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Method for mouth and nose exposure of mouse using cigarette smoke

A technology for cigarette smoke and mice, which is applied in the field of cigarette smoke exposure to the nose and mouth of mice, can solve the problem of neglecting the research on the method of exposure to the nose and mouth of mice, and achieves the effect of simple operation.

Active Publication Date: 2019-04-02
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Most of the existing studies on the inhalation exposure of smoke rodents focus on the results of biological effect indicators, ignoring the research on the method of oral and nasal exposure in mice

Method used

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  • Method for mouth and nose exposure of mouse using cigarette smoke
  • Method for mouth and nose exposure of mouse using cigarette smoke
  • Method for mouth and nose exposure of mouse using cigarette smoke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] A method for exposing the mouth and nose of mice to cigarette smoke, comprising the steps of:

[0055] Step (1), smoking cigarette smoke:

[0056] The smoking duration of each cigarette is 2s, the puffing frequency is 30s, the puffing volume is 35.0ml / 2s, and the number of puffing ports is 4 to obtain cigarette smoke;

[0057] Step (2), flue gas dilution:

[0058] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:10, and then enter the mouth-nose exposure system;

[0059] The clean air is obtained by filtering air through a high-efficiency air filter;

[0060] Step (3), reference hole and detection hole settings:

[0061] On the mouth-nose exposure system, select a sampling hole as a reference hole, and at the same time, randomly select multiple sampling holes as detection holes;

[0062] Step (4), mice are exposed to:

[0063] After the mouse was restrained in the fixed cylinder and adapted for 15 minutes, the fixed cyli...

Embodiment 2

[0084] A method for exposing the mouth and nose of mice to cigarette smoke, comprising the steps of:

[0085] Step (1), smoking cigarette smoke:

[0086] A ten-channel rotary disc smoking machine is used for smoking, the duration of each cigarette is 5s, the suction frequency is 60s, the suction volume is 50ml / 2s, and the number of suction ports is 6 to obtain cigarette smoke;

[0087] Step (2), flue gas dilution:

[0088] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:100, and then enter the mouth-nose exposure system;

[0089] The clean air is obtained by filtering air through a high-efficiency air filter;

[0090] Step (3), reference hole and detection hole settings:

[0091] On the mouth-nose exposure system, select a sampling hole as a reference hole, and at the same time, randomly select multiple sampling holes as detection holes;

[0092] Step (4), mice are exposed to:

[0093] After the mouse was restrained in the fix...

Embodiment 3

[0118] A method for exposing the mouth and nose of mice to cigarette smoke, comprising the steps of:

[0119] Step (1), smoking cigarette smoke:

[0120] A ten-channel rotary smoking machine is used for smoking, the duration of each cigarette is 4s, the puffing frequency is 40s, the puffing capacity is 40ml / 2s, and the number of puffing ports is 5 to obtain cigarette smoke;

[0121] Step (2), flue gas dilution:

[0122] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:50, and then enter the mouth-nose exposure system;

[0123] The clean air is obtained by filtering air through a high-efficiency air filter;

[0124] Step (3), reference hole and detection hole settings:

[0125] On the mouth-nose exposure system, select a sampling hole as a reference hole, and at the same time, randomly select multiple sampling holes as detection holes;

[0126] Step (4), mice are exposed to:

[0127] After the mouse was restrained in the fixed c...

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Abstract

The invention relates to a method for mouth and nose exposure of a mouse using cigarette smoke, and belongs to the technical field of biological application. The method comprises cigarette smoke suction, cigarette smoke dilution, reference hole and detection hole setting, mouse exposure, sampling, normalization of detection hole data, calculation of time coefficient of variation, calculation of spatial coefficient of variation and determination. The method for the mouth and nose exposure of the mouse using the cigarette smoke is simple to operate, takes the concentration of the cigarette smokein the exposure period as the entry point in time and space, establishes the relevant parameters of mouth and nose exposure system using the cigarette smoke, verifies the uniformity and stability ofthe concentration of the cigarette smoke particles and the distribution characteristics of the particle size in the mouth and nose exposure system using the cigarette smoke, and ensures the scientificand accuracy of the subsequent biological effects.

Description

technical field [0001] The invention belongs to the technical field of biological applications, and in particular relates to a method for exposing the mouth and nose of mice to cigarette smoke. Background technique [0002] Cigarette smoke is a mixture of particulate matter, volatile organic compounds, and gases. Among the exposure methods using animal models to study its biological effects, the oral and nasal exposure system and the whole body exposure system are the two main methods for the study of the biological effects of smoke. Compared with the whole body exposure, the oral and nasal exposure avoids animal hair Adsorption and filtration of exposed aerosols, as well as rapid distribution of aerosols in the exposure chamber and saving samples, are the main ways of acute, subacute and subchronic inhalation exposure of rodents to cigarette smoke. [0003] Most of the existing studies on the inhalation exposure of smoke rodents focus on the results of biological effect in...

Claims

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Application Information

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IPC IPC(8): G01N15/06G01N15/02G01N1/24G01N1/34A61D7/04
CPCA61D7/04G01N1/24G01N1/34G01N15/02G01N15/06
Inventor 高茜管莹向海英曾婉俐鱼涛刘欣邓乐乐杨光宇李雪梅
Owner CHINA TOBACCO YUNNAN IND
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