Method for determining content of kaempferol-3-O-rutinoside in safflower

A technology of rutinoside and kaempferol, which is applied in the field of content detection of active ingredients of traditional Chinese medicines, can solve the problem that the content detection, separation and purification of kaempferol-3-O-rutinoside has not yet occurred, and no kaempferol-3-O- Determination of rutin content and other issues, to achieve the effect of good detection effect, low equipment requirements, and simple detection process

Inactive Publication Date: 2019-04-16
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] But at present, there are no methods for content detection, separation and purification of kaempferol-3-O-rutinoside in safflower, although the identification and content of safflower and saffron are recorded in the 2015 edition of "Chinese Pharmacopoeia" Determination, none of which involve the determination of kaempferol-3-O-rutinoside content

Method used

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  • Method for determining content of kaempferol-3-O-rutinoside in safflower
  • Method for determining content of kaempferol-3-O-rutinoside in safflower
  • Method for determining content of kaempferol-3-O-rutinoside in safflower

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Experimental program
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Effect test

Embodiment 1

[0035] A method for determining the content of kaempferol-3-O-rutinoside in safflower, using high performance liquid chromatography to measure the content of kaempferol-3-O-rutinoside in safflower medicinal materials, the specific steps are as follows:

[0036] A. Weigh kaempferol-3-O-rutinoside reference substance, and use 70% methanol as solvent to prepare concentrations of 0.0634mg / mL, 0.1585mg / mL, 0.317mg / mL, 0.4755 mg / mL and 0.634mg / mL Five parts of reference substance solution of mL;

[0037] B. Inject the five parts of reference substance solutions obtained through step A into the high-performance liquid chromatograph with an injection volume of 10 μL respectively to obtain the corresponding chromatograms of each reference substance solution. The conditions in the high-performance liquid chromatograph are:

[0038] Chromatographic column: C18 chromatographic column, the column length is 100mm, the inner diameter is 4.6mm, and the filter membrane pore size is 3.5um;

...

Embodiment 2

[0047] In this example, the content of kaempferol-3-O-rutinoside in the safflower medicinal material with the batch number S201810005 was determined, and the samples were injected in parallel six times, and the rest were processed in the same way as in Example 1. The results are shown in Table 1. Show:

[0048]

[0049] It can be seen from Table 1 that the relative standard deviation (RSD) of this determination method is 0.161%, and the precision of the test method meets the requirements.

Embodiment 3

[0051] In this embodiment, the content of kaempferol-3-O-rutinoside in the safflower medicinal material whose batch number is S201810005 is determined, and six parts of the test solution are processed in parallel, and the samples are injected once respectively, and the rest are the same as in Example 1. The processing method, the results are shown in Table 2:

[0052]

[0053] It can be seen from Table 2 that the relative standard deviation RSD of this determination method is 0.870%, and the repeatability of the test method meets the requirements.

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Abstract

The invention discloses a method for determining the content of kaempferol-3-O-rutinoside in safflower, and belongs to the technical field of traditional Chinese medicine active ingredient content detection. Active ingredient, namely kaempferol-3-O-rutinoside in safflower is extracted by an organic solvent to serve as test solutions, the test solutions and a reference substance solution are determined by a high performance liquid chromatograph respectively, so that qualitative and quantitative detection of the active ingredient, namely kaempferol-3-O-rutinoside in safflower is realized, and the detection limit can reach 0.16 ug/mL; the method has the characteristics of simplicity and convenience in operation, high stability, good repeatability, strong specificity and the like, and providesa reliable detection method for subsequent qualitative and quantitative research of kaempferol-3-O-rutinoside in safflower, product quality control, evaluation index establishment and the like.

Description

technical field [0001] The invention relates to a method for determining the content of kaempferol-3-O-rutinoside in safflower, in particular to a method for determining the content of kaempferol-3-O-rutinoside in safflower by high performance liquid chromatography, The invention belongs to the technical field of content detection of active ingredients of traditional Chinese medicine. Background technique [0002] Safflower, Asteraceae, has the effects of promoting blood circulation, promoting menstrual flow, dispelling blood stasis and relieving pain, etc. It is generally used for amenorrhea, dysmenorrhea, lochia, lumps in the abdomen, chest pain and heartache, stagnant abdominal pain, hypochondriac pain, tumbling injuries and sores Sores, swelling, etc. The paper "Fei Yali, Huang Xiaomin, Research on the protective mechanism of safflower injection on endotoxin-induced acute liver injury in rats" mentioned that safflower injection can reduce the serum NO concentration in l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 赖小红李旺刘双李启发王江南夏柯钟钰刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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