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Detection method for discriminating baicalin and chlorogenic acid thin layer in compound honeysuckle flower particles

A compound honeysuckle and thin-layer identification technology, which is applied in the field of medical technology analysis, can solve problems that affect product production and quality assurance, cannot effectively control product quality, and unclear spots, etc., to shorten sample preparation time and accurately control sample volume , easy-to-operate effects

Inactive Publication Date: 2019-05-03
XIAN DAQING PHARMA FACTORY JINHUA ENTERPRISE GROUP CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is currently a commonly used medicine for clinical treatment of wind-heat and cold, and its curative effect is unquestionable. However, for the quality control of this product, the current national standard thin-layer identification method for baicalin and chlorogenic acid has poor specificity, and the spots are not clear, so it cannot effectively control product quality. , thereby affecting the production and quality assurance of products

Method used

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  • Detection method for discriminating baicalin and chlorogenic acid thin layer in compound honeysuckle flower particles
  • Detection method for discriminating baicalin and chlorogenic acid thin layer in compound honeysuckle flower particles
  • Detection method for discriminating baicalin and chlorogenic acid thin layer in compound honeysuckle flower particles

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Effect test

Embodiment 1

[0078] The compound honeysuckle granule baicalin and chlorogenic acid TLC identification detection method disclosed by the present invention comprises the following steps:

[0079] 1. Preparation of the test solution:

[0080] Take the sample and grind it finely, weigh 2.5g, add methanol 50mL, sonicate for 10 minutes, filter, take the filtrate and evaporate to dryness, add methanol 2mL to dissolve the residue, and complete the preparation of the test solution.

[0081] 2. Negative control solution preparation

[0082] Step a: According to the 5000g prescription, weigh 750g of forsythia (prescription without honeysuckle and scutellaria baicalensis), add 12 times the amount of water to extract twice, each time for 1 hour, combine the extracts, concentrate to a relative density of 1.26 (60~ 70°C), weigh, add 3 times the amount of ethanol, stir while adding, stand still for 24 hours, recover ethanol, concentrate to 1.26 (60-70°C) thick paste, add sucrose powder, mix well, and mak...

Embodiment 2

[0102] The compound honeysuckle granule baicalin and chlorogenic acid TLC identification detection method disclosed by the present invention comprises the following steps:

[0103] 1. Preparation of the test solution:

[0104] Take the sample and grind it finely, weigh 2.5g, add methanol 50mL, ultrasonically treat for 30 minutes, filter, take the filtrate and evaporate to dryness, add methanol 2mL to dissolve the residue, and complete the preparation of the test solution.

[0105] 2. Negative control solution preparation

[0106] Step a: According to the 5000g prescription, weigh 750g of forsythia (prescription without honeysuckle and scutellaria baicalensis), add 12 times the amount of water to extract twice, each time for 1 hour, combine the extracts, concentrate to a relative density of 1.26 (60~ 70°C), weigh, add 3 times the amount of ethanol, stir while adding, stand still for 24 hours, recover ethanol, concentrate to 1.26 (60-70°C) thick paste, add sucrose powder, mix w...

Embodiment 3

[0126] The compound honeysuckle granule baicalin and chlorogenic acid TLC identification detection method disclosed by the present invention comprises the following steps:

[0127] 1. Preparation of the test solution:

[0128] Take the sample and grind it finely, weigh 2.5g, add methanol 50mL, ultrasonically treat for 20 minutes, filter, take the filtrate and evaporate to dryness, add methanol 2mL to dissolve the residue, and complete the preparation of the test solution.

[0129] 2. Negative control solution preparation

[0130] Step a: According to the 5000g prescription, weigh 750g of forsythia (prescription without honeysuckle and scutellaria baicalensis), add 12 times the amount of water to extract twice, each time for 1 hour, combine the extracts, concentrate to a relative density of 1.26 (60~ 70°C), weigh, add 3 times the amount of ethanol, stir while adding, stand still for 24 hours, recover ethanol, concentrate to 1.26 (60-70°C) thick paste, add sucrose powder, mix w...

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Abstract

The invention provides a detection method for discriminating a baicalin and chlorogenic acid thin layer in compound honeysuckle flower particles. The method comprises the following steps that 1) a tested object solution, a negative control solution, a baicalin contrast solution and a chlorogenic acid contrast solution are prepared, a developing solvent and a developer are prepared, and the developing solvent comprises ethyl acetate, methanol and acetic acid in the volume ratio of (1-7): (2-5): (7-15); 2) thin layer chromatography is carried out by taking a polyamide thin film as a thin layer plate, dropping the tested object solution, negative control solution, baicalin contrast solution and chlorogenic acid contrast solution on the thin layer plate, placing the thin layer plate dropped with samples in a developing cylinder saturated with the developing agent to implement development, removing the thin layer plate, and air drying; and 3) chlorogenic acid and baicalin are inspected in UV and daylight respectively. The method can be discriminate thin layers of the baicalin and chlorogenic acid in the compound honeysuckle flower particles specifically.

Description

technical field [0001] The invention belongs to the field of medical technology analysis, in particular to a thin-layer identification and detection method for baicalin and chlorogenic acid in compound honeysuckle granules. Background technique [0002] Compound Honeysuckle Granules is a traditional Chinese medicine preparation refined by modern extraction of active ingredients from honeysuckle, forsythia and scutellaria baicalensis, and adding suitable sucrose. Its functions and indications are heat-clearing, detoxifying, cooling blood and reducing swelling. It is mainly used for anemopyretic cold, pharyngitis, tonsillitis, eye pain, toothache and carbuncle sores. It is currently a commonly used medicine for clinical treatment of wind-heat and cold, and its curative effect is unquestionable. However, for the quality control of this product, the current national standard thin-layer identification method for baicalin and chlorogenic acid has poor specificity, and the spots ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 薛美玲刘福敏陈美红王川吏郭维真
Owner XIAN DAQING PHARMA FACTORY JINHUA ENTERPRISE GROUP CORP
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