A method of harvesting cells on three-dimensional microcarriers

Abstract

The invention discloses a method for harvesting cells on three-dimensional microcarriers. It includes the following steps: 1) The three-dimensional microcarriers that adsorb and culture cells are naturally settled or centrifuged, and the supernatant is removed to obtain the three-dimensional microcarriers containing cells; Microcarriers; 3) After the three-dimensional microcarriers in step 2) are completely lysed, add a stop solution to terminate the lysis process or directly proceed to step 4) to terminate the lysis; 4) Centrifuge the system obtained in step 3), discard the supernatant, and then Harvest cells on 3D microcarriers. The present invention can more gently harvest the cells on the carrier for subsequent applications; the present invention lyses the microcarriers to dissolve the microcarriers from solid to liquid, thereby releasing the cells on the carriers into the solution, and by means of centrifugation, The solution is separated from the cells, thereby obtaining the cells.

Description

A method of harvesting cells on three-dimensional microcarriers technical field [0001] The present invention relates to a method for harvesting cells on a three-dimensional microcarrier, belonging to the technical field of cell culture. Background technique [0002] With the rapid development of interdisciplinary subjects such as biomedicine, materials science, mechanics, and engineering, more and more people are concerned about Pay attention to the difference between the microenvironment of cells cultured in vitro and the microenvironment of in vivo survival. Traditional two-dimensional cell culture (based on commercial The technology has been developed for more than 100 years, in the fields of life science basic research, medical research, etc. Has a wide range of applications. However, with the rapid development of microscopic imaging technology, researchers have gradually discovered that cells in a two-dimensional culture ring The cell morphology is far away from...

AI Technical Summary

Problems solved by technology

However, after three-dimensional cell culture, multi-layered or agglomerated cells are difficult to be digested by trypsin, and the long-term digestion of trypsin or its substitutes will cause great damage to the cells themselves. Therefore, a more gentle method is needed. Methods Harvesting cells from the three-dimensional carrier, that is, by cracking the three-dimensional carrier material without damaging the cells, the efficient harvesting of cells from the three-dimensional carrier material can be achieved, thereby meeting the needs of subsequent applications

For cell culture on 3D microcarriers, there is currently no method for more gentle harvesting of cells on this carrier for subsequent applications

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