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Method for expanding tumor ablation range

A kind of technology with a wide range of tumors, which is applied in the direction of anti-tumor drugs, pharmaceutical formulas, parts of surgical instruments, etc., can solve the problems of cell failure and apoptosis, and achieve the effect of expanding the ablation area and improving the anti-tumor effect

Active Publication Date: 2021-01-08
SHANGHAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the problems existing in the prior art, the technical problem to be solved by the present invention is: there are still certain defects in the treatment of tumor tissue by simply using IRE technology. In this case, some tumor cells in the edge region of the tumor tissue form reversible nanopores, and these cells cannot undergo apoptosis.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Human breast cancer cells EFM-192A were cultured in cell culture medium (Dulbecco modified Egle medium, DMEM) containing 10% fetal bovine serum and 1% penicillin / streptomycin (PS), and the incubator environment was CO 2 The concentration is 5% and the temperature is 37°C. Cells were passed through 0.25% GIBCO before use ® trypsin-EDTA solution was harvested and passed through a cell counter (Countstar ® BioTech, Shanghai Ruiyu Biotech Co., Ltd., Shanghai, China) ensure that the cell concentration in the cell suspension is 2×10 6 cells / ml (overall survival rate >97%);

[0038] (2) Add human breast cancer cells EFM-192A to DMEM and place them in a cell culture dish with a cell volume of 2×10 7 , adding the conductive nanoparticles / drug-targeting composite material so that the concentration of the conductive nanoparticles / drug-targeting composite material in DMEM is 0.5 mg / mL;

[0039] (3) Implant two IRE needle-shaped electrodes in the cell culture dish, the needl...

Embodiment 2

[0041] (1) Human breast cancer cell MCF-7 was cultured in cell culture medium (Dulbecco modified Egle medium, DMEM) containing 10% fetal bovine serum and 1% penicillin / streptomycin (PS), and the incubator environment was CO 2 The concentration is 5% and the temperature is 37°C. Cells were passed through 0.25% GIBCO before use ® trypsin-EDTA solution was harvested and passed through a cell counter (Countstar ®BioTech, Shanghai Ruiyu Biotech Co., Ltd., Shanghai, China) ensure that the cell concentration in the cell suspension is 2×10 6 cells / ml (overall survival rate >97%).

[0042] (2) Take human breast cancer cell MCF-7 and add it to DMEM and place it in a cell culture dish, the cell volume is 3×10 7 , adding conductive nanoparticles / drug-targeting composite material, so that the concentration of conductive nanoparticles / drug-targeting composite material in DMEM is 0.8 mg / mL;

[0043] (3) Six IRE needle electrodes are implanted in the target area of ​​the tumor tissue in t...

Embodiment 3

[0045] (1) Human breast cancer cells MD-MB-231 were cultured in cell culture medium (Dulbecco modified Egle medium, DMEM) containing 10% fetal bovine serum and 1% penicillin / streptomycin (PS), and the incubator environment was CO 2 The concentration is 5% and the temperature is 37°C. Cells were passed through 0.25% GIBCO before use ® trypsin-EDTA solution was harvested and passed through a cell counter (Countstar ® BioTech, Shanghai Ruiyu Biotech Co., Ltd., Shanghai, China) ensure that the cell concentration in the cell suspension is 2×10 6 cells / ml (overall survival rate >97%).

[0046] (2) Human breast cancer cells MD-MB-231 were added to DMEM and placed in a cell culture dish with a cell volume of 5×10 5 , adding the conductive nanoparticles / drug-targeting composite material so that the concentration of the conductive nanoparticles / drug-targeting composite material in DMEM is 1 mg / mL;

[0047] (3) Implant 3 IRE needle electrodes in the tumor tissue target area in the c...

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Abstract

The invention relates to the technical field of biomedical engineering, in particular to a method for expanding a tumor ablation range. There are still some defects in the treatment of tumor tissue with irreversible electroporation (IRE). When the needle electrode is punctured into the target area of tumor tissue, under the action of voltage pulses, irreversible nanopores can be formed on the surface of cell membrane in the central area of the electrode needle, which can induce cell apoptosis. However, only reversible nanopores can be formed on the surface of part of tumor cells in the tumor tissue edge area, which can not reduce cell apoptosis. Aiming at the problems, the invention provides a method for expanding the tumor ablation range, the IER technology is combined with the conductivenanoparticle drug targeting technology, which can reduce the electric field threshold value in the tissue target area, and under the dual effects of the electric pulse and the anti-tumor drug, the tumor tissue cells in the center and the edge area of the target area are effectively guaranted to be completely apoptotic, and the anti-tumor effect is greatly improved.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to a method for expanding the range of tumor ablation. Background technique [0002] At present, the more effective methods for the treatment of tumor cells are mainly surgical resection, radiotherapy and chemotherapy. The above methods have significant effects on the treatment of tumor cells, but all have shortcomings. For example, surgery is particularly effective in removing solid tumors on tissues and organs, but these solid tumors must be physically accessible and able to withstand physical damage Or regeneration, surgical treatment is difficult for organs that are not easily accessible or cannot regenerate, such as brain tumors. In addition, after opening the cavity or craniotomy by surgery, an excessively large wound surface will be generated, and it is difficult to heal and recover after the operation. Radiation therapy can cause col-side damage to the surro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/00A61K9/14A61K31/4188A61K31/555A61K31/704A61K33/243A61K38/14A61K47/30A61P35/00A61B18/00
CPCA61K9/0009A61K9/146A61K31/704A61K33/243A61K31/4188A61K38/14A61K31/555A61P35/00A61B18/00A61B2018/00125A61B2018/00613
Inventor 王克敏张兵方正章文俊
Owner SHANGHAI UNIV