem cells, a method for preparing islet cells from pancreatic progenitor cells derived from pluripotent stem cells, and pancreatic progenitor cells derived from pluripotent stem cells method of freezing. Furthermore, the present invention relates to a medium for culturing pancreatic progenitor cells derived from pluripotent stem cells. Background technique [0002] As a treatment for diabetes (especially insulin-dependent diabetes), pancreas transplantation and islet transplantation are effective, but there is a big problem in terms of the number of donated organs and the need to take immunosuppressants to suppress immune rejection. question. Therefore, in order to solve such a problem, isolation from pluripotent stem cells such as artificial pluripotent stem cells (iPS cells) and embryonic stem (ES) cells, and living organisms has been widel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00C12N5/0735C12N5/10
CPCC12N5/00C12N5/10C12N5/0678C12N2501/11C12N2501/117C12N2501/415C12N2506/45C12N2501/727C12N2501/155C12N2506/02C12N2501/113C12N2501/115A01N1/0221C12N5/0062C12N5/0606C12N5/0677C12N2501/119
Inventor 岩田博夫小长谷周平
Owner TAKEDA PHARMA CO LTD
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