Test method for peripheral blood oxidized low density lipoprotein autoantibody

A low-density lipoprotein and autoantibody technology, which is applied to material inspection products, measuring devices, instruments, etc., can solve problems such as low oxLDL levels

Inactive Publication Date: 2019-03-29
金元哲
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Problems solved by technology

A large number of clinical randomized controlled trials have also found that the current treatment and preventive measures can only reduce complications such as myocardial infarction and stroke by less than 50%.
Circulating oxLDL levels are very low due to the presence of high concentrations of antioxidant substances in the blood, and they may not fully correspond to oxidized lipoprotein levels in atherosclerotic plaques

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  • Test method for peripheral blood oxidized low density lipoprotein autoantibody

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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with examples.

[0026] First, antigen design was carried out, and the structure of Chinese oxLDL protein was analyzed using bioinformatics database and online tools, and the pH value, hydrophilicity, flexibility, surface accessibility, and antigenicity of the protein were predicted and scored. Follow the above epitope design principles and methods to select the apoB component of oxLDL to design polypeptide antigens: a. Try to be on the surface of the protein; b. Ensure that the antigen fragment sequence does not form α-helix; c. The ratio of the N-terminal and C-terminal peptides to the middle Peptides are better; d. Avoid repeating or near-repeating sequences within the protein; e. Avoid peptides with too strong homology; f. Cross-linking can be cross-linked at both ends of N and C. The end that is not very important for the production of antibodies; g. There should not be too many prolines (Pro) i...

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Abstract

The invention discloses a test method for a peripheral blood oxidized low density lipoprotein autoantibody, and relates to a preparation method for a medical antibody. The test method comprises the following steps of: a, performing antigen design, analyzing the structure of Chinese oxLDL protein by using a bioinformatics database and an online tool; and performing antibody detection, wherein the principle of ELISA is to immobilize the antigen or the antibody, and label a known antibody with a peroxidase. The antibody detection process comprises the preparation of materials and reagents, antigen preparation, sample dilution, addition of enzyme-labeled antibodies, color development, OD value determination, antibody level calculation and quality control, and quality control. The test method for the peripheral blood oxidized low density lipoprotein autoantibody uses an optimized double antibody sandwich ELISA method to detect anti-oxLDL IgG and IgM antibodies in the peripheral blood of patients, and compares the difference of peripheral blood autoantibody levels between patients with acute coronary syndrome and healthy persons, and analyzes the correlation between oxLDL antibody levelsand the occurrence, development, and even prognosis of atherosclerotic diseases, thereby determining a marker for early diagnosis of coronary atherosclerotic diseases.

Description

technical field [0001] The invention relates to a method for testing medical antibodies, in particular to a method for testing peripheral blood oxidized low-density lipoprotein autoantibodies. Background technique [0002] Atherosclerotic cardiovascular disease is the leading cause of death and disability in western countries, known as the "number one killer" in developed countries, and its incidence is also increasing in developing countries. According to the data published in the "Annual Report on Cardiovascular Diseases in China 2010", it is estimated that there are 230 million people with cardiovascular diseases in China, and 1 in every 5 adults has cardiovascular disease; 1 person died of cardiovascular disease. A large number of clinical randomized controlled trials have also found that the current treatment and preventive measures can only reduce complications such as myocardial infarction and stroke by less than 50%. Therefore, the development of newer diagnostic a...

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Application Information

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IPC IPC(8): G01N33/68G01N33/535G01N33/543
CPCG01N33/6854G01N33/535G01N33/543G01N2800/323
Inventor徐香山张学颖金元哲
Owner金元哲