Antineoplastic hydrogel polypeptide and preparation method

Abstract

The invention provides antineoplastic hydrogel polypeptide and a preparation method. The polypeptide comprises a self-assembling structural domain at the C end and a killing structural domain at the Nend, and the self-assembling structural domain is connected with the killing structural domain through a flexible structural domain. According to an antineoplastic melittin hydrogel slow-releasing agentia, the antineoplastic hydrogel polypeptide carries antineoplastic active polypeptide to obtain the agentia. The preparation method comprises the following steps that 1), an antineoplastic hydrogelpolypeptide aqueous solution and an antineoplastic active polypeptide aqueous solution are prepared; 2), the antineoplastic hydrogel polypeptide aqueous solution and the antineoplastic active polypeptide aqueous solution which are prepared in the step 1) are mixed to obtain a mixed solution; 3), an NaCl solution is dropwise added into the mixed solution prepared in the step S2 until the concentration of the NaCl solution is 0.9%, and after standing, the antineoplastic melittin hydrogel slow-releasing agentia is prepared. The technical purposes of promotion of the uptaking efficiency of activepolypeptide cells, cytoplasm location, preparation of the antineoplastic hydrogel polypeptide and the preparation method can be achieved.

Description

technical field [0001] The present invention relates to the field of biomedicine, and more particularly relates to an anti-tumor hydrogel polypeptide and a preparation method. The polypeptide can promote cell uptake efficiency and cytoplasmic localization of active polypeptides, and can self-assemble to form a hydrogel. Background technique [0002] At present, malignant tumors have seriously threatened human health. Traditional treatment methods (including surgery, chemotherapy, radiotherapy, etc.) not only have low selectivity, but also have high toxicity and side effects, and are prone to treatment resistance or resistance. Anti-tumor active polypeptides are a class of functional amino acid sequences. Various small molecular polypeptides can inhibit the growth of tumor cells through different mechanisms, and they have good biological safety and specificity. However, there are two main problems in anti-tumor applications: ① many active peptides cannot enter tumor cells eff...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide an anti-tumor hydrogel polypeptide and its preparation method, which can solve the problem of promoting the cell

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