Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rabbit skin extract as well as preparation method and application thereof

An extract and rabbit skin technology, applied in the fields of immunology and biomedicine, can solve the problems of extract safety and reliability, environmental safety risk, time-consuming, time-consuming and labor-intensive problems

Active Publication Date: 2019-03-26
天津运三泽生物医药科技有限公司 +1
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, it is time-consuming and labor-intensive to prepare such rabbit skin extracts using traditional techniques
For example, Chinese patent applications CN1493302A, CN103110663A, CN105163746A, CN106109498A, CN106573020A all disclose the extraction method of rabbit skin extract; However, in these extraction methods, only the time-consuming of the initial extraction step has reached 6-11 days, very time consuming
At the same time, in the traditional extraction method of rabbit skin extract, it is usually necessary to use organic solvents (such as phenol), which leads to potential risks in terms of the safety and reliability of the extract and environmental safety.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Rabbit skin extract as well as preparation method and application thereof
  • Rabbit skin extract as well as preparation method and application thereof
  • Rabbit skin extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Embodiment 1. Preparation of Rabbit Skin Extract

[0085] The pox-prone skin of rabbits (New Zealand white rabbits, body weight greater than 2.5 kg) inoculated intradermally with vaccinia virus Lister strain was collected, and frozen in a freezer at -20°C in advance for future use.

[0086] (A) Take out the frozen rabbit skin from the -20°C freezer, weigh 80g, cut it into small pieces of 2-3 cm, and place it in liquid nitrogen while cutting. After all the cuttings are completed, put the rabbit skin in a pulverizer and pulverize it into particles (75-200 μm). Place the crushed rabbit skin in a beaker, seal it with plastic wrap, and wait for it to thaw to room temperature (measure once every half an hour; under normal circumstances, after liquid nitrogen treatment, the rabbit skin can reach room temperature in 2.5-3 hours ). Subsequently, the rabbit skin was placed in a constant temperature incubator at 37±2° C. for 12 hours.

[0087] (B) Put the product obtained in (A...

Embodiment 2

[0132] Example 2. Evaluation of the effect of rabbit skin extract on promoting the proliferation of oligodendrocytes

[0133] In this example, oligodendrocytes were used to study the effect of the rabbit skin extract prepared in Example 1 on promoting proliferation.

[0134] Oligodendrocytes were extracted from the cerebral cortex of SD female rats within 48 hours after birth. After five passages, the morphology of oligodendrocytes was observed under a microscope. Subsequently, cells after passage 5 were plated in poly-lysine-coated well plates. On day 1 after plating, cultured cells were identified and characterized using an oligodendrocyte-specific antibody (anti-O4 antibody) and DAPI staining.

[0135] Experimental results such as Figures 3A-3C shown. Figure 3A The results of immunofluorescence detection of cultured cells with anti-O4 antibody are shown (fluorescent micrographs). The results showed that the cultured cells were positive for anti-O4 antibody and were o...

Embodiment 3

[0141] Example 3. Evaluation of the Effect of Rabbit Skin Extract on Promoting Proliferation of Bone Marrow Mesenchymal Stem Cells

[0142] In this example, bone marrow mesenchymal stem cells in the rat femur were used to study the effect of the rabbit skin extract prepared in Example 1 on promoting proliferation.

[0143] Bone marrow mesenchymal stem cells were extracted from femurs of wistar female rats within 48 hours after birth. After 5 passages, bone marrow mesenchymal stem cells were plated in a 96-well plate at a density of 5,000 cells per well, and 7 experimental groups and 1 control group were set up, among which:

[0144] The cell culture medium that 7 experimental groups adopt is DMEM / F12 medium, wherein adds the rabbit skin extract that the concentrated embodiment 1 prepares respectively, makes its final concentration in medium be 0.2NU / ml, 0.5NU / ml respectively. ml, 1NU / ml, 1.5NU / ml, 2NU / ml, 2.5NU / ml, 3NU / ml;

[0145] The cell culture medium used in one control...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to rabbit skin extract as well as a preparation method and application thereof. Especially, the invention provides a method for preparing the rabbit skin extract and the rabbit skin extract prepared through the method. Furthermore, the invention further provides a composition containing the rabbit skin extract and the application of the rabbit skin extract.

Description

technical field [0001] This application relates to the fields of immunology and biomedicine. In particular, the present application relates to a rabbit skin extract, as well as its preparation method and use. More specifically, the present application provides a method for preparing a rabbit skin extract, and the rabbit skin extract prepared by the method. In addition, the present application also provides a composition comprising the rabbit skin extract, and uses of the rabbit skin extract. Background technique [0002] The vaccinia virus-inoculated rabbit inflamed skin extract is an extract containing non-protein active substances isolated from inflamed skin tissue of rabbits inoculated with vaccinia virus. Such extracts are liquid in the extracted state, but can also be made solid by drying. It has been reported in the literature that such extracts can be used to treat low back pain, neck, shoulder and wrist syndrome, symptomatic neuralgia, itching associated with skin...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K35/36A61P29/00A61P25/00A61P17/00A61P17/04A61P11/02A61P37/08A61P31/22A61P19/02A61P17/02A61P19/08
CPCA61K35/36
Inventor 冯世庆孔晓红姚雪冯翊坤李波孙超
Owner 天津运三泽生物医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com