pH regulation based method of cultivating microalgae by using NH4HCO3 absorption liquid

A technology of microalgae cultivation and microalgae culture medium, applied in the field of microalgae cultivation that regulates and utilizes NH4HCO3 absorption liquid, can solve problems such as ammonia loss and ammonia suppression, and achieve the effects of solving high costs, promoting growth, and promoting production

Inactive Publication Date: 2019-04-12
TIANJIN UNIV
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Problems solved by technology

At the same time, the invention also solves the problems of ammonia suppression and ammonia loss in the presence of ammonium radicals as a nitrogen source

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  • pH regulation based method of cultivating microalgae by using NH4HCO3 absorption liquid
  • pH regulation based method of cultivating microalgae by using NH4HCO3 absorption liquid
  • pH regulation based method of cultivating microalgae by using NH4HCO3 absorption liquid

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Embodiment Construction

[0038] In order to illustrate the embodiments of the present invention or the technical solutions in the prior art more clearly, the following will briefly introduce the drawings that need to be used in the description of the embodiments. In order for those skilled in the art to refer to this specification, the present invention will be further explained in detail.

[0039] Step 1: Infuse Chlorella sp., L38 with 5% CO at a rate of 0.1vvm at a light intensity of 6000lux and a temperature of 30±1°C 2 +95N 2 After about 4 to 7 days of pre-cultivation, the microalgae grow to the exponential phase, and inoculated into the medium of 9 medium conditions, and the initial inoculation amount is controlled as OD 680 = 0.2. Before inoculation, it is necessary to resuspend and centrifuge the original algae solution three times under sterile conditions to wash away the nutrients in the original medium to avoid affecting the determination in the later stage of the experiment.

[0040]Step...

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Abstract

The invention relates to the field of microalgae cultivation and carbon sequestration, and in particular to pH regulation in microalgae culture and a method of cultivating microalgae by using NH4HCO3absorption liquid. The method can reduce the nitrogen loss and increase the carbon sequestration while promoting the microalgae biomass production, overcome the problem of large amount of energy consumption and nitrogen loss in a desorption process of an CO2 absorption process by ammonia water in a traditional absorption process, and reduce the cost of microalgae culture. The embodiment of the invention provides a microalgae cultivation method for feeding the NH4HCO3 absorption liquid in batches and adjusting the pH value. The microalgae is cultured in a nitrogen-free medium, and NH4HCO3 is used as a nutrient substance; and the NH4HCO3 is fed in batches to reduce ammonia nitrogen concentration and reduce ammonia toxicity; pH regulation reduces ammonia nitrogen volatilization, and allows the medium to be in a pH range suitable for microalgae growth.

Description

technical field [0001] The invention relates to the field of microalgae cultivation, in particular to the regulation of pH in microalgae cultivation and the use of NH 4 HCO 3 Microalgae culture method for absorbing liquid. Background technique [0002] In the traditional process, ammonia water absorbs CO 2 The resulting high energy consumption has always been an important issue. In order to solve the problem of energy consumption in the desorption process of the absorption liquid, this paper intends to design a CO 2 capture system. Therefore, ammonia absorbs CO 2 The main substance produced is NH 4 HCO 3 As a nitrogen and carbon source, it is added to the microalgae culture system to promote the growth of microalgae. At the same time, the algae flour produced by microalgae can extract nutrients such as polysaccharides, oils, and proteins. [0003] However, under alkaline conditions, NH 4 HCO 3 As a nutrient to cultivate microalgae, when the concentration is high, ...

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor宋春风谢美连邱依婷齐云刘庆岭纪娜
OwnerTIANJIN UNIV