Rapid propagation and efficient cultivation technology for resuscitation plants

A technology for reviving plants and plants, applied in the field of cultivation and utilization of scarce biological resources, can solve the problems of small population, unable to meet processing needs, small scale, etc., to achieve the effect of perfect system, solving difficulties in seed collection, and mature technology

Inactive Publication Date: 2019-03-05
INST OF BOTANY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Gesneriaceae recovery plants are distributed geographically in nature, and the population is small and small, which cannot meet the processing needs.

Method used

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  • Rapid propagation and efficient cultivation technology for resuscitation plants
  • Rapid propagation and efficient cultivation technology for resuscitation plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the multiplication of Capsule lettuce

[0039] 1. Preparation of explants

[0040] Get the fresh leaves of Capsulatum plant, rinse with tap water, then sterilize with 75% (volume percentage) alcohol aqueous solution for 10sec, then disinfect with disinfectant for 3min, then rinse with aseptic distilled water for 5min, then use a sterile scalpel on the 5-10 scratches were made on each blade.

[0041] Disinfectant: an aqueous solution containing 10g / 100mL NaClO and 0.01% (volume percentage) Tween-20.

[0042] 2. Induction

[0043] The explants obtained in step 1 were taken, placed in a culture dish with an induction medium and cultured for 15 days, and then transferred to a culture bottle with a new induction medium and cultivated for 3 weeks.

[0044] Induction medium: 1 / 2MS solid medium containing 1mg / L auxin and 3mg / L cytokinin.

[0045] Culture conditions: 25°C, 16 hours light / 8 hours dark, light intensity 3500lx.

[0046]It can be observed that aft...

Embodiment 2

[0064] Embodiment 2, the multiplication of Capsule lettuce

[0065] 1. Preparation of explants

[0066] With the step 1 of embodiment 1.

[0067] 2. Induction

[0068] The explants obtained in step 1 were taken, placed in a culture dish with an induction medium and cultured for 15 days, and then transferred to a culture bottle with a new induction medium and cultivated for 3 weeks.

[0069] Induction medium: 1 / 2MS solid medium containing 0.5mg / L auxin and 0.5mg / L cytokinin.

[0070] Culture conditions: 25°C, 16 hours light / 8 hours dark, light intensity 3500lx.

[0071] After 3 weeks in culture flasks containing new induction medium, a large number of shoots were formed in the wounds of leaves (9-11 shoots were redifferentiated per leaf on average)

[0072] 3. Rooting

[0073] After step 2 is completed, the young shoots are peeled off from the leaves, and the young shoots are placed in a culture bottle filled with a rooting medium and cultivated until the root length is 3-...

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PUM

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Abstract

The invention discloses a rapid propagation and efficient cultivation technology for resuscitation plants. The invention provides a method for expanding propagation of a resurrection plant, and the method sequentially comprises the following steps: (1) taking the explant of the resurrection plant, culturing the explant on an induction culture medium, and differentiating to obtain buds; (2) takingthe buds obtained in the step (1), and culturing the buds obtained in the step (1) on a rooting culture medium to obtain rooted buds. The method further comprises the following steps: after the step (2) is completed, taking the rooted buds, transplanting the rooted buds to a seedling hardening substrate, and sequentially carrying out seedling hardening and cultivation. The method disclosed by theinvention is mature in technology and complete in system, and can effectively solve the problem of difficulty in collecting the seeds of the resurrection plant of gesneriaceae, low natural germinationrate, and difficulty in obtaining of seedlings, the method has good popularization and application values, and the market prospect is wide.

Description

technical field [0001] The invention belongs to the technical field of cultivation and utilization of scarce biological resources, and in particular relates to a rapid propagation and high-efficiency cultivation technology of revived plants. Background technique [0002] With the scarcity of global water resources and the increase of extreme weather events, the impact of drought on plant growth, yield and quality is becoming more and more significant. After being subjected to drought stress or other adverse environmental factors, organisms will resist adverse stress through different ways, including the synthesis and accumulation of drought tolerance-related proteins, the production of protective substances, and the removal of harmful substances. [0003] Resurrection plants are considered to be excellent plant resources for studying stress resistance mechanisms and providing stress resistance genes because they can tolerate extreme drought conditions and quickly resume norm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/15A01G24/28
CPCA01H4/001A01H4/008
Inventor 邓馨孙润泽
Owner INST OF BOTANY CHINESE ACAD OF SCI
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