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Experimental method for inducing apoptosis of cancer cells HepG2 by tanshinone IIA

An experimental method, tanshinone technology, applied in the field of tanshinone IIA-induced apoptosis of cancer cell HepG2, can solve unclear problems

Inactive Publication Date: 2019-03-08
潘红莲
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Problems solved by technology

However, whether tanshinone IIA regulates P53 by regulating miR30 is still unclear.

Method used

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  • Experimental method for inducing apoptosis of cancer cells HepG2 by tanshinone IIA
  • Experimental method for inducing apoptosis of cancer cells HepG2 by tanshinone IIA
  • Experimental method for inducing apoptosis of cancer cells HepG2 by tanshinone IIA

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Embodiment Construction

[0024]An experimental method for Tanshinone IIA to induce apoptosis of cancer cell HepG2, the specific experimental process is as follows: (1) Preparation of experimental materials: Tanshinone IIA (> 98.0%); p53, Bcl2 and SHP2 antibodies; Tp53, PTPN11 designed by primer 5.0 , miR30a, b, c and d, mRNA sequences of Bax, Bcl2 and p21; Annexin V-FITC staining kit and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl Tetrazolium bromide (MTT) solution; cell culture medium DMEM and fetal bovine serum (FBS); (2) cell culture and treatment: human liver cancer cells are HepG2 and Hep3B, and the cells are treated with 10% fetal bovine serum and penicillin 100U / mL, streptomycin 100μg / mL DMEM culture solution was cultured at 37°C and 5% CO2. When confluent, the cells were treated with a series of doses of tanshinone IIA. After 24 hours, the culture medium and cells were collected and prepared for use. For the following biological assays; (3) RT-PCR assay: Extract total RNA from the cells accordi...

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Abstract

The invention discloses an experimental method for inducing the apoptosis of cancer cells HepG2 by tanshinone IIA. The experimental method comprises the following specific experimental processes: (1)preparing experimental materials; (2) culturing and treating cells; (3) performing RT-PCR determination; (4) performing Western Blot determination; (5) performing Annexin V-FITC dyeing; (6) screeningand analyzing bioinformation databases; (7) screening and determining transcription factors; (8) performing Hoechst 33324 dyeing; (9) performing in-vitro kinetic combination analysis; and (10) performing statistical analysis. The experimental method for inducing the apoptosis of the cancer cells HepG2 by the tanshinone IIA has the beneficial conditions that the apoptosis of human hepatoma cells HepG2 is realized through the induction of miR30b-p53-PTPN11 / SHP2 signal path.

Description

technical field [0001] The invention relates to the technical field of methods for treating liver cancer, in particular to an experimental method for inducing apoptosis of cancer cell HepG2 by tanshinone IIA. Background technique [0002] Over the past few decades, the incidence of liver cancer has continued to increase worldwide. From 2008 to 2010 alone, the number of deaths from liver cancer worldwide increased from 695,900 to 745,500, about half of which occurred in China. In recent years, studies have shown that the occurrence and development of liver cancer involves multiple biological signal transduction pathways. However, due to the complex pathogenesis of liver cancer, seeking effective drugs has always been a hot topic of concern. Therefore, focusing on the research of multi-target drugs is the key for pharmacologists to study the treatment of liver cancer. [0003] Traditional Chinese medicine is famous for its multi-pharmacological mechanism of action and effec...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/58A61P35/00G16B50/00G16B40/00G16B45/00G16B20/30G16B35/20C12N5/09C12Q1/686G01N33/68G01N15/14
CPCA61P35/00G01N15/10G01N15/14G01N33/6803A61K31/58C12N5/0693C12Q1/686G01N2015/103C12Q2521/107
Inventor 潘红莲
Owner 潘红莲