Method for analyzing correlation between particulate matter number concentration of cigarette smoke and expression of TNF-alpha in lung lavage fluid of mouse
A correlation analysis, cigarette smoke technology, applied in analysis materials, measurement devices, biological tests, etc., can solve problems such as the inability to fully simulate the smoking condition, and the inability to accurately compare the correlation between the effects of different cigarette smoke, and achieve good results. Reproducibility, the effect of reducing individual differences
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Embodiment 1
[0055] A method for analyzing the correlation between the number concentration of cigarette smoke particles and the expression of TNF-α in mouse lung lavage fluid, comprising the following steps:
[0056] Step (1), smoking cigarette smoke:
[0057] The suction adopts a ten-channel rotary disc smoking machine, the duration of each cigarette is 2s, the puffing frequency is 30s, the puffing capacity is 35.0ml / 2s, and the number of puffing ports is 4 to obtain cigarette smoke;
[0058] Step (2), flue gas dilution:
[0059] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:10, and then enter the mouth-nose exposure system;
[0060] The clean air is obtained by filtering air through a high-efficiency air filter;
[0061] Step (3), mice are exposed to:
[0062] After the mice were restrained in the fixed cylinder and adapted for 15 minutes, the fixed cylinder containing the mice was installed on the mouth-nose exposure system. The positi...
Embodiment 2
[0072] A method for analyzing the correlation between the number concentration of cigarette smoke particles and the expression of TNF-α in mouse lung lavage fluid, comprising the following steps:
[0073] Step (1), smoking cigarette smoke:
[0074] Sucking adopts a ten-channel rotary disc smoking machine, the smoking duration of each cigarette is 5s, the smoking frequency is 60s, the suction volume is 50ml / 2s, and the number of suction ports is 6 to obtain cigarette smoke;
[0075] Step (2), flue gas dilution:
[0076] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:100, and then enter the mouth-nose exposure system;
[0077] The clean air is obtained by continuously filtering the air through the high-efficiency air filter for 3 times;
[0078] Step (3), mice are exposed to:
[0079] After the mice were restrained in the fixed cylinder and adapted for 30 minutes, the fixed cylinder containing the mice was installed on the mouth-...
Embodiment 3
[0102] A method for analyzing the correlation between the number concentration of cigarette smoke particles and the expression of TNF-α in mouse lung lavage fluid, comprising the following steps:
[0103] Step (1), smoking cigarette smoke:
[0104] The smoking duration of each cigarette is 3s, the puffing frequency is 45s, the puffing volume is 45ml / 2s, and the number of puffing ports is 5 to obtain cigarette smoke;
[0105] Step (2), flue gas dilution:
[0106] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:30, and then enter the mouth-nose exposure system;
[0107] The clean air is obtained by continuously filtering the air through the high-efficiency air filter for 3 times;
[0108] Step (3), mice are exposed to:
[0109] After the mice were restrained in the fixed cylinder and adapted for 20 minutes, the fixed cylinder containing the mice was installed on the mouth-nose exposure system. The positive pressure smoke flow in t...
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