Method for analyzing correlation between particulate matter number concentration of cigarette smoke and expression of TNF-alpha in lung lavage fluid of mouse

A correlation analysis, cigarette smoke technology, applied in analysis materials, measurement devices, biological tests, etc., can solve problems such as the inability to fully simulate the smoking condition, and the inability to accurately compare the correlation between the effects of different cigarette smoke, and achieve good results. Reproducibility, the effect of reducing individual differences

Pending Publication Date: 2019-03-15
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the particularity of cigarette smoke, most of the current research on the influence of cigarette smoke on inflammatory factors is to first capture the total particulate matter in the smoke, and then test the effect of cigarette smoke on the expression of inflammatory factors in vitro. It cannot fully simulate the actual smoking situation, and it is impossible to accurately compare the differences in the effects of different cigarette smoke on inflammatory factors and the correlation between the two

Method used

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  • Method for analyzing correlation between particulate matter number concentration of cigarette smoke and expression of TNF-alpha in lung lavage fluid of mouse
  • Method for analyzing correlation between particulate matter number concentration of cigarette smoke and expression of TNF-alpha in lung lavage fluid of mouse
  • Method for analyzing correlation between particulate matter number concentration of cigarette smoke and expression of TNF-alpha in lung lavage fluid of mouse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] A method for analyzing the correlation between the number concentration of cigarette smoke particles and the expression of TNF-α in mouse lung lavage fluid, comprising the following steps:

[0056] Step (1), smoking cigarette smoke:

[0057] The suction adopts a ten-channel rotary disc smoking machine, the duration of each cigarette is 2s, the puffing frequency is 30s, the puffing capacity is 35.0ml / 2s, and the number of puffing ports is 4 to obtain cigarette smoke;

[0058] Step (2), flue gas dilution:

[0059] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:10, and then enter the mouth-nose exposure system;

[0060] The clean air is obtained by filtering air through a high-efficiency air filter;

[0061] Step (3), mice are exposed to:

[0062] After the mice were restrained in the fixed cylinder and adapted for 15 minutes, the fixed cylinder containing the mice was installed on the mouth-nose exposure system. The positi...

Embodiment 2

[0072] A method for analyzing the correlation between the number concentration of cigarette smoke particles and the expression of TNF-α in mouse lung lavage fluid, comprising the following steps:

[0073] Step (1), smoking cigarette smoke:

[0074] Sucking adopts a ten-channel rotary disc smoking machine, the smoking duration of each cigarette is 5s, the smoking frequency is 60s, the suction volume is 50ml / 2s, and the number of suction ports is 6 to obtain cigarette smoke;

[0075] Step (2), flue gas dilution:

[0076] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:100, and then enter the mouth-nose exposure system;

[0077] The clean air is obtained by continuously filtering the air through the high-efficiency air filter for 3 times;

[0078] Step (3), mice are exposed to:

[0079] After the mice were restrained in the fixed cylinder and adapted for 30 minutes, the fixed cylinder containing the mice was installed on the mouth-...

Embodiment 3

[0102] A method for analyzing the correlation between the number concentration of cigarette smoke particles and the expression of TNF-α in mouse lung lavage fluid, comprising the following steps:

[0103] Step (1), smoking cigarette smoke:

[0104] The smoking duration of each cigarette is 3s, the puffing frequency is 45s, the puffing volume is 45ml / 2s, and the number of puffing ports is 5 to obtain cigarette smoke;

[0105] Step (2), flue gas dilution:

[0106] Dilute and mix the cigarette smoke obtained in step (1) with clean air at a volume ratio of 1:30, and then enter the mouth-nose exposure system;

[0107] The clean air is obtained by continuously filtering the air through the high-efficiency air filter for 3 times;

[0108] Step (3), mice are exposed to:

[0109] After the mice were restrained in the fixed cylinder and adapted for 20 minutes, the fixed cylinder containing the mice was installed on the mouth-nose exposure system. The positive pressure smoke flow in t...

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Abstract

The invention relates to a method for analyzing correlation between particulate matter number concentration of cigarette smoke and expression of TNF-alpha in lung lavage fluid of a mouse, and belongsto the technical field of biological application. The method comprises the following steps: cigarette smoke suction, smoke dilution, mouse exposure, measurement of the particulate matter number concentration, lung lavage, ELISA testing, correlation analysis and judgment. The method is simple to operate, and an actual human smoking condition is simulated to the greatest extent by using a small animal mouth and nose exposure device; short or long-time exposure can be selected, follow-up survey of the biological effect of smoke on the lung is performed, and relatively complete data tracking is provided; the smoke exposure concentration is controllable, influence of different suction amounts on the lung can be simulated, and guarantee is provided for scientificity and accuracy of the biological effect influence of the smoke on the lung.

Description

technical field [0001] The invention belongs to the technical field of biological applications, and in particular relates to a correlation analysis method between the number concentration of cigarette smoke particles and the expression of TNF-α in mouse lung lavage fluid. Background technique [0002] Cigarette smoke is a mixture of particulate matter, volatile organic compounds, and gases. A variety of components in cigarette smoke can cause oxidative stress, causing the increase of reactive oxygen species in the body, disrupting the balance of cell oxidation / antioxidation, and inducing oxidative stress and inflammatory responses, in which the cytokine TNF-α in inflammatory cells play an important role in the inhibition and activation process. However, due to the particularity of cigarette smoke, most of the current research on the influence of cigarette smoke on inflammatory factors is to first capture the total particulate matter in the smoke, and then test the effect of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N33/50G01N33/558
CPCG01N1/28G01N33/5014G01N33/558
Inventor 高茜管莹刘欣米其利向海英曾婉俐李雪梅杨光宇
Owner CHINA TOBACCO YUNNAN IND
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